Estabilidade de genótipos de feijoeiro comum com grãos preto no Centro-Sul do Brasil nos anos de 2003/2004.

O objetivo deste trabalho foi identificar genótipos de feijoeiro-comum (Phaseolus vulgaris) tipo preto, com boa adaptabilidade e estabilidade de produção, na Região Centro Sul do Brasil, pelo uso de dois métodos

Commercial quality of black bean genotypes.

Breeding programs are working to supply cultivars with improved agronomical characteristics, such as yield, disease resistance, and upright plant, among others. More recently other characteristics related to commercial quality that could contribute to increase the acceptance of a new cultivar are also being assessed such as market preference, and 100 seed mass; therefore, the objective of this work was to assess the commercial quality of common black bean lines

Commercial quality of carioca common bean genotypes.

The objective of this work was to evaluate the quality of carioca commercial bean genotypes

BRS FC414: cultivar de feijoeiro-comum com alta produtividade e qualidade comercial indicada para cultivo sob pivô central.

BRS FC414 se destaca pela excelente qualidade comercial dos grãos, possuindo arquitetura ereta e moderada resistência à murcha de Fusarium e a podridões radiculares, possibilitando a utilização em áreas de cultivo sob pivô central. 2) De acordo com o desempenho, BRS FC414 é recomendada para as épocas das águas e da seca na região II (Mato Grosso do Sul, Paraná, Santa Catarina, São Paulo e Rio Grande do Sul); das águas, da seca e de inverno na região I (Goiás, Mato Grosso, Tocantins, Maranhão, Bahia, Espírito Santo, Rio de Janeiro e o Distrito Federal); e para a época das águas na região III (Sergipe, Alagoas, Pernambuco, Rio Grande do Norte, Piauí, Ceará e Paraíba)

BRS FC415: cultivar de feijoeiro-comum carioca com alta produtividade, qualidade comercial, escurecimento lento dos grãos e resistência a patógenos de solo.

Nos últimos anos o mercado brasileiro se tornou cada vez mais exigente quanto às características de qualidade comercial dos grãos, como o rendimento de peneira, a massa de 100 grãos e a coloração dos grãos. Durante o período entre a colheita e a comercialização dos grãos tipo carioca ocorre o escurecimento da tonalidade bege do tegumento, reduzindo o valor de mercado, porque a coloração mais escura tem menor remuneração, o que pressiona o agricultor para a comercialização rápida, independentemente do preço de mercado

Cell-free DNA profiling of metastatic prostate cancer reveals microsatellite instability, structural rearrangements and clonal hematopoiesis.

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.BACKGROUND: There are multiple existing and emerging therapeutic avenues for metastatic prostate cancer, with a common denominator, which is the need for predictive biomarkers. Circulating tumor DNA (ctDNA) has the potential to cost-efficiently accelerate precision medicine trials to improve clinical efficacy and diminish costs and toxicity. However, comprehensive ctDNA profiling in metastatic prostate cancer to date has been limited. METHODS: A combination of targeted and low-pass whole genome sequencing was performed on plasma cell-free DNA and matched white blood cell germline DNA in 364 blood samples from 217 metastatic prostate cancer patients. RESULTS: ctDNA was detected in 85.9% of baseline samples, correlated to line of therapy and was mirrored by circulating tumor cell enumeration of synchronous blood samples. Comprehensive profiling of the androgen receptor (AR) revealed a continuous increase in the fraction of patients with intra-AR structural variation, from 15.4% during first-line metastatic castration-resistant prostate cancer therapy to 45.2% in fourth line, indicating a continuous evolution of AR during the course of the disease. Patients displayed frequent alterations in DNA repair deficiency genes (18.0%). Additionally, the microsatellite instability phenotype was identified in 3.81% of eligible samples (≥ 0.1 ctDNA fraction). Sequencing of non-repetitive intronic and exonic regions of PTEN, RB1, and TP53 detected biallelic inactivation in 47.5%, 20.3%, and 44.1% of samples with ≥ 0.2 ctDNA fraction, respectively. Only one patient carried a clonal high-impact variant without a detectable second hit. Intronic high-impact structural variation was twice as common as exonic mutations in PTEN and RB1. Finally, 14.6% of patients presented false positive variants due to clonal hematopoiesis, commonly ignored in commercially available assays. CONCLUSIONS: ctDNA profiles appear to mirror the genomic landscape of metastatic prostate cancer tissue and may cost-efficiently provide somatic information in clinical trials designed to identify predictive biomarkers. However, intronic sequencing of the interrogated tumor suppressors challenges the ubiquitous focus on coding regions and is vital, together with profiling of synchronous white blood cells, to minimize erroneous assignments which in turn may confound results and impede true associations in clinical trials.The Belgian Foundation Against Cancer (grant number C/2014/227); Kom op tegen Kanker (Stand up to Cancer), the Flemish Cancer Society (grant number 00000000116000000206); Royal College of Surgeons/Cancer Research UK (C19198/A1533); The Cancer Research Funds of Radiumhemmet, through the PCM program at KI (grant number 163012); The Erling-Persson family foundation (grant number 4-2689-2016); the Swedish Research Council (grant number K2010-70X-20430-04-3), and the Swedish Cancer Foundation (grant number 09-0677)

Relatório de estágio em farmácia comunitária

Relatório de estágio realizado no âmbito do Mestrado Integrado em Ciências Farmacêuticas, apresentado à Faculdade de Farmácia da Universidade de Coimbr

Cooking time of black beans genotypes evaluated in different environments.

Lines developed by breeding programs are expected to have improved agronomical characteristics, good culinary quality such as environment affected cooking time. Since the final quality tests of black bean lines from the Rice and Beans Research Center breeding program are carried out in a great number of environments. it is possible to determine the cooking time of lines tested in those environments and to verify the presence of genotype x ambient interaction