Fund for Shared Insight: Media Analysis

Fund for Shared Insight ("Shared Insight") is a collaborative effort among fundersthat pools financial and other resources to make grants to improve philanthropy. Shared Insight believes philanthropy can have a greater social and environmental impact if foundations and nonprofits listen to the people they seek to help, act on what they hear, and openly share what they learn.Related to feedback loops, Shared Insight's work is focused on increasingthe extent to which foundations listen to others—especially the people they seek to help—and respond to their expressed interests. When Shared Insight talks about "the people they seek to help," they are referring to the individuals who receive programs and services from nonprofit organizations; for example, the students served by charter schools, the recently released prisoners benefiting from job-training services, and the low-income first-time mothers participating in prenatal through birth programs.Over the next three years, Shared Insight would hope to see changes in the amount and kind of discourse in the field related tobeneficiary feedback loops. In the summer of 2015, one year since the launch of the collaborative, ORS Impact repeated a media analysis of relevant blogs, periodicals, and reports. The following memo outlines changes in the amount and kind of discourse in the field around feedback loops compared to the year before Shared Insight launched. We raise a few observations and considerations. More detailed methodological notes follow

Case report: Fully endoscopic microvascular decompression for glossopharyngeal neuralgia

With the advances in endoscopic technology, endoscopy is widely used in many neurosurgical procedures, such as microvascular decompression, which is an effective method to treat glossopharyngeal neuralgia, trigeminal neuralgia, and facial spasm. The purpose of this study was to determine the efficacy of fully endoscopic microvascular decompression in the treatment of glossopharyngeal neuralgia. We managed a patient with glossopharyngeal neuralgia in our department, whose main clinical manifestation was recurrent left ear and facial pain for 3 years. The patient underwent a fully endoscopic microvascular decompression. The pain in the left ear and face was significantly relieved postoperatively, and there was no recurrence at the 6-month follow-up evaluation. We describe a case of glossopharyngeal neuralgia that was successfully treated by fully endoscopic microvascular decompression, which showed that endoscopy has advantages in microvascular decompression, and fully endoscopic microvascular decompression is an effective method for glossopharyngeal neuralgia

New insights into the germline genes and CDR3 repertoire of the TCRβ chain in Chiroptera

IntroductionBats are recognized as natural reservoirs for many viruses, and their unique immune system enables them to coexist with these viruses without frequently exhibiting disease symptoms. However, the current understanding of the bat adaptive immune system is limited due to the lack of a database or tool capable of processing T-cell receptor (TCR) sequences for bats.MethodsWe performed germline gene annotation in three bat species using homologous genes and RSSs (Recombinational Signal Sequences) scanning method. Then we used the conserved C gene to construct the TCRβ chain receptor library of the Intermediate Horseshoe Bat. Bats' TCRβ data will be analyzed using MiXCR and constructed reference library.ResultsRegarding the annotation results, we found that the Pale Spear-nosed Bat has 37 members in the TRBV12 family, which is more than the total number of TRBV genes in the Greater Horseshoe Bat. The average number of unique TCRβ chain receptor sequences in each Intermediate Horseshoe Bat sample reached 24,904.DiscussionThe distinct variations in the distribution of TRBV genes among the three types of bats could have a direct impact on the diversity of the TCR repertoire, as evidenced by the presence of conserved amino acids that indicate the T-cell recognition of antigens in bats is MHC-restricted. The bats’ TCRβ repertoire is formed through the rearrangement of the V-D-J-C genes, with D-J/V-D deletions and insertions resulting in high diversity

Endoscopic endonasal surgery for non-invasive pituitary neuroendocrinology tumors with incomplete pseudocapsule

BackgroundPituitary neuroendocrinology tumors (PitNETs) with pseudocapsule can be effectively removed by the pseudocapsule-based extracapsular resection technique. In the areas without pseudocapsule, the tumor cells can spread into the adjacent tissues at the cellular level, which brings a great challenge to achieving total tumor resection.MethodsOur surgical strategy for PitNETs with an incomplete pseudocapsule is to combine the pseudocapsule-based extracapsular resection technique with the intensive excision technique for the removal of the tumor. Specifically, the pseudocapsule-based extracapsular resection technique is applied in the areas with pseudocapsule, while in the areas without pseudocapsule, the intensive excision technique bounded by adjacent normal structures is adopted. Moreover, a pathological examination was performed to determine the situations of pseudocapsule and tumor cell remnant.ResultsAll growth hormone-secreting PitNETs achieved biochemical remission after surgery. There was no deterioration of pituitary functions postoperatively, and the preoperative hypopituitarism had improved in all patients postoperatively. In total, two cases suffered a transient diabetes insipidus, and intraoperative cerebrospinal fluid leakage was observed in two cases but no postoperative cerebrospinal fluid leakage in all cases. There was no recurrence during the follow-up. The fragmental pseudocapsule and small tumor remnants were found in the majority of suspicious tissues by histological staining.ConclusionThe effectiveness and safety of the surgical strategy were preliminarily explored for removing PitNETs without incomplete pseudocapsules. In overview, the pseudocapsule-based extracapsular resection technique is applied in areas with pseudocapsule, while the intensive excision bounded by adjacent normal structures is adopted in other areas

Responsed Letter and Raw data (1# and 2# documents)

Responsed letter 1# document Supplementary figure 6J (original pictures, tiff) 2# document Raw data of this article (including WB and IF images, data for statistics)  </p

Related clinical data for Figure 6F-G

Related clinical data for Figure 6F-G .To view the data, use the iViewer software to open it</p

pancancer mRNA data and panGTEX mRNA data

pancancer mRNA data for Figure 6K and panGTEX mRNA data for Figure S6L. Use the "correlation analysis code.R" file to open it. </p

An Experimental Study of 3D Electrode-Facilitated Particle Traffic Flow-Focusing Driven by Induced-Charge Electroosmosis

In this paper we present a novel microfluidic approach for continuous, rapid and switchable particle concentration, using induced-charge electroosmosis (ICEO) in 3D electrode layouts. Field-effect control on non-linear electroosmosis in the transverse direction greatly facilitates a selective concentration of biological yeast cells from a straight main microchannel into one of the three downstream branch channels in our microfluidic device. For the geometry configuration of 3D driving electrode plates on sidewalls and a 2D planar gate electrode strip on the channel bottom surface, we briefly describe the underlying physics of an ICEO-based particle flow-focusing method, and provide relevant simulation results to show how gate voltage amplitude can be used to guide the motion trajectory of the concentrated particle stream. With a relatively simple geometrical configuration, the proposed microfluidic device provides new possibilities to controllably concentrate micro/nanoparticles in continuous flow by using ICEO, and is suitable for a high-throughput front-end cell concentrator interfacing with various downstream biosensors