15 research outputs found
Mapping of Quantitative Trait Loci for Grain Iron and Zinc Concentration in Diploid A Genome Wheat
Micronutrients, especially iron (Fe) and zinc (Zn), are deficient in the diets of people in underdeveloped countries. Biofortification of food crops is the best approach for alleviating the micronutrient deficiencies. Identification of germplasm with high grain Fe and Zn and understanding the genetic basis of their accumulation are the prerequisites for manipulation of these micronutrients. Some wild relatives of wheat were found to have higher grain Fe and Zn concentrations compared with the cultivated bread wheat germplasm. One accession of Triticum boeoticum (pau5088) that had relatively higher grain Fe and Zn was crossed with Triticum monococcum (pau14087), and a recombinant inbred line (RIL) population generated from this cross was grown at 2 locations over 2 years. The grains of the RIL population were evaluated for Fe and Zn concentration using atomic absorption spectrophotometer. The grain Fe and Zn concentrations in the RIL population ranged from 17.8 to 69.7 and 19.9 to 64.2 mg/kg, respectively. A linkage map available for the population was used for mapping quantitative trait loci (QTL) for grain Fe and Zn accumulation. The QTL analysis led to identification of 2 QTL for grain Fe on chromosomes 2A and 7A and 1 QTL for grain Zn on chromosome 7A. The grain Fe QTL were mapped in marker interval Xwmc382-Xbarc124 and Xgwm473-Xbarc29, respectively, each explaining 12.6% and 11.7% of the total phenotypic variation and were designated as QFe.pau-2A and QFe.pau-7A. The QTL for grain Zn, which mapped in marker interval Xcfd31-Xcfa2049, was designated as QZn.pau-7A and explained 18.8% of the total phenotypic variatio
Nutrigenomics: advances, opportunities and challenges in understanding the nutrient-gene interactions
Background: Nutrients and bioactive compounds present in the foods are known to interact with genes to alter their expression, which consequently results in several chronic and diet-associated disease conditions
Food waste: a potential bioresource for extraction of nutraceuticals and bioactive compounds
Abstract Food waste, a by-product of various industrial, agricultural, household and other food sector activities, is rising continuously due to increase in such activities. Various studies have indicated that different kind of food wastes obtained from fruits, vegetables, cereal and other food processing industries can be used as potential source of bioactive compounds and nutraceuticals which has significant application in treating various ailments. Different secondary metabolites, minerals and vitamins have been extracted from food waste, using various extraction approaches. In the next few years these approaches could provide an innovative approach to increase the production of specific compounds for use as nutraceuticals or as ingredients in the design of functional foods. In this review a comprehensive study of various techniques for extraction of bioactive components citing successful research work have been discussed. Further, their efficient utilization in development of nutraceutical products, health benefits, bioprocess development and value addition of food waste resources has also been discussed
Effect of gibberellins and ascorbic acid treatment on phytic acid and micronutrients dialyzability in germinated biofortified wheat seeds
Introduction: Phytic acid chelate minerals, including Fe and Zn and render them inaccessible once ingested by human beings. The evaluation of differences in the dialyzability of macronutrients including Fe and Zn in various wheat derivatives is therefore important for the enhancement of nutritional quality of grains. Objectives: The objective of current study was to improve the micronutrient content in wheat grain. Methods: During germination (12, 24, 48, 72, 96 h), effects of gibberellins and ascorbic acid on phytic acid content as well as dialyzability of iron and zinc of wheat derivatives were determined. Results: The phytic acid content in wheat flour was determined and it was found 7.61 to 7.48 mg/g. After the treatment with gibberellins, it was significantly reduced from 8.68 to 21.6 % and 9.65 to 20.9 % with ascorbic acid. In wheat flour dialyzabilty of Fe was 4.53 to 8.97 mg/kg. After germination, Fe content was increased from 9.77 to 32.0 % with gibberellin, and 13.9 to 31.0 % with ascorbic acid. Moreover, with gibberellins, Zn content was increased from 8.68 to 21.6 % and 9.65 to 20.9 % with ascorbic acid, respectively. Conclusion: These results suggested that gibberellins as well as ascorbic acid can be exploited to improve the dialyzability of iron and zinc content due to reduced antinutrient i. e phytic acid and make the minerals available for the absorption in monogastric animals including human beings
Uptake, distribution, and remobilization of iron and zinc among various tissues of wheat–Aegilops substitution lines at different growth stages
Biofortification of wheat for higher grain iron and zinc is the most feasible and cost-effective approach for alleviating micronutrient deficiency. The non-progenitor donor Aegilops species had 2–3 times higher grain iron and zinc content than the wheat cultivars, whereas the wheat–Aegilops substitution lines mostly of group 2 and 7 chromosomes had intermediate levels of grain micronutrients. The non-progenitor Aegilops species also had the highest iron content and intermediate-to-highest zinc content in straw, lower leaves, and flag leaves at the pre-anthesis, grain-filling, and maturity growth stages. The micronutrients accumulation status is followed by wheat–Aegilops substitution lines and is the least in wheat cultivars indicating that the donor Aegilops species and their substituted chromosomes possess genes for higher iron and zinc uptake and mobilization. The grain iron content was highly positively correlated with iron content in the plant tissues. Most of the lines had much higher iron and zinc content in all tissues during grain-filling period indicating higher iron and zinc uptake from soil during this stage. Although iron and zinc contents are nearly similar in grains, there was much less zinc content in the plant tissues of all the lines suggesting that the Triticeae species take up less zinc which is mobilized to grains more effectively than iron
Transferability and Polymorphism Between Group 7 Chromosome Specific Simple Sequence Repeat (SSR) Markers of Bread Wheat and Its Related Non-Progenitor <i>Aegilops</i> Species
<p>Simple sequence repeat (SSR) markers are highly efficient for genetic mapping and molecular breeding in crop plants. Chromosomes 7S and 7U of related non-progenitor <i>Aegilops</i> species possess superior genetic systems for high grain iron and zinc content. This study was undertaken to conduct a comprehensive analysis of transferability and polymorphism among group-7 anchored SSR markers of bread wheat (<i>Triticum aestivum</i> L.) to <i>Aegilops</i> species for wheat improvement. The study revealed 77% transferability of group 7-specific SSR markers of bread wheat to 7U/7S chromosomes of <i>Aegilops</i> species. More than 80% of the 7D specific markers were found to be transferable with a high level of polymorphism. The transferability to 7S and 7U genome suggested higher similarity between bread wheat genome(s) and S genome as compared with U genome of <i>Aegilops</i> species. These polymorphic markers are highly informative, robust and cost-effective for molecular breeding and could be further utilized for identification of introgression/transfer(s) of 7S/7U chromosomal fragments in bread wheat genome through wide hybridization.</p