bFGF decreased α-SMA and TGF-β1 expression in scars.

<p>Immunohistochemistry of the expression of (A) α-SMA and (B) TGF-β1 in scars treated with saline or bFGF in scars. (C) The levels of α-SMA and TGF-β1 in scars treated with saline or bFGF by Western blot. Analysis of the relative protein of (E) α-SMA and (F) TGF-β1 was performed. **<i>P</i><0.01 compared to control group.</p

bFGF alleviated the scar formation in rabbit ear model.

<p>(A) The averaged Epidermal Thickness Index (ETI) of the scars. Epidermal hypertrophy was displayed by ETI. ETI >1 depicts a hypertrophic epidermis. (B) The averaged scar elevation index (SEI) of the scars. Dermal hypertrophy is displayed by the SEI, where SEI >1 depicts a hypertrophic scar. *<i>P</i><0.05, **<i>P</i><0.01 compared to control group, n = 6. (C) The microscopic histology of wounds that control or bFGF at day 40, HE stain.</p

The expression of PCNA, CD68 and TGF-β1 after bFGF treatment.

<p>Immunohistochemistry of (A) PCNA, (B) CD68 and (C) TGF-β1 was performed on the indicated day (×200); the histogram represents the positive cells and optical density of the immunohistochemistry results. *<i>P</i><0.05 and **<i>P</i><0.01 compared to control group, n = 8.</p

Wound closure and histopathological characteristics of bFGF treated wound healing in rat.

<p>(A) Representative photographs of full-thickness skin wounds at various time points after treatment with or without 1 µg/ml bFGF. (B) The wound healing rates of bFGF. *<i>P</i><0.05 compared to control group, n = 8. (C) Histopathological observation and masson staining of collagen in wound healing at day 14 post-wounding (×200).</p

bFGF decreased collagen I and collagen III synthesis.

<p>The levels of expression of (A) collagen Ι and (B) collagen III in scars treated with saline or bFGF, *<i>P</i><0.05, **<i>P</i><0.01 compared to control group, n = 6. (C) Immunohistochemistry of the expression of collagen III in scars treated with saline or bFGF (×200). (D) Analysis of relative density collagen III, *<i>P</i><0.05, **<i>P</i><0.01 compared to control group, n = 6.</p

Effects of bFGF on mRNA levels in HSF.

<p>RT-PCR analysis the mRNA levels of type collagen Ι and (B) collagen III, (C) CTGF, (D) HGF, (E) FN, (F) MMP-1, (G) TIMP-1, (H) SMAD-2, (I) SMAD-7, (J) α-SMA, (K) Lysine hydroxylases and (L) Prolyo hydroxylase in HSF treated with bFGF or saline for 5 days. *<i>P</i><0.05 compared to control group.</p

Primers used for real-time RT-PCR analysis.

<p>Primers used for real-time RT-PCR analysis.</p