Anti-Urolithiatic Activity of Cassia Auriculata Ethanolic Seed Extract in Wistar Rats

Background: Urolithiasis is a medical condition that, despite substantial research in the field of urology, has yet to find a cure within the allopathic medical approach. The process of stone development, known as nephrolithiasis, can occur within the kidney or any segment of the urinary tract, encompassing the ureters and bladder. Material and Methods: The seeds of Cassia auriculata (Linn.) were obtained in June 2021 from Mettukadai hamlet, located in the Erode District of Tamilnadu, India. The herbarium of the plant was meticulously assembled, verified, and afterwards deposited as a voucher specimen. The voucher specimen was retained within the college premises for future reference. Results: The current investigation involved conducting a preliminary phytochemical analysis on the ethanolic seed extract of Cassia auriculata Linn. The research revealed the existence of many phytochemical constituents, including Alkaloids, Flavanoids, Carbohydrate, Sterols, Phytosterols, Phenols, Terpenoids, Amino acids, and Anthraquinones. The acute toxicity experiments revealed that rats exhibited tolerance to a maximum dose of 2000 mg/kg body weight, and no discernible alterations in behavior were detected across all experimental groups. Hence, fractions equivalent to one-fourth and one-eighth of the maximum tolerated dose of 200 mg/kg body weight were selected for subsequent investigations. Conclusion: In summary, the findings suggest that the application of an ethanolic seed extract derived from Cassia auriculata Linn effectively decreased the progression of urinary stone formation. Additionally, it appears that the efficacy of the treatment impact surpasses that of its preventative counterpart

Screening and characterization of high lipid accumulating microalga Ankistrodesmus sp. from freshwater environment

931-936Microalgae based biofuel is an attractive alternative energy source due to its rapid growth rate and high lipid accumulation efficiency. In this study, we screened high lipid content microalga with the favourable fatty acid composition suitable for biodiesel production. Totally twelve different microalgal species were isolated from freshwater habitats. The isolates were identified as Micractinium sp., Chlorella sorokiniana, Scenedesmus bajacalifornicus, Desmodesmus sp., Scenedesmus obliquus, Coelastrum proboscideum, Chlamydomonas moewusii, Chlamydomonas debaryana, Chlamydomonas dorsoventralis, Coelastrum sp., and Ankistrodesmus sp. based on morphological features and ITS region similarity. Among the isolates, highest lipid content (33±0.07%) and lipid productivity (0.27±0.06 g L-1) were obtained from Ankistrodesmus sp. Intracellular lipid droplets of Ankistrodesmus sp. were observed through Nile red staining. The lipid content was enhanced up to 45% under the nitrogen deficient (5 mg L-1) BG-11 medium. Ankistrodesmus sp. fatty acid profile shows the presence of palmitic (16.39%), stearic (15.67%), oleic (25.66%), linolenic (21.62%), and alpha-linoleic acids (14.34%). The oleic acid was the dominant fatty acid 25.66% in the nitrogen deficient condition