Frozen section evaluation of lymph nodes in Oral squamous cell carcinoma: a retrospective study

The principal indication of performing a frozen section is to obtain a definite immediate diagnosis, on the basis of which a surgical decision will be made. Immediate diagnosis allows the surgeon to proceed with further therapy without any delay. A definite immediate diagnosis is said to be dependent on the reliability of frozen sections. Many authors have carried out frozen section study on soft tissue tumors and lymph node evaluation in different parts of the body with varying percentages of reliability. However, it appears that lymph node evaluations specifically in oral squamous cell carcinoma cases were not performed, or limited. Hence the present study was undertaken to determine the reliability of frozen section diagnosis in the lymph nodes of oral squamous cell carcinomas. This study was performed so that the surgeons would have an intraoperative assessment of the extent of local metastasis and to determine the adequacy of resection before the wound is closed. The objectives of the present study are to assess microscopically the frozen section of the palpable and non palpable lymph nodes of neck for metastasis in patients with squamous cell carcinoma of oral cavity. To compare the micros- copic features of paraffin embedded lymph node sections with those of frozen sections. To assess the reliability of frozen section evaluation of cervical lymph nodes in oral squamous cell carcinoma. The present research work gives 100% accuracy of frozen sections with due care by additional staining to frozen sections by phloxine methylene blue stain, however cannot be in any way superior to the magnetic resonance imaging, computed tomography, immunoscintigraphy and lymphangioscintigraphy

Fibroblastic Variant of Osteosarcoma: A Challenge in Diagnosis & Management

Osteosarcoma of the jaws is a relative rare malignant bone tumor. Like, its counterpart in the long bones, osteosarcoma affecting the head and neck region shows distinct yet diverse clinical, histologic and prognostic characteristics. Its diagnosis is a challenge to histopathologists and is especially important in early stages to improve its prognosis. In the initial phase, it may present as nondescript swelling with an indolent growth rate, only to become overtly aggressive and malignant towards the later phase of the disease. This article reports on a case of an advanced osteosarcoma of the maxilla in a 42 year old woman who came for the evaluation of a swelling. The case was submitted for surgical intervention and was followed by one recurrence till the time of reporting. This case illustrates that immunohistochemical staining of Vimentin, S-100 and CD 68 markers are useful to confirm the histologic diagnosis of osteosarcoma, along with radiographic evaluation using CT scan and 3D imaging

Effect of herbicides on proteins in spring wheat

Three varieties of spring wheat, Selkirk, Manitou and Pembina, were grown in the field and greenhouse. 2,4-D, bromoxynil and picloram were applied at the 6" stage (4 leaf). The experiments were carried out in a randomized block design. 2,4-D and picloram showed a low concentration stimulation and high concentration inhibition on yield with Selkirk. Manitou and Pembina did not show definite trends with these two herbicides. Bromoxynil decreased the yield in all three varieties and the decrease was enhanced at higher concentrations. Bromoxynil was the only herbicide that increased the percent protein in all three varieties. An inverse relation between the percent protein and yield was seen in Selkirk, Manitou and Pembina treated with bromoxynil. 2,4-D and picloram also produced this relationship in Selkirk but not in Manitou or Pembina. Bromoxynil significantly reduced the yield of protein per plot, whereas 2,4-D and picloram did not. There was no quantitative relation between the protein extracted by 0.1 M Tris-glycine buffer (pH 8.3) or 0.05 N acetic acid (pH 3.5) and the percent protein in wheat. Selkirk was least affected by any of the herbicides, i.e. to the changes in the protein (albumin, globulin and gluten) as seen from the densitometric traces of gels. Manitou and Pembina showed changes as a result of the bromoxynil treatment.Land and Food Systems, Faculty ofGraduat

Immunohistochemical expression of phosphatase and tensin homolog in histologic gradings of oral squamous cell carcinoma

Context: Phosphatase and tensin homolog (PTEN) is a tumor suppressor gene located on chromosome 10q23. PTEN has its major function in the regulation of cell adhesion, cell cycle arrest, migration, apoptosis programming, and differentiation. This genomic region suffers loss of heterozygosity in many human cancers. Aims: The aim of this study was to compare the immunohistochemical expression of PTEN in normal oral mucosa and oral squamous cell carcinoma (OSCC) and to correlate the PTEN expression in gradings of OSCC. Materials and Methods: Thirty cases of paraffin tissue sections of previously diagnosed OSCC were taken. Of thirty cases, ten were well differentiated, ten were moderately differentiated, and ten were poorly differentiated. As a control, ten paraffin sections of oral normal mucosa tissue specimens were taken from patients undergoing extractions. The sections were stained for immunohistochemical expression of PTEN. The cells stained by PTEN antibody were counted, and an immunohistochemical score was obtained. Statistical Analysis Used: Statistical analysis was done using Mann–Whitney's test and Kruskal–Wallis test. Results: Statistical analysis revealed that there was a significant difference between normal mucosa and OSCC in immunohistochemistry staining. However, there was no significant difference in PTEN expression among gradings of OSCC. Conclusions: The study concluded that there was a decrease in PTEN expression in OSCC than normal mucosa. It also concluded that PTEN is a tumor suppressor gene which has a wide role in oral carcinogenesis

An unusual presentation of mixed tumor of salivary gland

Pleomorphic adenoma is one of the salivary gland tumors affecting both major and minor salivary glands. Parotid gland is the most commonly affected of the major group, and hard palate is the most common site of the minor salivary glands affected. Pleomorphic adenoma is a benign mixed tumor of the salivary glands that has elements of both epithelial and mesenchymal tissues. We report a case of pleomorphic adenoma of minor salivary glands in the soft palate. Incisional biopsy revealed features of pleomorphic adenoma