A Neural Circuit Model for Prospective Control of Interceptive Reaching

Two prospective controllers of hand movements in catching -- both based on required velocity control -- were simulated. Under certain conditions, this required velocity controlled to overshoots of the future interception point. These overshoots were absent in pertinent experiments. To remedy this shortcoming, the required velocity model was reformulated in terms of a neural network, the Vector Integration To Endpoint model, to create a Required Velocity Integration To Endpoint modeL Addition of a parallel relative velocity channel, resulting in the Relative and Required Velocity Integration To Endpoint model, provided a better account for the experimentally observed kinematics than the existing, purely behavioral models. Simulations of reaching to intercept decelerating and accelerating objects in the presence of background motion were performed to make distinct predictions for future experiments.Vrije Universiteit (Gerrit-Jan van Jngen-Schenau stipend of the Faculty of Human Movement Sciences); Royal Netherlands Academy of Arts and Sciences; Defense Advanced Research Projects Agency and Office of Naval Research (N00014-95-1-0409

The role of areas MT+/V5 and SPOC in spatial and temporal control of manual interception: an rTMS study

Manual interception, such as catching or hitting an approaching ball, requires the hand to contact a moving object at the right location and at the right time. Many studies have examined the neural mechanisms underlying the spatial aspects of goal-directed reaching, but the neural basis of the spatial and temporal aspects of manual interception are largely unknown. Here, we used repetitive transcranial magnetic stimulation (rTMS) to investigate the role of the human middle temporal visual motion area (MT+/V5) and superior parieto-occipital cortex (SPOC) in the spatial and temporal control of manual interception. Participants were required to reach-to-intercept a downward moving visual target that followed an unpredictably curved trajectory, presented on a screen in the vertical plane. We found that rTMS to MT+/V5 influenced interceptive timing and positioning, whereas rTMS to SPOC only tended to increase the spatial variance in reach end points for selected target trajectories. These findings are consistent with theories arguing that distinct neural mechanisms contribute to spatial, temporal, and spatiotemporal control of manual interception

Homing and in situ differentiation of resident pulmonary lymphocytes

At birth, T lymphocytes which colonize the lung are mainly of the γδ subset, while αβ T cells predominate in the spleen. Thus, the lung is a preferred site for the homing of γδ T cells in the perinatal period. However, after birth, the pattern of Vγ gene usage among resident pulmonary lymphocytes (RPL) changes with age, from a predominance of Vγ6 at birth to a predominance of Vγ4 in older mice. The generation of the Vγ6 fraction appears to be thymus dependent, since in athymic nude mice, the Vγ6 population present at birth is replaced by Vγ4 T cells. In the postnatal period, both RAG-1 and RAG-2 genes are expressed at high levels in the RPL population. TCR bearing cells are among those that express RAG genes, indicating that maturation of T cells takes place in this organ. In addition, transfer experiments reveal that lymphoid precursors are present in the lung. The stage of differentiation of these precursors will be characterized in future studies. The data presented here indicate that pulmonary T lymphocytes are derived from both migrants of thymic origin and from precursors which have undergone differentiation and selection in the lung. The population that is generated in situ and that has not been selected in the thymus may include cells that are typical for the pulmonary environmen

Spatial biases in motion extrapolation for manual interception

The exact mechanisms by which humans control the manual interception of moving targets are currently unknown. Here, we explored the behaviours associated with the spatial control for manual interception. The examined task required controlling a cursor to intercept moving targets on a touch screen. We explored the effects of target motion direction, curvature and occlusion on manual interception. We observed occlusion-dependent spatial errors and arrival times for curved and diagonal trajectories (larger errors and earlier arrival of the finger at its final position with longer occlusion. These effects were particularly apparent for targets moving away from screen centre at interception due to curve. In a follow-up experiment we showed that the outward curve effects on spatial errors were absent because the associated trajectories appears to move towards positions that participants could expect the target to never reach. Our analyses also revealed occlusion-dependent spatial errors for diagonal trajectories, which is well-known angle-of-approach effect. Follow-up experiments demonstrated that this effect was not due to the central initial cursor position acting as a visual reference point or the initial ocular pursuit. Most importantly, the angle-of-approach effect persisted in a judgment task. We thus conclude that this effect does not stem from online information-based modulations of movement speed, but from target information used to control aiming (i.e., movement direction). Moreover, processing for diagonal target motion appears to be biased towards straight downwards

TREM1/3 deficiency impairs tissue repair after acute kidney injury and mitochondrial metabolic flexibility in tubular epithelial cells

Long-term sequelae of acute kidney injury (AKI) are associated with incomplete recovery of renal function and the development of chronic kidney disease (CKD), which can be mediated by aberrant innate immune activation, mitochondrial pathology, and accumulation of senescent tubular epithelial cells (TECs). Herein, we show that the innate immune receptor Triggering receptor expressed on myeloid cells-1 (TREM-1) links mitochondrial metabolism to tubular epithelial senescence. TREM-1 is expressed by inflammatory and epithelial cells, both players in renal repair after ischemia/reperfusion (IR)-induced AKI. Hence, we subjected WT and TREM1/3 KO mice to different models of renal IR. TREM1/3 KO mice displayed no major differences during the acute phase of injury, but increased mortality was observed in the recovery phase. This detrimental effect was associated with maladaptive repair, characterized by persistent tubular damage, inflammation, fibrosis, and TEC senescence. In vitro, we observed an altered mitochondrial homeostasis and cellular metabolism in TREM1/3 KO primary TECs. This was associated with G2/M arrest and increased ROS accumulation. Further exposure of cells to ROS-generating triggers drove the cells into a stress-induced senescent state, resulting in decreased wound healing capacity. Treatment with a mitochondria anti-oxidant partly prevented the senescent phenotype, suggesting a role for mitochondria herein. In summary, we have unraveled a novel (metabolic) mechanism by which TREM1/3 deficiency drives senescence in TECs. This involves redox imbalance, mitochondrial dysfunction and a decline in cellular metabolic activities. These finding suggest a novel role for TREM-1 in maintaining tubular homeostasis through regulation of mitochondrial metabolic flexibility

Kennisagenda Geo-informatie: GISsen met beleid

LNV wil méér geo-informatie inzetten bij de ontwikkeling en uitvoering van beleid en beleidsnota’s ruimer voorzien van kaartmateriaal. Dit betekent dat geo-informatie vaker moet worden benut om lokale knelpunten, mogelijkheden en de gevolgen van alternatieve oplossingen inzichtelijk te maken. Om dit te bereiken moet de beschikbaarheid van adequate data en gebruikersvriendelijke en nieuwe GIS-technieken aanmerkelijk verbeteren