133 research outputs found
Towards nanowire sensors on a microfluidic platform: In-situ formation, positioning and sizing of nanowire bundles
Funding from the European Research Council under the 7th Framework Programme (ERC Strating Grant no. 203428 n”LIPIDS) is gratefully acknowledgedPeer Reviewe
Ultrahigh-Throughput Screening of an Artificial Metalloenzyme using Double Emulsions
The potential for ultrahigh-throughput compartmentalization renders droplet microfluidics an attractive tool for the directed evolution of enzymes. Importantly, it ensures maintenance of the phenotype-genotype linkage throughout optimization, enabling reliable identification of improved mutants. The full potential of droplet microfluidics remains unexplored, however, as droplet sorting often relies on low-throughput, custom-made devices that typically only allow simultaneous analysis of two parameters. Here, we report an approach for ultrahigh-throughput screening of an artificial metalloenzyme in double emulsion droplets (DEs) using commercially-available fluorescence-activated cell sorters (FACS). This protocol was validated by screening a 400 double-mutant streptavidin library for ruthenium-catalyzed deallylation of allocprotected aminocoumarin. The most active variants, identified by next generation sequencing, were in good agreement with hits obtained using a 96-well plate procedure. These findings pave the way for the systematic implementation of FACS for the directed evolution of enzymes and will significantly expand the accessibility of ultrahighthroughput DE screening protocols
UltrahighâThroughput Screening of an Artificial Metalloenzyme using Double Emulsions
The potential for ultrahigh-throughput compartmentalization renders droplet microfluidics an attractive tool for the directed evolution of enzymes. Importantly, it ensures maintenance of the phenotype-genotype linkage, enabling reliable identification of improved mutants. Herein, we report an approach for ultrahigh-throughput screening of an artificial metalloenzyme in double emulsion droplets (DEs) using commercially available fluorescence-activated cell sorters (FACS). This protocol was validated by screening a 400 double-mutant streptavidin library for ruthenium-catalyzed deallylation of an alloc-protected aminocoumarin. The most active variants, identified by next-generation sequencing, were in good agreement with hits obtained using a 96-well plate procedure. These findings pave the way for the systematic implementation of FACS for the directed evolution of (artificial) enzymes and will significantly expand the accessibility of ultrahigh-throughput DE screening protocols
MyCTC chip: microfluidic-based drug screen with patient-derived tumour cells from liquid biopsies
Cancer patients with advanced disease are characterized by intrinsic challenges in predicting drug response patterns, often leading to ineffective treatment. Current clinical practice for treatment decision-making is commonly based on primary or secondary tumour biopsies, yet when disease progression accelerates, tissue biopsies are not performed on a regular basis. It is in this context that liquid biopsies may offer a unique window to uncover key vulnerabilities, providing valuable information about previously underappreciated treatment opportunities. Here, we present MyCTC chip, a novel microfluidic device enabling the isolation, culture and drug susceptibility testing of cancer cells derived from liquid biopsies. Cancer cell capture is achieved through a label-free, antigen-agnostic enrichment method, and it is followed by cultivation in dedicated conditions, allowing on-chip expansion of captured cells. Upon growth, cancer cells are then transferred to drug screen chambers located within the same device, where multiple compounds can be tested simultaneously. We demonstrate MyCTC chip performance by means of spike-in experiments with patient-derived breast circulating tumour cells, enabling >95% capture rates, as well as prospective processing of blood from breast cancer patients and ascites fluid from patients with ovarian, tubal and endometrial cancer, where sensitivity to specific chemotherapeutic agents was identified. Together, we provide evidence that MyCTC chip may be used to identify personalized drug response patterns in patients with advanced metastatic disease and with limited treatment opportunities
Kazuma Mawatari, Takehiko Tsukahara, Yo Tanaka, Yutaka Kazoe, Philip Dextras and Takehiko Kitamori: Extended-nanofluidic systems for chemistry and biotechnology
ISSN:1618-2650ISSN:1618-264
Droplet microfluidics with magnetic beads: A new tool to investigate drugâprotein interactions
ISSN:1618-2650ISSN:1618-264
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