Integrated survey of boat-based recreational fishing in inner Shark Bay 2018/19

Inner Shark Bay is a popular recreational fishing destination. As recreational fishing occurs in a World Heritage Area and Marine Park, there is a need for ongoing reliable information on catches. Many boat-based recreational fishers in inner Shark Bay target Pink Snapper (Chrysophrys auratus)

Review of recreational fishing surveys in Western Australia

Recreational fishing has been a popular activity in Western Australia for many years, with an estimated 25% of the population participating in this activity in 2017/18. Recreational fishing surveys have been implemented by the Department of Primary Industries and Regional Development (formally Department of Fisheries) since 1976

Framework for Integration of Data from Remotely Operated Cameras into Recreational Fishery Assessments in Western Australia

Remotely operated cameras can be used for cost-efficient monitoring of recreational fishing activities. This report provides an overview of the current usage of cameras in recreational fishery assessments by the Department of Primary Industries and Regional Development. Since 2006, 32 remotely operated cameras have been installed at 26 locations throughout Western Australia and currently 28 cameras are in use

Architecture of the yeast elongator complex

The highly conserved eukaryotic Elongator complex performs specific chemical modifications on wobble base uridines of tRNAs, which are essential for proteome stability and homeostasis. The complex is formed by six individual subunits (Elp1-6) that are all equally important for its tRNA modification activity. However, its overall architecture and the detailed reaction mechanism remain elusive. Here, we report the structures of the fully assembled yeast Elongator and the Elp123 sub-complex solved by an integrative structure determination approach showing that two copies of the Elp1, Elp2, and Elp3 subunits form a two-lobed scaffold, which binds Elp456 asymmetrically. Our topological models are consistent with previous studies on individual subunits and further validated by complementary biochemical analyses. Our study provides a structural framework on how the tRNA modification activity is carried out by Elongator

Live cell dynamics of production, explosive release and killing activity of phage tail-like weapons for Pseudomonas kin exclusion.

Interference competition among bacteria requires a highly specialized, narrow-spectrum weaponry when targeting closely-related competitors while sparing individuals from the same clonal population. Here we investigated mechanisms by which environmentally important Pseudomonas bacteria with plant-beneficial activity perform kin interference competition. We show that killing between phylogenetically closely-related strains involves contractile phage tail-like devices called R-tailocins that puncture target cell membranes. Using live-cell imaging, we evidence that R-tailocins are produced at the cell center, transported to the cell poles and ejected by explosive cell lysis. This enables their dispersal over several tens of micrometers to reach targeted cells. We visualize R-tailocin-mediated competition dynamics between closely-related Pseudomonas strains at the single-cell level, both in non-induced condition and upon artificial induction. We document the fatal impact of cellular self-sacrifice coupled to deployment of phage tail-like weaponry in the microenvironment of kin bacterial competitors, emphasizing the necessity for microscale assessment of microbial competitions

Travelers With Cutaneous Leishmaniasis Cured Without Systemic Therapy

Guidelines recommend wound care and/or local therapy as first-line treatment for cutaneous leishmaniasis. An analysis of a referral treatment program in 135 travelers showed that this approach was feasible in 62% of patients, with positive outcome in 83% of evaluable patient

MpUL-multi: Software for Calculation of Amyloid Fibril Mass per Unit Length from TB-TEM Images.

Structure determination for amyloid fibrils presents many challenges due to the high variability exhibited by fibrils and heterogeneous morphologies present, even in single samples. Mass per unit length (MPL) estimates can be used to differentiate amyloid fibril morphologies and provide orthogonal evidence for helical symmetry parameters determined by other methods. In addition, MPL data can provide insight on the arrangement of subunits in a fibril, especially for more complex fibrils assembled with multiple parallel copies of the asymmetric unit or multiple twisted protofilaments. By detecting only scattered electrons, which serve as a relative measure of total scattering, and therefore protein mass, dark field imaging gives an approximation of the total mass of protein present in any given length of fibril. When compared with a standard of known MPL, such as Tobacco Mosaic Virus (TMV), MPL of the fibrils in question can be determined. The program suite MpUL-multi was written for rapid semi-automated processing of TB-TEM dark field data acquired using this method. A graphical user interface allows for simple designation of fibrils and standards. A second program averages intensities from multiple TMV molecules for accurate standard determination, makes multiple measurements along a given fibril, and calculates the MPL

Recreational fishing for Western Rock Lobster: estimates of participation, effort and catch from 2018/19–2020/21. Fisheries Research Report 313

