Additional file 5: Figure S2. of Hypomethylation of FAM63B in bipolar disorder patients

Scatterplot of PC1 and PC2 obtained from PCA of genome-wide independent SNPs (n = 39918, R 2 < 0.1) in UCL sample. (EPS 801 kb

Polymorphism in Serotonin Receptor 3B Is Associated with Pain Catastrophizing

<div><p>Pain catastrophizing, a coping style characterized by excessively negative thoughts and emotions in relation to pain, is one of the psychological factors that most markedly predicts variability in the perception of pain; however, only little is known about the underlying neurobiology. The aim of this study was to test for associations between psychological variables, such as pain catastrophizing, anxiety and depression, and selected polymorphisms in genes related to monoaminergic neurotransmission, in particular serotonin pathway genes. Three hundred seventy-nine healthy participants completed a set of psychological questionnaires: the Pain Catastrophizing Scale (PCS), the State-Trait Anxiety Inventory and Beck’s Depression Inventory, and were genotyped for 15 single nucleotide polymorphisms (SNPs) in nine genes. The SNP rs1176744 located in the serotonin receptor 3_B gene (<i>5-HTR3B</i>) was found to be associated with pain catastrophizing scores: both the global score and the subscales of magnification and helplessness. This is the first study to show an association between <i>5-HTR3B</i> and PCS scores, thus suggesting a role of the serotonin pathway in pain catastrophizing. Since <i>5-HTR3B</i> has previously been associated with descending pain modulation pathways, future studies will be of great interest to elucidate the molecular pathways involved in the relation between serotonin, its receptors and pain catastrophizing.</p></div

Additional file 3: Figure S1. of Hypomethylation of FAM63B in bipolar disorder patients

Technical assessment of the iPLEX method. Samples analyzed with iPLEX and pyrosequencing for FAM63B_2 site (panel A). Technical replicates for the FAM63B_2 assay run with iPLEX (panel B). (PDF 150 kb

Additional file 4: Table S3. of Hypomethylation of FAM63B in bipolar disorder patients

Overview for genotype counts for SNPs genotyped in FAM63B. (DOCX 39 kb

Additional file 2: Table S2. of Hypomethylation of FAM63B in bipolar disorder patients

Primer sequences for the two FAM63B iPLEX assays. (DOCX 43 kb

Additional file 9: Figure S5. of Hypomethylation of FAM63B in bipolar disorder patients

Expression of FAM63B across tissues according to BioGPS portal (http://biogps.org/). (PNG 137 kb

Additional file 7: Figure S3. of Hypomethylation of FAM63B in bipolar disorder patients

DNA methylation levels for cg21149266, an Illumina 450 K methylation array probe in the region of interest in FAM63B across different blood cell types [17]. (PNG 242 kb

Genetic polymorphisms genotyped in this study.

<p>Genetic polymorphisms genotyped in this study.</p

Minor allele association with personality trait scores.

<p>Regression coefficient (slopes of the regression) between the minor allele and the psychological trait. MA, minor allele; MAF, observed minor allele frequencies. In bold, significant associations after regression analysis using additive model and preserved after Bonferroni correction for multiple comparisons. PCS, pain catastrophizing scale; PCS R, pain catastrophizing rumination; PCS M, pain catastrophizing magnification; PCS H, pain catastrophizing helplessness. BDI, Beck’s Depression Inventory; STAI, State-Trait Anxiety Inventory I (state) and II (trait).</p

59_EuropeanWolves

Single nucleotide polymorphism (SNP) raw data files for 59 individuals in PLINK-format