KADAR MALONDIALDEHID DAN AKTIVITAS ENZIM SUPEROKSIDA DISMUTASE PADA HIPERTENSI DAN NORMOTENSI

Hypertension is a condition of high blood pressure caused by many factors. One of them is endothelial dysfunction indicated from malondialdehyde (MDA) level. The production of MDA is primarily prevented by endogenous antioxidant such as superoxide dismutase (SOD). This study aimed to identify the differences between level of MDA and the activity of SOD in hypertensive and normotensive. It also aimed to identify the correlation between MDA level and SOD activity in both group. This study was conducted in laboratory of animal physiology FMIPA UNJ on June to September 2015. This research used an Ex-Post facto method with a cross-sectional design. A total of 30 serum samples (N1 = N2 = 15) was examined to identify MDA level and SOD activity. Data of MDA level and SOD activity was analyzed by Mann-Whitney test and t-test, respectively. Correlation between MDA level and SOD activity was analyzed by Pearson Correlation test in normotensive group and by Spearman’s Rho test in hypertensive group. Based on the results, mean value MDA level in normotensive was 0,465±0,118 nmol/mL and in hypertensive is 1,279±0,307 nmol/mL. MDA level in normotensive was significantly different than hypertensive (Sig.=0,000). Mean value SOD activity in normotensive was 2,307±0,739 u/mL, and in hypertensive was 2,131±0,422 u/mL. SOD activity was not significantly different in normotensive and hypertensive (Sig.=0,432). In conclusion, there was no correlation between MDA level and SOD activity in hypertensive and normotensive group

Follicular Development of Aged Rats Ovarian Injected Human Umbilical Cord Mesenchymal Stem Cells

Female reproductive system showing the fastest signs of aging. The ovarian aging characterized by a decrease in follicular development. Stem cells are undifferentiated cells and can form a variety of different cells as the foundation of tissues and organs. Previous studies reported that Bone Marrow Mesenchymal Stem Cells (BM-MSCs) transplantation can restore follicular development in damaged ovarian rats. This study aimed to analyze the number of follicular development in aged rats and to analyze the capability of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) to improving follicular development in aged rats. This study used 3 mature rats (4 months old), and 9 nine aged rats (22-24 months old), Spraque Dawley (SD) strain. They were divided into four groups. The first and the second group was mature rats and aged rats without injection. The third and the fourth group was aged rats injected hUC-MSCs dose 106 cells/kgBW and hUC-MSCs dose 107 cells/kgBW. The injection carried out 4 times at 3-month intervals. The parameters observed were follicular development and homing image of hUC-MSCs in ovarian tissue. The results showed that the number of follicular developments in aged rats 22-24 months decreased significantly compared to mature rats 4 months old. Injection of hUC-MSCs at dose 106 cells/kgBW and 107 cells/kgBW did not increase follicular development in aged rats. hUC-MSCs did not found in ovarian tissue. It could be concluded that aged rats 22-24 months old no longer productive indicated from the number of follicular developments and corpus luteum decreased. The injection of hUC-MSCs intravenously did not indicate an improvement of follicular development in aged rats 22-24 months old