The Western Rock Lobster (WRL) (Panulirus cygnus) is one of the four Rock Lobster (RL) species found in Western Australia. It was the first fishery in the world to obtain Marine Stewardship Council Certification and the first in Western Australia to be managed under a resource allocation process. Catches from the commercial and recreational sectors are required to determine and monitor Total Allowable Catch (TAC), Total Allowable Recreational Catch (TARC) and proportion of Allowable Harvest Level (AHL) achieved. The TARC is set at 5% of the AHL and evaluated in the harvest strategy based on a 5-year average. Since 2018/19, data for the recreational sector have been collected using phone-recall surveys to provide estimates of participation, fishing effort and retained catch (by numbers) from recreational fishers who hold a RL licence. Average weight, obtained from boat ramp surveys, are used to convert estimates of retained catch from licensed fishers by number to weight. Tour Operator Returns (Charter Logbooks) provide a census of the annual participation, effort and retained catch (by numbers) from charter fishers. Random length samples of WRL are provided by tour operators and converted to weight using a length-weight equation which are then used to covert the retained catch from numbers to weight. Participation in the RL recreational fishery (all species) by licensed fishers (RL licence holders aged five years and older) in 2020/21 (1 February 2020–31 January 2021) was 66.8% or 37,469 fishers (95% CI 35,350–39,587). These values were steady from 2018/19–2020/21 (i.e., the 95% CI overlapped between years). The total fishing effort for RL fishing (all species) in 2020/21 was 522,485 days fished (95%CI 491,063–553,907); of which 76.9% or 401,600 (363,338–439,862) was by potting and 23.1% or 120,885 (93,904–147,866) by diving. These values were steady from 2018/19–2020/21. The majority of fishing effort occurred in the Metro-West Coast region (63.5–68.4%). The retained recreational catch of WRL by licensed fishers in 2020/21, based on an overall (i.e., combined across potting and diving) average weight of 587.4 g, was 526 t (95% CI 460–592); of which 79.8% or 420 t (362–478) was harvested by potting and 20.2% or 106 t (68–145) by diving. Total catch (in tonnes) was steady from 2018/19–2020/21. Retained recreational catch of WRL from charter fishing in 2020/21 was 16 t (based on an overall average weight of 494.9 g), an increase from 9 t in 2018/19 (based on an overall average weight of 569.0 g). The majority of the charter catch was taken by potting (88.9–93.7%). The 5-year average recreational catch (for licensed and charter fishing combined) was 427 t in 2018/19, which represents an AHL of 4.6%. The AHL increased to 4.9% in 2019/20 and 2020/21 with a 5-year average recreational catch of 468 t and 498 t, respectively

Recreational fishing for Western Rock Lobster: estimates of participation, effort and catch from 2018/19–2020/21. Fisheries Research Report 313

The Western Rock Lobster (WRL) (Panulirus cygnus) is one of the four Rock Lobster (RL) species found in Western Australia. It was the first fishery in the world to obtain Marine Stewardship Council Certification and the first in Western Australia to be managed under a resource allocation process. Catches from the commercial and recreational sectors are required to determine and monitor Total Allowable Catch (TAC), Total Allowable Recreational Catch (TARC) and proportion of Allowable Harvest Level (AHL) achieved. The TARC is set at 5% of the AHL and evaluated in the harvest strategy based on a 5-year average. Since 2018/19, data for the recreational sector have been collected using phone-recall surveys to provide estimates of participation, fishing effort and retained catch (by numbers) from recreational fishers who hold a RL licence. Average weight, obtained from boat ramp surveys, are used to convert estimates of retained catch from licensed fishers by number to weight. Tour Operator Returns (Charter Logbooks) provide a census of the annual participation, effort and retained catch (by numbers) from charter fishers. Random length samples of WRL are provided by tour operators and converted to weight using a length-weight equation which are then used to covert the retained catch from numbers to weight. Participation in the RL recreational fishery (all species) by licensed fishers (RL licence holders aged five years and older) in 2020/21 (1 February 2020–31 January 2021) was 66.8% or 37,469 fishers (95% CI 35,350–39,587). These values were steady from 2018/19–2020/21 (i.e., the 95% CI overlapped between years). The total fishing effort for RL fishing (all species) in 2020/21 was 522,485 days fished (95%CI 491,063–553,907); of which 76.9% or 401,600 (363,338–439,862) was by potting and 23.1% or 120,885 (93,904–147,866) by diving. These values were steady from 2018/19–2020/21. The majority of fishing effort occurred in the Metro-West Coast region (63.5–68.4%). The retained recreational catch of WRL by licensed fishers in 2020/21, based on an overall (i.e., combined across potting and diving) average weight of 587.4 g, was 526 t (95% CI 460–592); of which 79.8% or 420 t (362–478) was harvested by potting and 20.2% or 106 t (68–145) by diving. Total catch (in tonnes) was steady from 2018/19–2020/21. Retained recreational catch of WRL from charter fishing in 2020/21 was 16 t (based on an overall average weight of 494.9 g), an increase from 9 t in 2018/19 (based on an overall average weight of 569.0 g). The majority of the charter catch was taken by potting (88.9–93.7%). The 5-year average recreational catch (for licensed and charter fishing combined) was 427 t in 2018/19, which represents an AHL of 4.6%. The AHL increased to 4.9% in 2019/20 and 2020/21 with a 5-year average recreational catch of 468 t and 498 t, respectively