Personality and social education and its influence on the classroom climate

This work is focused on the topic of school class climate and its enhancement by the methods of personality and social education in a concrete class where the author is working as a teacher. The theoretical part is dedicated to the class climate and its characteristics. It describes what influences, by which methods it can be investigated and what can it be influenced by. In the practical part the author uses the methods of the teacher's active research to investigate the climate in her own class and also self-reflexively examines herself from the view of the beginning teacher. During the time period of one school year continually monitors the climate in her class and states the most effective ways towards improvement. The results of the research show that the methods of personality and social education have an influence to the class climate if they are used in a natural and casual manner. KEY WORDS: Class climate, teacher's active research, beginning teacher, social skills, self - reflection, begin together, personality and social education, cooperation, relations, pre - puberty, problematic pupil, method

Intercompany mentoring

The diploma thesis is dedicated to the intercompany mentoring issue, its thorough description and specific practical application in the company. The objectives are to define the intercompany mentoring as a new form of corporate learning, to analyze it in detail and to assess its benefits to the certain company. Its strengths / weaknesses and the possible opportunities and threats are found in the theoretical part via SWOT analysis. The assessment of intercompany mentoring is based on the interviews with mentors, mentees and last but not least with mentees' superiors. These semi-structured interviews are focused on finding of the strengths / weaknesses and the opportunities or threats. It means that the SWOT analysis is used also in the practical part of the thesis. The output of the thesis is the final resume and my personal improvement proposals. The results of the thesis can help those companies that are looking for new ways to develop their employees or which are hesitating to participate in the intercompany mentoring. KEYWORDS Learning, development, intercompany, mentoring, mentor, mentee, HR, coordinator, company culture, code of ethic

MOESM4 of Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains

Additional file 4: Figure S4. Second derivatives of the FTIR absorption spectra of S. cerevisiae BY4741 and opi1Δ cells, in the absence of lactic acid: exponential phase. Cells were grown in shake flasks in minimal (YNB) medium with 2 % w/v glucose at pH3. FTIR analysis was performed at 18 h after the inoculation, corresponding to the exponential phase of growth. a: amide I band; b: vibrational modes mainly due to lipid CH2/CH3 and to phosphate groups, c: stretching modes from lipid hydrocarbon tails. In a and b second derivative spectra have been normalized to the tyrosine band at ~ 1516 cm−1, while in c spectra have been normalized at the CH3 band at ~ 2959 cm−1

MOESM5 of Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains

Additional file 5: Figure S5. Second derivatives of the FTIR absorption spectra of S. cerevisiae BY4741 and opi1Δ cells, in the presence of lactic acid: exponential phase. Cells were grown in shake flasks in minimal (YNB) medium with 2 % w/v glucose in the presence of 46 g/L of lactic acid (LA) at pH3. FTIR analysis was performed at 18 h after the inoculation, corresponding to the exponential phase of growth. a: amide I band; b: vibrational modes mainly due to lipid CH2/CH3 and to phosphate groups, c: stretching modes from lipid hydrocarbon tails. In a and b second derivative spectra have been normalized to the tyrosine band at ~ 1516 cm−1, while in c spectra have been normalized at the CH3 band at ~ 2959 cm−1

MOESM1 of Assessing an effective feeding strategy to optimize crude glycerol utilization as sustainable carbon source for lipid accumulation in oleaginous yeasts

Additional file 1. Additional Figures and Tables; Figures S1–S6, Tables S1–S2. Additional information; optimization of the feeding strategy

Dynamic light scattering and second derivative FTIR spectra of non-pegylated Met-G-CSF and of the two isomeric Met-G-CSF-Met1-PEG and Met-G-CSF-Gln135-PEG.

<p>A) Light scattering intensity, in arbitrary units, as a function of the incubation time at 55°C. At the zero time the bath temperature was changed from 37°C to 55°C. B) Polydispersity index (Eq.3) of the non-pegylated Met-G-CSF and of the two isomeric pegylated proteins as a function of the incubation time. C) Distribution of the hydrodynamic radii corresponding to the most abundant component in the DLS decay. The solid and dashed lines are best fit Gaussian function to the data that corresponds to Rh = 39.4±4 nm and Rh = 70±20 nm for the pegylated and non-pegylated samples respectively. D) Met-G-CSF, Met-G-CSF-Met1-PEG and Met-G-CSF-Gln135-PEG second derivative FTIR spectra measured after 7 hours of incubation at 55°C.Concerning the size of the protein aggregates, the diffusion coefficients (Eq.1) were used to evaluate the protein aggregate hydrodynamic radii through Eq.2. Non-pegylated Met-G-CSF displayed an average hydrodynamic radius of 1.4±0.4 nm at 37°C with negligible presence of protein aggregates. At 55°C, after thermal equilibrium was reached, we found instead a prevalent component with R_h = 70±20 nm. It should be noted that the value of 70 nm represents the average size of protein aggregates still in solution after 7 hours of incubation at 55^oC.</p

Thermal stability of Met-G-CSF and of the two pegylated forms by CD and fluorescence spectroscopies.

<p>A) Temperature dependence of the CD intensity at 222 nm. B) Temperature dependence of the HT[V] intensity at 222 nm. C) Temperature dependence of the fluorescence emission at 330 nm (excitation at 295 nm). D) Temperature dependence of the ratio between the fluorescence emission at 330 nm and at 350 nm (excitation at 295 nm).</p

FTIR spectroscopy analysis of Met-G-CSF and of the two pegylated forms.

<p>A) FTIR spectra of non-pegylated Met-G-CSF and of the two isomeric Met-G-CSF-Met1-PEG and Met-G-CSF-Gln135-PEG. B) Second derivative spectra in the Amide I band of the three proteins as in A).</p

Thermal stability of Met-G-CSF and of the two pegylated forms by CD spectroscopy.

<p>CD spectra of non-pegylated Met-G-CSF and of the two isomeric Met-G-CSF-Met1-PEG and Met-G-CSF-Gln135-PEG at 20°C (continuous line), 95°C (dashed line), and 20°C after heating up to 95°C (dotted line).</p