Enhanced physicochemical and biological properties of ion-implanted Titanium using Electron Cyclotron Resonance ion sources

The surface properties of metallic implants play an important role in their clinical success. Improving upon the inherent shortcomings of Ti implants, such as poor bioactivity, is imperative for achieving clinical use. In this study, we have developed a Ti implant modified with Ca or dual Ca + Si ions on the surface using an electron cyclotron resonance ion source (ECRIS). The physicochemical and biological properties of ion-implanted Ti surfaces were analyzed using various analytical techniques, such as surface analyses, potentiodynamic polarization and cell culture. Experimental results indicated that a rough morphology was observed on the Ti substrate surface modified by ECRIS plasma ions. The in vitro electrochemical measurement results also indicated that the Ca + Si ion-implanted surface had a more beneficial and desired behavior than the pristine Ti substrate. Compared to the pristine Ti substrate, all ion-implanted samples had a lower hemolysis ratio. MG63 cells cultured on the high Ca and dual Ca + Si ion-implanted surfaces revealed significantly greater cell viability in comparison to the pristine Ti substrate. In conclusion, surface modification by electron cyclotron resonance Ca and Si ion sources could be an effective method for Ti implants

Synergistic reinforcement of surface modification on improving the bonding of veneering ceramics to zirconia

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Rheology, crystallization behavior, and mechanical properties of poly (butylene succinate-co-terephthalate)/cellulose nanocrystal composites

A series of biodegradable poly (butylene succinate-co-terephthalate) (PBST) with different aromatic units content was synthesized and then melt blended by adding cellulose nanocrystals (CNCs) to manufacture the full organic composites. A network-like structure of CNCs in PBST matrix was evaluated by rheometer. The storage modulus and complex viscosity at low frequency region were significantly enhanced with increasing CNC content. Meanwhile, the decreasing and flow index were attributed to the excellent interaction between PBST and CNCs. When PBST has a content of the aromatic unit exceeds 30 mol%, the crystallization temperatures increased with increasing CNC contents. On the other hand, when PBST has 30 mol% content of the aromatic unit, the cold crystallization temperatures decreased with increasing CNC contents. These above observation in crystallization properties suggested that the CNC make a role of heterogeneous nucleation in PBST matrix. The result of mechanical properties evaluated by dynamic mechanical analysis showed a good reinforcement effect of the addition of stiff CNC. The PBST/CNC composites were suitable for cell growth and might have a potential as biomedical materials, which is confirmed by MTT assay

Comparative Osteogenesis of Radiopaque Dicalcium Silicate Cement and White-Colored Mineral Trioxide Aggregate in a Rabbit Femur Model

The radiopaque dicalcium silicate cement (RDSC) displayed a shortened setting time and good biocompatibility. This study aimed to compare the regenerative potential of RDSC and white-colored mineral trioxide aggregate (WMTA) using a rabbit femur model. The animals were sacrificed at one, three and six months to accomplish histological and biochemical analyses. The results indicated that after one month of implantation, WMTA was associated with a greyish color alteration within its mass, while RDSC presented color stability even at six months. Histological assay with Masson’s Trichrome and Von Kossa stains showed the presence of newly formed bone surrounding the implanted sites in the rabbit femur. The histochemical data revealed that the RDSC group had significantly more bone regeneration than did the WMTA groups at three and six months. The conclusion drawn is that the encouraging results support the potential applications of RDSC as an improved alternative to WMTA for endodontic uses

Effectiveness of Hypochlorous Acid to Reduce the Biofilms on Titanium Alloy Surfaces in Vitro

Chemotherapeutic agents have been used as an adjunct to mechanical debridement for peri-implantitis treatment. The present in vitro study evaluated and compared the effectiveness of hypochlorous acid (HOCl), sodium hypochlorite (NaOCl), and chlorhexidine (CHX) at eliminating Gram-negative (E. coli and P. gingivalis) and Gram-positive (E. faecalis and S. sanguinis) bacteria. The effect of irrigating volume and exposure time on the antimicrobial efficacy of HOCl was evaluated, and a durability analysis was completed. Live/dead staining, morphology observation, alamarBlue assay, and lipopolysaccharide (LPS) detection were examined on grit-blasted and biofilm-contaminated titanium alloy discs after treatment with the three chemotherapeutic agents. The results indicated that HOCl exhibited better antibacterial efficacy with increasing irrigating volumes. HOCl achieved greater antibacterial efficacy as treatment time was increased. A decrease in antimicrobial effectiveness was observed when HOCl was unsealed and left in contact with the air. All the irrigants showed antibacterial activity and killed the majority of bacteria on the titanium alloy surfaces of biofilm-contaminated implants. Moreover, HOCl significantly lowered the LPS concentration of P. gingivalis when compared with NaOCl and CHX. Thus, a HOCl antiseptic may be effective for cleaning biofilm-contaminated implant surfaces

