Measuring the Social-Ecological Resilience of Coastal and Small Island Communities to Inform Policy, Planning, and Practice

This study was developed in order to arrive at a set of interrelated concepts and empirical ways of measuring social-ecological resilience that are concretely applicable for policy, as well as for developing intervening programs for social change. The outcome of this research is a set of empirical indicators to measure the concept of social-ecological resilience. The measurement model is developed and applied to U.S. Caribbean and Pacific small island communities and U.S. Gulf of Mexico coastal counties (n=229), but is intended to be applicable across different types of communities with minor adjustments for the specific context. The first phase of this research resulted in a conceptual framework for the social ecological system and the property of resilience. Next, multiple methodological approaches to indicator construction were applied and directly compared. An iterative methodology was selected and applied to arrive at seven composite indicators of social-ecological resilience: Land cover and use, Waste accumulation and treatment, Housing adequacy, Economic security, Access to support services, Education, and Population diversity. Upon construction, the indicators were applied with two distinct samples of communities. Finally, the indicators were used to construct a community typology to account for the different strengths and weaknesses of small island and coastal communities as assessed by the indicators of social-ecological resilience. Communities with high scores on social dimensions of resilience have a greater likelihood of having low scores on ecological dimensions. This finding adds evidence to the notion that social and ecological systems are oppositional, but also provides a counterpoint – there are communities that manage to score well in both areas. While societal development and ecological condition may operate with a firm tension, communities are navigating the tension and finding ways to successfully maintain characteristics of resilience. This research is a necessary first step to investigating how some communities are able to balance their social-ecological system while others are not. Ultimately, the measurement of resilience can provide communities of island and coastal states with a way of evaluating their ability to implement, adapt, and/or support policies for change

Monitoring well-being and changing environmental conditions in coastal communities: development of an assessment method

The intersection of social and environmental forces is complex in coastal communities. The well-being of a coastal community is caught up in the health of its environment, the stability of its economy, the provision of services to its residents, and a multitude of other factors. With this in mind, the project investigators sought to develop an approach that would enable researchers to measure these social and environmental interactions. The concept of well-being proved extremely useful for this purpose. Using the Gulf of Mexico as a regional case study, the research team developed a set of composite indicators to be used for monitoring well-being at the county-level. The indicators selected for the study were: Social Connectedness, Economic Security, Basic Needs, Health, Access to Social Services, Education, Safety, Governance, and Environmental Condition. For each of the 37 sample counties included in the study region, investigators collected and consolidated existing, secondary data representing multiple aspects of objective well-being. To conduct a longitudinal assessment of changing wellbeing and environmental conditions, data were collected for the period of 2000 to 2010. The team focused on the Gulf of Mexico because the development of a baseline of well-being would allow NOAA and other agencies to better understand progress made toward recovery in communities affected by the Deepwater Horizon oil spill. However, the broader purpose of the project was to conceptualize and develop an approach that could be adapted to monitor how coastal communities are doing in relation to a variety of ecosystem disruptions and associated interventions across all coastal regions in the U.S. and its Territories. The method and models developed provide substantial insight into the structure and significance of relationships between community well-being and environmental conditions. Further, this project has laid the groundwork for future investigation, providing a clear path forward for integrated monitoring of our nation’s coasts. The research and monitoring capability described in this document will substantially help counties, local organizations, as well state and federal agencies that are striving to improve all facets of community well-being

Characterization of the Single Stranded DNA Binding Protein SsbB Encoded in the Gonoccocal Genetic Island

Background: Most strains of Neisseria gonorrhoeae carry a Gonococcal Genetic Island which encodes a type IV secretion system involved in the secretion of ssDNA. We characterize the GGI-encoded ssDNA binding protein, SsbB. Close homologs of SsbB are located within a conserved genetic cluster found in genetic islands of different proteobacteria. This cluster encodes DNA-processing enzymes such as the ParA and ParB partitioning proteins, the TopB topoisomerase, and four conserved hypothetical proteins. The SsbB homologs found in these clusters form a family separated from other ssDNA binding proteins. Methodology/Principal Findings: In contrast to most other SSBs, SsbB did not complement the Escherichia coli ssb deletion mutant. Purified SsbB forms a stable tetramer. Electrophoretic mobility shift assays and fluorescence titration assays, as well as atomic force microscopy demonstrate that SsbB binds ssDNA specifically with high affinity. SsbB binds single-stranded DNA with minimal binding frames for one or two SsbB tetramers of 15 and 70 nucleotides. The binding mode was independent of increasing Mg 2+ or NaCl concentrations. No role of SsbB in ssDNA secretion or DNA uptake could be identified, but SsbB strongly stimulated Topoisomerase I activity

Coastal natural and nature-based features: international guidelines for flood risk management

Natural and nature-based features (NNBF) have been used for more than 100 years as coastal protection infrastructure (e.g., beach nourishment projects). The application of NNBF has grown steadily in recent years with the goal of realizing both coastal engineering and environment and social co-benefits through projects that have the potential to adapt to the changing climate. Technical advancements in support of NNBF are increasingly the subject of peer-reviewed literature, and guidance has been published by numerous organizations to inform technical practice for specific types of nature-based solutions. The International Guidelines on Natural and Nature-Based Features for Flood Risk Management was recently published to provide a comprehensive guide that draws directly on the growing body of knowledge and practitioner experience from around the world to inform the process of conceptualizing, planning, designing, engineering, and operating NNBF. These Guidelines focus on the role of nature-based solutions and natural infrastructure (beaches, dunes, wetlands and plant systems, islands, reefs) as a part of coastal and riverine flood risk management. In addition to describing each of the NNBF types, their use, design, implementation, and maintenance, the guidelines describe general principles for employing NNBF, stakeholder engagement, monitoring, costs and benefits, and adaptive management. An overall systems approach is taken to planning and implementation of NNBF. The guidelines were developed to support decision-makers, project managers, and practitioners in conceptualizing, planning, designing, engineering, implementing, and maintaining sustainable systems for nature-based flood risk management. This paper summarizes key concepts and highlights challenges and areas of future research

