Evaluation of chemical compounds for induction of male sterility in wheat (Triticum aestivum L.)

The short-list of eleven chemical hybridizing agents (CHAs) showing 98% or more induction of male sterility were identified following application at the pre-meiotic stage of wheat. Among ethyl oxanilates, 4-fluoro (CHA A1), 4-bromo (CHA A2), 4-trifluoromethyl (CHA A5), and 4-cyano (CHA A3) derivatives; and among pyridones, 4-chloro (CHA B3), 4-fluoro (CHA B1), 4-bromo (CHA B2), and 4-trifluoromethyl (CHA B6) derivatives were the most promising. These agents showed no adverse effects on plant growth and yield. Ethyl 4-fluoro oxanilate (CHA A1) was tested on 29 wheat genotypes at 1500 ppm and induced 99.76±0.37% male sterility. Ethyl 4-fluoro oxanilate residues were non-detectable in grain and husk and thus appeared to have no lasting residue effects

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF ESCITALOPRAM OXALATE AND FLUPENTIXOL DIHYDROCHLORIDE IN COMBINED DOSAGE FORM AND PLASMA

Objective: The objective of the study was to develop a simple and rapid chromatographic method for quantification of escitalopram oxalate and flupentixol dihydrochloride in combined dosage form and plasma. Methods: The separation was achieved with a sun fire C8 [150×4.6 mm] 3.5 µm column with an isocratic mobile phase containing a mixture of potassium dihydrogen orthophosphate buffer: methanol: acetonitrile [30:60:10 v/v/v] pH adjusted to 11. The flow rate of the mobile phase was 1.5 ml/min with a Photodiode array [PDA] detection at 230 nm. Results: The HPLC method was developed and validated with respective linearity, accuracy, and precision, detection of limit, robustness, and specificity. The precision of the results stated as the relative standard deviation was below 2 %. The calibration curve was linear over a concentration range from 10-50 µg/ml for escitalopram oxalate and 1-5 µg/ml for flupentixol dihydrochloride with a correlation co-efficient 0.994 and 0.977 respectively. The accuracy of the method was demonstrated at levels in the range of 100 % and 120 % of the specification limit. The recovery of escitalopram oxalate and flupentixol dihydrochloride was found to be in the range of 90 % to 88 %, respectively. The lowest detection limits were found to be 2 µg/ml for escitalopram oxalate and 0.1 µg/ml for flupentixol dihydrochloride. The lowest quantification limits were found to be 5 µg/ml of escitalopram oxalate and 0.5 µg/ml of flupentixol dihydrochloride. Conclusion: The developed method was validated for linearity, accuracy, precision, the limit of detection and quantification, specificity. The method was applied successfully for the determination of escitalopram oxalate and flupentixol dihydrochloride in the combined dosage form and plasma

Adaptive undivided wheel opener

Adaptive undivided wheel opener is a special purpose tool used to tighten and loosen all the nuts of wheel at the same time with less effort. It uses bevel gear assembly for transmitting torque to the nuts and lock – nut arrangement have been used to make the system adjustable to the required pitch length. The most desired advantage is that the total time consumed in the process is very less. It can open and refit the wheel with same tool. Tool is simple in design and easily portable along with vehicle

The role of advocacy in promoting better child health

Child health professionals play a crucial role in advocating for children and it is incumbent on all who work with them to observe and enforce the maintenance of children's rights. In this article we summarise what advocacy is, its importance for child health and briefly how to go about it. Finally, we provide three differing examples of how advocacy has been used to improve the health of children

Extraction of Protein Interaction Data: A Comparative Analysis of Methods in Use

Several natural language processing tools, both commercial and freely available, are used to extract protein interactions from publications. Methods used by these tools include pattern matching to dynamic programming with individual recall and precision rates. A methodical survey of these tools, keeping in mind the minimum interaction information a researcher would need, in comparison to manual analysis has not been carried out. We compared data generated using some of the selected NLP tools with manually curated protein interaction data (PathArt and IMaps) to comparatively determine the recall and precision rate. The rates were found to be lower than the published scores when a normalized definition for interaction is considered. Each data point captured wrongly or not picked up by the tool was analyzed. Our evaluation brings forth critical failures of NLP tools and provides pointers for the development of an ideal NLP tool

IDENTIFICATION OF BIOACTIVE COMPOUNDS BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF SYZYGIUM JAMBOS (L.) COLLECTED FROM WESTERN GHATS REGION COIMBATORE, TAMIL NADU

Objective: The aim of this study was to investigate the presence of bioactive compounds in the methanolic leaf extract of Syzygium jambos.Methods: Collected leaves were shade dried and made into fine powder, extracted with methanol, and the methanolic extract was prepared and analyzed for the presence of bioactive compounds by gas chromatography-mass spectrometry (GC-MS). The mass spectrum of the chromatography was matched with NIST and WILEY Libraries.Results: The GC-MS analysis revealed the presence of 45 active compounds in the extract. From the GC-MS investigation, 1-Deoxy-d-mannitol3-methyl-2-methylsulfanyl-5-nitro-6-pyridin-4-ylpyrimidin-4-one, 3-Pentadecylphenol, 2-biphenylene carboxylic acid, Quinoline-3-carboxylic acid, and Stigmast-5-en-3-ol are important phytoconstituents which have antipyretic and antiparasitic activities.Conclusion: The present investigation revealed preliminary information on phytocompounds presented in S. jambos leaf extract which is very useful for the human community.Keywords: Syzygium jambos, Gas chromatography-mass spectrometry analysis, 1-Deoxy-d-mannitol, Phytoconstituents, Methanolic leaf extrac