A multiwavelength numerical model in support of quantitative retrievals of aerosol properties from automated lidar ceilometers and test applications for AOT and PM10 estimation

Abstract. The use of automated lidar ceilometer (ALC) systems for the aerosol vertically resolved characterization has increased in recent years thanks to their low construction and operation costs and their capability of providing continuous unattended measurements. At the same time there is a need to convert the ALC signals into usable geophysical quantities. In fact, the quantitative assessment of the aerosol properties from ALC measurements and the relevant assimilation in meteorological forecast models is amongst the main objectives of the EU COST Action TOPROF ("Towards operational ground-based profiling with ALCs, Doppler lidars and microwave radiometers for improving weather forecasts"). Concurrently, the E-PROFILE program of the European Meteorological Services Network (EUMETNET) focuses on the harmonization of ALC measurements and data provision across Europe. Within these frameworks, we implemented a model-assisted methodology to retrieve key aerosol properties (extinction coefficient, surface area, and volume) from elastic lidar and/or ALC measurements. The method is based on results from a large set of aerosol scattering simulations (Mie theory) performed at UV, visible, and near-IR wavelengths using a Monte Carlo approach to select the input aerosol microphysical properties. An average "continental aerosol type" (i.e., clean to moderately polluted continental aerosol conditions) is addressed in this study. Based on the simulation results, we derive mean functional relationships linking the aerosol backscatter coefficients to the abovementioned variables. Applied in the data inversion of single-wavelength lidars and/or ALCs, these relationships allow quantitative determination of the vertically resolved aerosol backscatter, extinction, volume, and surface area and, in turn, of the extinction-to-backscatter ratios (i.e., the lidar ratios, LRs) and extinction-to-volume conversion factor (cv) at 355, 532, and 1064 nm. These variables provide valuable information for visibility, radiative transfer, and air quality applications. This study also includes (1) validation of the model simulations with real measurements and (2) test applications of the proposed model-based ALC inversion methodology. In particular, our model simulations were compared to backscatter and extinction coefficients independently retrieved by Raman lidar systems operating at different continental sites within the European Aerosol Research Lidar Network (EARLINET). This comparison shows good model–measurement agreement, with LR discrepancies below 20 %. The model-assisted quantitative retrieval of both aerosol extinction and volume was then tested using raw data from three different ALCs systems (CHM 15k Nimbus), operating within the Italian Automated LIdar-CEilometer network (ALICEnet). For this purpose, a 1-year record of the ALC-derived aerosol optical thickness (AOT) at each site was compared to direct AOT measurements performed by colocated sun–sky photometers. This comparison shows an overall AOT agreement within 30 % at all sites. At one site, the model-assisted ALC estimation of the aerosol volume and mass (i.e., PM10) in the lowermost levels was compared to values measured at the surface level by colocated in situ instrumentation. Within this exercise, the ALC-derived daily-mean mass concentration was found to reproduce the corresponding (EU regulated) PM10 values measured by the local air quality agency well in terms of both temporal variability and absolute values. Although limited in space and time, the good performances of the proposed approach suggest it could possibly represent a valid option to extend the capabilities of ALCs to provide quantitative information for operational air quality and meteorological monitoring

Atmospheric Thermodynamic Profiling through the Use of a Micro-Pulse Raman Lidar System: Introducing the Compact Raman Lidar MARCO

It was for a long time believed that lidar systems based on the use of high-repetition micro-pulse lasers could be effectively used to only stimulate atmospheric elastic backscatter echoes, and thus were only exploited in elastic backscatter lidar systems. Their application to stimulate rotational and roto-vibrational Raman echoes, and consequently, their exploitation in atmospheric thermodynamic profiling, was considered not feasible based on the technical specifications possessed by these laser sources until a few years ago. However, recent technological advances in the design and development of micro-pulse lasers, presently achieving high UV average powers (1–5 W) and small divergences (0.3–0.5 mrad), in combination with the use of large aperture telescopes (0.3–0.4 m diameter primary mirrors), allow one to presently develop micro-pulse laser-based Raman lidars capable of measuring the vertical profiles of atmospheric thermodynamic parameters, namely water vapor and temperature, both in the daytime and night-time. This paper is aimed at demonstrating the feasibility of these measurements and at illustrating and discussing the high achievable performance level, with a specific focus on water vapor profile measurements. The technical solutions identified in the design of the lidar system and their technological implementation within the experimental setup of the lidar prototype are also carefully illustrated and discussed

Novel aromatic ring-hydroxylating dioxygenase genes from coastal marine sediments of Patagonia

<p>Abstract</p> <p>Background</p> <p>Polycyclic aromatic hydrocarbons (PAHs), widespread pollutants in the marine environment, can produce adverse effects in marine organisms and can be transferred to humans through seafood. Our knowledge of PAH-degrading bacterial populations in the marine environment is still very limited, and mainly originates from studies of cultured bacteria. In this work, genes coding catabolic enzymes from PAH-biodegradation pathways were characterized in coastal sediments of Patagonia with different levels of PAH contamination.</p> <p>Results</p> <p>Genes encoding for the catalytic alpha subunit of aromatic ring-hydroxylating dioxygenases (ARHDs) were amplified from intertidal sediment samples using two different primer sets. Products were cloned and screened by restriction fragment length polymorphism analysis. Clones representing each restriction pattern were selected in each library for sequencing. A total of 500 clones were screened in 9 gene libraries, and 193 clones were sequenced. Libraries contained one to five different ARHD gene types, and this number was correlated with the number of PAHs found in the samples above the quantification limit (<it>r </it>= 0.834, <it>p </it>< 0.05). Overall, eight different ARHD gene types were detected in the sediments. In five of them, their deduced amino acid sequences formed deeply rooted branches with previously described ARHD peptide sequences, exhibiting less than 70% identity to them. They contain consensus sequences of the Rieske type [2Fe-2S] cluster binding site, suggesting that these gene fragments encode for ARHDs. On the other hand, three gene types were closely related to previously described ARHDs: archetypical <it>nahAc</it>-like genes, <it>phnAc</it>-like genes as identified in <it>Alcaligenes faecalis </it>AFK2, and <it>phnA1</it>-like genes from marine PAH-degraders from the genus <it>Cycloclasticus</it>.</p> <p>Conclusion</p> <p>These results show the presence of hitherto unidentified ARHD genes in this sub-Antarctic marine environment exposed to anthropogenic contamination. This information can be used to study the geographical distribution and ecological significance of bacterial populations carrying these genes, and to design molecular assays to monitor the progress and effectiveness of remediation technologies.</p