SCUBA divers above the waterline: using participatory mapping of coral reef conditions to inform reef management

Coral reefs provide important ecological services such as biodiversity, climate regulation, and cultural benefits through recreation and tourism. However, many of the world's reefs are declining, with Caribbean reefs suffering a significant decline in living corals over the past half century. This situation emphasizes the need to assess and monitor reef conditions using a variety of methods. In this study, a new method for assessing reef conditions to inform management using participatory mapping by coral reef “experts” in the U.S. Virgin Islands (USVI) is described. Occupational SCUBA divers were recruited (n=87) to map coral reef conditions, uses, and threats (stressors) using an internet-based mapping website. The data reveal an uneven geographic distribution of reef conditions in the USVI with the most frequently mapped perceived healthy reef characteristics being: large amount of physical reef structure (n=872 markers); endangered or threatened species present (n=721); and large amount of live coral cover (n=615). The greatest perceived threats were: invasive species (n=606); water pollution (n=234); and unsustainable fishing (n=200). Areas of important reef characteristics, perceived threats to reefs, and perceived recovery potential were plotted to identify areas requiring critical management attention. The authors found that perceptions of healthy reef conditions outnumbered perceptions of reef threats for nine of the ten most familiar coral reefs; the most frequent activity type within the coral reefs was tourism diving; and for the most familiar coral reefs, the divers perceived a high recovery potential. Given the novelty of participatory mapping methods to assess coral reefs, the strengths and weaknesses of the method is evaluated. The authors further propose a management typology for categorizing reef areas to inform their future management. In the absence of primary data, or, as a supplement to underwater surveys and remotely-sensed data on reef condition, participatory mapping can provide a cost-effective means for assessing coral reef conditions while identifying place-specific reef locations requiring management attention

Autopsy Findings in 32 Patients with COVID-19: A Single-Institution Experience

A novel coronavirus, SARS-CoV-2, was identified in Wuhan, China in late 2019. This virus rapidly spread around the world causing disease ranging from minimal symptoms to severe pneumonia, which was termed coronavirus disease (i.e., COVID). Postmortem examination is a valuable tool for studying the pathobiology of this new infection. We report the clinicopathologic findings from 32 autopsy studies conducted on patients who died of COVID-19 including routine gross and microscopic examination with applicable special and immunohistochemical staining techniques. SARS-CoV-2 infection was confirmed by nasopharyngeal RT-PCR in 31 cases (97%) and by immunohistochemical staining for SARS-CoV-2 spike-protein in the lung in the remaining 1 case (3%). The ethnically diverse cohort consisted of 22 males and 10 females with a mean age of 68 years (range: 30-100). Patients most commonly presented with cough (17 [55%]), shortness of breath (26 [81%]), and a low-grade fever (17 [55%]). Thirty-one (97%) of the patients had at least 1 comorbidity (mean = 4). Twenty-eight patients (88%) had widespread thromboembolic disease, as well as diffuse alveolar damage (30 [94%]), diabetic nephropathy (17 [57%]) and acute tubular injury. Patterns of liver injury were heterogeneous, featuring 10 (36%) with frequent large basophilic structures in sinusoidal endothelium, and increased immunoblast-like cells in lymph nodes. This series of autopsies from patients with COVID-19 confirms the observation that the majority of severely affected patients have significant pulmonary pathology. However, many patients also have widespread microscopic thromboses, as well as characteristic findings in the liver and lymph nodes

Functional Analysis of the Gonococcal Genetic Island of <i>Neisseria gonorrhoeae</i>

<div><p><i>Neisseria gonorrhoeae</i> is an obligate human pathogen that is responsible for the sexually-transmitted disease gonorrhea. <i>N. gonorrhoeae</i> encodes a T4SS within the Gonococcal Genetic Island (GGI), which secretes ssDNA directly into the external milieu. Type IV secretion systems (T4SSs) play a role in horizontal gene transfer and delivery of effector molecules into target cells. We demonstrate that GGI-like T4SSs are present in other β-proteobacteria, as well as in α- and γ-proteobacteria. Sequence comparison of GGI-like T4SSs reveals that the GGI-like T4SSs form a highly conserved unit that can be found located both on chromosomes and on plasmids. To better understand the mechanism of DNA secretion by <i>N. gonorrhoeae</i>, we performed mutagenesis of all genes encoded within the GGI, and studied the effects of these mutations on DNA secretion. We show that genes required for DNA secretion are encoded within the <i>yaa-atlA</i> and <i>parA-parB</i> regions, while genes encoded in the <i>yfeB-exp1</i> region could be deleted without any effect on DNA secretion. Genes essential for DNA secretion are encoded within at least four different operons.</p></div

Schematic representation of the genetic map of the GGI of <i>N. gonorrhoeae</i>.

<p>Genes that have been previously characterized are colored. Red and orange indicate genes in which mutations resulted in a decrease in DNA secretion. Green indicates genes in which mutations had no effect on DNA secretion. For the genes colored in red, secretion could be restored by complementation. For the genes colored in orange no complementation was performed. The dot indicates the origin of transfer (<i>oriT</i>). difA and difB indicate the respective dif sites flanking the GGI.</p