59 research outputs found
Canine Coronavirus Highly Pathogenic for Dogs
Canine coronavirus (CCoV) is usually responsible for mild, self-limiting infections restricted to the enteric tract. We report an outbreak of fatal disease in puppies caused by a pathogenic variant of CCoV that was isolated from organs with severe lesions
Molecular Epidemiology of Canine Parvovirus, Europe
Canine parvovirus (CPV), which causes hemorrhagic enteritis in dogs, has 3 antigenic variants: types 2a, 2b, and 2c. Molecular method assessment of the distribution of the CPV variants in Europe showed that the new variant CPV-2c is widespread in Europe and that the viruses are distributed in different countries
Norovirus in Captive Lion Cub (Panthera leo)
African lions (Panthera leo) are susceptible to viral diseases of domestic carnivores, including feline calicivirus infection. We report the identification of a novel enteric calicivirus, genetically related to human noroviruses of genogroup IV, in a lion cub that died of severe hemorrhagic enteritis
Detection and Molecular Characterization of a Canine Norovirus
We identified a novel calicivirus in a pup with enteritis. The isolate was related genetically (90.1% aa identity in the capsid protein) to a lion norovirus strain
Long‐term persistence of neutralizing SARS‐CoV‐2 antibodies in pets
We monitored the severe acute respiratory syndrome coronavirus 2 antibody response in seven dogs and two cats by using two multispecies ELISA tests, plaque reduction neutralisation test and virus neutralization. SARS-CoV-2 neutralizing antibodies in pets persisted up to 10 months since the first positive testing, thus replicating observations in COVID-19 human patients
Canine parvovirus epidemiology in Bulgaria
Canine parvovirus 2 (CPV-2) emerged in 1978 as one of the most pathogenic etiologic agents in dogs. Under the influence of evolution, the original CPV-2 was replaced, a few years later, by 2 variants, CPV-2a and CPV-2b. In 2000, a new variant, CPV-2c, was detected first in Italy and later in other countries. The current study was conducted to provide data about the CPV types circulating in Bulgaria. Forty-two fecal samples from dogs with clinical signs of parvovirosis, collected between June 2009 and February 2010, were tested for CPV using a rapid test based on detection of CPV antigens and a real-time polymerase chain reaction (PCR) for detection of viral DNA. Positive samples were characterized by means of minor groove binder probe PCR assays. Forty samples were positive, of which 30 were identified as CPV-2a, 9 as CPV-2b, and 1 as CPV-2c. The results from this molecular investigation of CPV show the prevalence of type 2a and occurrence of type 2c for the first time in Bulgaria
Canine parvovirus type 2c infection in a kitten associated with intracranial abscess and convulsions
A case of canine parvovirus type 2c (CPV-2c) infection in a 3-month-old feral kitten with a cerebral abscess and neurological disease is reported. The cat displayed ataxia and convulsions together with signs of gastroenteritis and profound alteration of the total and differential white blood cell counts. A parvovirus strain was detected by a TaqMan assay in the blood and faeces of the affected kitten, which was characterised as CPV by means of molecular assays but did not react with any of the CPV type-specific probes. By sequence and phylogenetic analyses of the VP2-protein gene, the CPV-2c strain displayed a non-coding mutation in the probe-binding region. Although the role of CPV-2c in this particular case is unclear, it is possible that it predisposed the kitten to the clinical signs seen. Continuous surveillance is needed to monitor future spreading of this CPV-2c mutant, and any associated clinical signs, in the dog and cat populatio
Occurrence of canine parvovirus-2 variants in Abruzzo region [Indagine sulla diffusione delle nuove varianti del parvovirus canino tipo 2 in Abruzzo]
Aim of the study - The diagnostic investigation results for canine parvovirus type 2 (CPV-2) infection in symptomatic dogs are reported, in order to evaluate the presence and the distribution of the viral variants in the canine population of Abruzzo region.
Materials and methods - One hundred and four faecal samples were analyzed by conventional and real-time PCR with minor groove binder (MGB) probes.
Results - CPV-2 DNA was detected in 55 (52,8%) samples and 31 (56,3%) CPV-2a, 1 (1,8%) CPV-2b and 23 (41,8%) CPV-2c strains were identified by real-time PCR with MGB probes.The distribution of CPV-2 over the sampling years appears different in relation to each variant.
Conclusions - CPV-2 is an important causative agent of gastroenteritis in dogs of the Abruzzo region and the CPV-2a was the most prevalent variant. However, it appears that the CPV-2c occurrence is growing, while CPV-2b is poorly widespread. In conclusion, the monitoring of the parvovirus infection in dogs population is useful to verify the evolution of the geographic distribution of the CPV-2 variants and to evaluate the efficacy of the prophylactic measures performed to control the disease
Evaluation of the lactogenic immunity to canine parvovirus in pups. New Microbiologica
Maternally-derived antibodies (MDA) transferred to pups through colostrum and milk are known as lactogenic immunity. In this report, we describe the kinetics of transfer of lactogenic immunity to canine parvovirus type 2 (CPV-2) from two bitches (A and B) to their offspring. At day 7 before parturition, bitches A and B had high serum antibody titers, which decreased rapidly within a few hours after parturition, in concomitance with the appearance of high HI titers in colostrum. Subsequently, the serum antibodies of the two dogs increased again, reaching approximately the initial titers. CPV-specific antibodies were observed in milk, with decreasing values, throughout the lactation period. The kinetics of MDA observed in the pups was consistent with the patterns of absorption and decline previously described
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