The hyperornithinemia-hyperammonemia-homocitrullinuria syndrome

Hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome is a rare autosomal recessive disorder of the urea cycle. HHH has a panethnic distribution, with a major prevalence in Canada, Italy and Japan. Acute clinical signs include intermittent episodes of vomiting, confusion or coma and hepatitis-like attacks. Alternatively, patients show a chronic course with aversion for protein rich foods, developmental delay/intellectual disability, myoclonic seizures, ataxia and pyramidal dysfunction. HHH syndrome is caused by impaired ornithine transport across the inner mitochondrial membrane due to mutations in SLC25A15 gene, which encodes for the mitochondrial ornithine carrier ORC1. The diagnosis relies on clinical signs and the peculiar metabolic triad of hyperammonemia, hyperornithinemia, and urinary excretion of homocitrulline. HHH syndrome enters in the differential diagnosis with other inherited or acquired conditions presenting with hyperammonemia

CUGC for hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome

From 1999 to date, 50 affecting function variants have been identified and associated to HHH syndrome [1–5]. As it is not available in the literature a complete up-to-date list of disease-causing variants for SLC25A15 gene, we included this information as a Supplementary Excel sheet (See Supplementary Material File #1): this list was created by using LOVD and ClinVar databases and liked to the relevant literature reference. Reported variants consist of: 29 missense variants, 4 frameshift, 11 nonsense, 2 splicing, 2 small deletion, 1 in frame insertion, 1 gross deletion

The ketogenic diet increases in vivo glutathione levels in patients with epilepsy

The Ketogenic Diet (KD) is a high-fat, low-carbohydrate diet that has been utilized as the first line treatment for contrasting intractable epilepsy. It is responsible for the presence of ketone bodies in blood, whose neuroprotective effect has been widely shown in recent years but remains unclear. Since glutathione (GSH) is implicated in oxidation-reduction reactions, our aim was to monitor the effects of KD on GSH brain levels by means of magnetic resonance spectroscopy (MRS). MRS was acquired from 16 KD patients and seven age-matched Healthy Controls (HC). We estimated metabolite concentrations with linear combination model (LCModel), assessing differences between KD and HC with t-test. Pearson was used to investigate GHS correlations with blood serum 3-B-Hydroxybutyrate (3HB) concentrations and with number of weekly epileptic seizures. The results have shown higher levels of brain GSH for KD patients (2.5 ± 0.5 mM) compared to HC (2.0 ± 0.5 mM). Both blood serum 3HB and number of seizures did not correlate with GSH concentration. The present study showed a significant increase in GSH in the brain of epileptic children treated with KD, reproducing for the first time in humans what was previously observed in animal studies. Our results may suggest a pivotal role of GSH in the antioxidant neuroprotective effect of KD in the human brain

Impact of the different preparation methods to obtain autologous non\u2010activated platelet\u2010rich plasma (A\u2010PRP) and activated platelet\u2010rich plasma (AA\u2010PRP) in plastic surgery: Wound healing and hair regrowth evaluation

Autologous therapies using platelet\u2010rich plasma (PRP) need meticulous preparation\u2014currently, no standardised preparation technique exists. Processing Quantitative Standards (PQSs) define manufacturing quantitative variables (such as time, volume and pressure). Processing Qualitative Standards (PQLSs) define the quality of the materials and methods of manufacturing. The aim of this review is to use existing PQSs and PQLs to report the in vivo/in vitro results obtained by using different Kits, that utilise different procedures (classified as Closed\u2010Technique and Opened\u2010Technique) to isolate autologous human activated (AA\u2010PRP) or non\u2010activated PRP (A\u2010PRP). PQSs included the volumes of blood collected as well as the reagents used, the time/gravity of centrifugation, and the duration, temperature and tilt level/speed of centrifugation. PQLSs included the use of Calcium Chloride CaCl2, Kit weight, transparency of Kit components, the maintenance of a closed sterile processing environment and the use of a small centrifuge. Eight CE marked devices for PRP extraction were evaluated: Angel\uae, Biomed\uae, Cascade\uae and Selphyl\uae, Mag\u201018\uae, i\u2010Stem\uae, MyCells\uae and Regenlab\uae. Using a Kit with the PQSs and PQLSs described in this study enables the isolation of A\u2010PRP, thereby meeting consensus quality criteria. As our understanding of Critical Quality Attributes (CQAs) of A\u2010PRP continues to evolve, especially with respect to purity and potency, adjustments to these benchmark PQSs and PQLs will hopefully help isolate A\u2010PRP of desired CQAs with greater reproducibility, quality, and safety. Confirmatory studies will no doubt need to be completed