Effects of Electromagnetic Waves Emitted by Mobile Phones on Germination, Root Growth, and Root Tip Cell Mitotic Division of Lens culinaris Medik

In this study, the effects of electromagnetic waves emitted from mobile phones operating at 1800 MHz were investigated on germination, root growth and mitotic division of root tips of Lens culinaris Medik. Seeds were split into three groups. The first group was exposed to a mobile phone electromagnetic field for 48 hours at the state of dormancy, and the second group was exposed to the same electromagnetic field at the state of division. The third group, the control group, was not exposed to an electromagnetic field beyond the natural background. The results obtained in the study indicate that electromagnetic waves emitted from mobile phones affect seeds in the state of dormancy more than the state of germination. Germination rate was not affected under the specified exposure conditions, but root growth decreased due to a possible effect of oxidative stress in the state of dormant seeds. There was also a noticeable increment in the c-mitosis rates, especially in the state of dormant seeds. The reason for this increment could be problems in spindle function

TOTAL SEED STORAGE PROTEIN PATTERNS OF SOME LATHYRUS SPECIES GROWING IN TURKEY USING SDS-PAGE

Present study determined the variation of total electrophoretic seed protein patterns and protein amounts in some Lathyrus species viz., L. tukhtensis, L. cilicicus, L. saxatilis, L. annuus, L. hierosolymitanus, L. gorgoni var. gorgoni, L. Lycicus and L. odoratus collected from their natural habitats of different localities in Turkey. Electrophoretic data were documented by using a gel documentation system (Bio-Rad, USA) and analysed by using Quantity 1-D analysis software and also the dendogram were formed with 4.0% tolerance in UPGAMA (Unweighed Pair-Group Arithmetic Mean). The differences among species were observed and all 9 taxa were clearly identifiable from the protein patterns. The formed dendogram from SDS-PAGE analysis showed that all studied taxa constituted two clusters. The first one consisted of L. saxatilis, L. gorgoni, L. annuus, L. hierosolymitanus, L. lycicus and L. phaselitanus second one by L. tukhtensis, L. cilicicus and L. odoratus. In cluster I, L. saxatilis and L. gorgoni were found to have higher similarity to each other. Also, it was reproted that quantities of total seed proteins in the present study. L. lycicus (79.906 mu g/ml) has highest total protein content whereas L. cilicicus (65.860 mu g/ml) has lowest total protein content

Bronchopulmonary dysplasia and wnt pathway-associated single nucleotide polymorphisms.

AIM: Genetic variants contribute to the pathogenesis of bronchopulmonary dysplasia (BPD). The aim of this study is to evaluate the association of 45 SNPs with BPD susceptibility in a Turkish premature infant cohort. METHODS: Infants with gestational age <32 weeks were included. Patients were divided into BPD or no-BPD groups according to oxygen need at 28 days of life, and stratified according to the severity of BPD. We genotyped 45 SNPs, previously identified as BPD risk factors, in 192 infants. RESULTS: A total of eight SNPs were associated with BPD risk at allele level, two of which (rs4883955 on KLF12 and rs9953270 on CHST9) were also associated at the genotype level. Functional relationship maps suggested an interaction between five of these genes, converging on WNT5A, a member of the WNT pathway known to be implicated in BPD pathogenesis. Dysfunctional CHST9 and KLF12 variants may contribute to BPD pathogenesis through an interaction with WNT5A. CONCLUSIONS: We suggest investigating the role of SNPs on different genes which are in relation with the Wnt pathway in BPD pathogenesis. We identified eight SNPs as risk factors for BPD in this study. In-silico functional maps show an interaction of the genes harboring these SNPs with the WNT pathway, supporting its role in BPD pathogenesis. TRIAL REGISTRATION: NCT03467828. IMPACT: It is known that genetic factors may contribute to the development of BPD in preterm infants. Further studies are required to identify specific genes that play a role in the BPD pathway to evaluate them as a target for therapeutic interventions. Our study shows an association of BPD predisposition with certain polymorphisms on MBL2, NFKBIA, CEP170, MAGI2, and VEGFA genes at allele level and polymorphisms on CHST9 and KLF12 genes at both allele and genotype level. In-silico functional mapping shows a functional relationship of these five genes with WNT5A, suggesting that Wnt pathway disruption may play a role in BPD pathogenesis