23 research outputs found
The role of the viroid central conserved region in cDNA infectivity
International audienc
Identification of multiple structural domains regulating viroid pathogenicity
International audienc
Detection of potato spindle tuber viroid by nucleic acid spot hybridization: Evaluation with tuber sprouts and true potato seed
Testing of Imported Potato Genotypes for Potato Spindle Tuber Viroid with a Tomato-intermediate /Electrophoresis Combined Method
Separation of citrus viroids by shoot-tip grafting in vitro
Arizona 861âS1 citron (Citrus media L.) infected with a severe exocortis isolate containing four citrus viroids was used as source of tissue for shootâtip grafting in vitro. Out of 51 attempts, 25 successful grafts were obtained. Only 16 plants survived transplanting and of these 12 were viroidâfree. The viroid profile of the other four plants showed fewer viroids than the original field source. The significance of these results, as compared with previous reports on the recovery of viroidâfree plants, is discussed. The results show the usefulness of shootâtip grafting in vitro as a tool to recover single viroid isolates from complex field sources