PENATALAKSANAAN KARIES RAMPAN DAN MEMPERBAIKI ESTETIS DENGAN PEMAKAIAN GIGI TIRU ANAKRILIK SEBAGIAN LEPASAN PADA ANAK USIA 4 TAHUN

Kariesrampan merupakan bentuk kerusakan gigi yang parah pada gigi sulung maupun gigi permanen dengan karakteristik onset dan progresifitas yang sangatcepat .Lesiawalakan segera tampak pada permukaan labialinsisif atas sesaat setelah erups igigi. Etiologi kariesrampan sangat kompleks dan multifaktorial serta sangat berhubungan dengan berbagai faktorrisiko. Perawatan kariesrampan harus dilakukan secara menyeluruh, meliputi tindakan menghilangkan rasa sakit, pencegahan, dan perawatan kuratif. Tujuan makalah ini untuk melaporkan penatalaksanaan kasus kariesrampan dan Memperbaiki estesis dengan pemakaian gigi tiruana krilik sebagian lepasan pada anak perempuan usia 4 tahun yang kehilangan beberapa gigi sulung akibat pencabutan. pemasangan gigi tiruan sebagian lepasan pada pasien ini mampu memperbaiki fungsi pengunyahan serta estetik pada pasien

Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and Viscosity

Objective: To analyze the ability of saliva in controlling the growth and the biofilm formation of Streptococcus mutans (S. mutans) as well as the effect of histatin-5 anti-biofilm relate to pH and saliva viscosity. Material and Methods: The S. mutans biofilm assayed by crystal violet 1% and its growth measured by spectrophotometer. The saliva viscosity was analyzed by viscometer, and pH of saliva was measured by pH meter. Results: Based on the optical density values, growth of S. mutans in saliva ranged <300 CFU/mL (0.1 nm) at concentrations of 25%, 12.5% and 6.25% for 24 hours. Whereas at the 48 h and 72 h period of incubation shown an increase in growth of S. mutans ranged 300-600 CFU/mL (0.2-0.36 nm). The inhibitory biofilm formation of S. mutans in saliva was significantly higher at concentrations of 12.5% and 6.25% at 24 h incubation times on a moderate scale, whereas the histatin-5 was effective to inhibit S. mutans biofilm on the 50 and 25 ppm. The saliva possessed a higher inhibitory of biofilm S. mutans than histatin-5 and good level viscosity (0.91-0.92 cP). Conclusion: The saliva was able to control the growth of S. mutans, and histatin-5 can inhibit the biofilm formation S. mutans. Furthermore, the saliva was also able to respond to the pH change with good viscosity of saliva

Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and Viscosity

Objective: To analyze the ability of saliva in controlling the growth and the biofilm formation of Streptococcus mutans (S. mutans) as well as the effect of histatin-5 anti-biofilm relate to pH and saliva viscosity. Material and Methods: The S. mutans biofilm assayed by crystal violet 1% and its growth measured by spectrophotometer. The saliva viscosity was analyzed by viscometer, and pH of saliva was measured by pH meter. Results: Based on the optical density values, growth of S. mutans in saliva ranged <300 CFU/mL (0.1 nm) at concentrations of 25%, 12.5% and 6.25% for 24 hours. Whereas at the 48 h and 72 h period of incubation shown an increase in growth of S. mutans ranged 300-600 CFU/mL (0.2-0.36 nm). The inhibitory biofilm formation of S. mutans in saliva was significantly higher at concentrations of 12.5% and 6.25% at 24 h incubation times on a moderate scale, whereas the histatin-5 was effective to inhibit S. mutans biofilm on the 50 and 25 ppm. The saliva possessed a higher inhibitory of biofilm S. mutans than histatin-5 and good level viscosity (0.91-0.92 cP). Conclusion: The saliva was able to control the growth of S. mutans, and histatin-5 can inhibit the biofilm formation S. mutans. Furthermore, the saliva was also able to respond to the pH change with good viscosity of saliva

Characteristics and Antibacterial Properties of Film Membrane of Chitosan-Resveratrol for Wound Dressing

The research aimed to evaluate the film membrane of Nano Chitosan Resveratrol (NCHR) for biological, physicochemical, and antibacterial properties. Psychochemically, the functional groups of chitosan compounds were examined by FTIR, chemical compounds by GCMS, and the morphology of chitosan and chemical elements by SEM-EDS. Biologically, the characteristics of NCHR were examined by solubility, swelling, permeability, and biodegradation tests. Meanwhile, the antibacterial properties were examined for inhibition of Porphyromonas gingivalis (P. gingivalis) ATCC 33277 by Minimal inhibition concentration (MIC) and growth assessment by spectrophotometry. Nano Chitosan (NCH) has appeared at 1033.85 cm-1 as a sharp peak indicating the P=O group and contains anti-toxicity compounds (Ethane, 1,1-diethoxy- (CAS) 1,1-Diethoxye) is 81.06% and antioxidant compounds Limonene is (1.28%). In addition, NCH has chemical elements, Oxygen Weight (69.4%), calcium (19.7%), magnesium (6.6%), and phosphorus (4.3%). NaCl 0.9%, PBS, and Aquades. In addition, it has an excellent index of water vapor transmission rate (WVTR) in all solvents (R2³ 0.95). The NCHR membrane film is bacteriostatic (≤ 300 CFU/mL) with each value of Minimal Inhibition Concentration (MIC) >15 mm. The Nano chitosan contains antitoxic, antioxidant, and antibacterial compounds with high oxygen elements. The film membrane of nano chitosan resveratrol can maintain the stability of changes in pH with a very high solubility index, swelling index, and WVTR index, as well as good biodegradation and antibacterial properties. Doi: 10.28991/ESJ-2023-07-03-012 Full Text: PD

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Evaluation of a membrane filter and platelet-rich plasma (PRP) product obtained from a prototype PRP kit that works through a new method for separating PRP based on cell dimensions

The harvesting of platelet-rich plasma (PRP) from whole blood based on cell density is a standard procedure that is currently applied to commercially available PRP kits. Leukocytes and erythrocytes, which are closer in density, contaminate a significant amount of PRP products, mostly commercial PRP kits. In this study, we tested membrane filters and PRP products from our prototype PRP kit. We did this by putting a membrane filter with pores of 2 μm in the middle of the tube, which is a new way to separate things based on the cell dimension method (CDM). The evaluations were performed for membrane filter use, hematology analysis, blood smears, viability and cytotoxicity assays, and fibrin structure by scanning electron microscopy (SEM). Compared to the density method (DM), the CDM enables the elimination of a significant number of leukocytes and erythrocytes from the PRPs (CDM-PRP) and a significant increase in the number of platelets compared to the whole blood and DM-PRP. Furthermore, both DM-PRP and CDM-PRP increased the cell viability in L929 cells by adding them at 5% in the culture medium. In addition to CDM-PRP having the lowest cytotoxicity based on the LDH assay, the fibrin structure of CDM-PRP blood clots is characterized by thickness and firmness with a network structure. Thus, we believe that the PRP from the prototype PRP kit meets the requirements as a biomaterial for medical treatments