The Effect of Abutment Angulation and Crown Material Compositions on Stress Distribution in 3-Unit Fixed Implant-Supported Prostheses: A Finite Element Analysis

Objective. The aim of this study was to evaluate influence of abutment angulation and restoration material compositions on the stress pattern in dental implants and their surrounding bone. Materials and Methods. In this finite element study, the six different solid 3D models of "mandibular 3-unit fixed implant-supported prostheses" were analyzed. In all of these models, a straight abutment was used for anterior implants at the second premolar site, and in order to posterior implant at the second molar site, abutments with three different angles (straight, 15, and 20 degrees) were used. Also, two different restoration material compositions (porcelain fused to base metal (PFBM) and porcelain fused to noble metal (PFNM)) were considered for fixed implant supported restorations. A 450 N static force was exerted in a straight manner along the longitudinal axis of the anterior implant in a tripod, and the stress distribution was measured based on the restoration materials and abutment angulations of the models in the 3 sites of cortical, cancellous bone, and fixtures. The simulation was performed with ABAQUS 6.13 Software. Results. In all models, stress values in surrounding cortical bone were more than in spongy bone. Maximum stress levels in an anterior abutment-implant complex were seen in models with angled implants. In models with parallel implants, the stress level of a molar straight abutment-implant complex was less than that of premolar straight ones. In an angled posterior abutment-implant complex, less stress level was detected compared to straight ones. In all PFNB models, stress values were slightly more and distributed in a wider area of premolar straight abutments. Conclusion. Increasing an abutment angle, increases stress in surrounding bone and straight implant-abutment combination. It seems that the crown material composition affects stress distribution of the implant-abutment combination but does not affect stress distribution of surrounding bone

Effect of polydimethylsiloxane surfaces silanized with different nitrogen-containing groups on the adhesion progress of epithelial cells

There have been few studies on the effect of the grafting of nitrogen-containing functional groups on polydimethylsiloxane (PDMS) surfaces and, in particular, the CN bond, on cell adhesion to the surfaces. In this study, two different silanes, one containing an amine group [(3-aminopropyl)trimethoxysilane; APTMS] and the other a nitrile group [(3-cyanopropyl)triethoxysilane; CPTES], were adopted to modify PDMS following oxygen plasma treatment. The effects of surface modification on the density of HeLa and MDCK cells were evaluated, in addition to the measurement of the contact angle with the surface and the chemical composition. The results indicated that the CPTES modification allowed the PDMS surface to have better hydrophilicity than the APTMS modification. Nitrogen-containing functionalities were more beneficial for cell spreading, in comparison with the pristine and oxygen plasma-treated PDMS. The present method of APTMS and CPTES grafting provides a simple and efficient method for promoting cell adhesion on PDMS. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved

Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium

Intensive efforts have been made to eliminate or substantial reduce bacterial adhesion and biofilm formation on titanium implants. However, in the management of peri-implantitis, the methylene blue (MB) photosensitizer commonly used in photoantimicrobial chemotherapy (PACT) is limited to a low retention on the implant surface. The purpose of this study was to assess enhancive effect of water-soluble quaternary ammonium chitosan (QTS) on MB retention on biofilm-infected SLA (sandblasted, large grid, and acid-etched) Ti alloy surfaces in vitro. The effectiveness of QTS + MB with different concentrations in eliminating Gram-negative A. actinomycetemcomitans or Gram-positive S. mutans bacteria was compared before and after PACT. Bacterial counting and lipopolysaccharide (LPS) detection were examined, and then the growth of human osteoblast-like MG63 cells was evaluated. The results indicated that the synergistic QTS + MB with retention ability significantly decreased the biofilm accumulation on the Ti alloy surface, which was better than the same concentration of 1 wt% methyl cellulose (MC). More importantly, the osteogenic activity of MG63 cells on the disinfected sample treated by QTS + MB-PACT modality was comparable to that of sterile Ti control, significantly higher than that by MC + MB-PACT modality. It is concluded that, in terms of improved retention efficacy, effective bacteria eradication, and enhanced cell growth, synergistically, PACT using the 100 μg/mL MB-encapsulated 1% QTS was a promising modality for the treatment of peri-implantitis