Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid

Sialic acids are located at the termini of mammalian cell-surface glycostructures, which participate in essential interaction processes including adhesion of pathogens prior to infection and immunogenicity. Here we present the synthesis and bioorthogonal metabolic incorporation of the sialic acid analogue N-(1-oxohex-5-ynyl)neuraminic acid (Neu5Hex) into the cell-surface glycocalyx of a human larynx carcinoma cell line (HEp-2) and its fluorescence labelling by click chemistry

Impact of DNI nowcasting on annual revenues of CSP plants for a time of delivery based feed in tariff

The impact of a combined nowcast for direct normal irradiation on revenues of two different concentrating solar power plant technologies under a time-of delivery-tariff is investigated. The nowcasts merge several satellite and NWP based nowcasts together with a smart persistence approach in order to generate a best-of nowcast for all now- and forecast time horizons up to 9 hours and in a 15 min temporal resolution. The applied tariff scheme is similar to that used in South Africa for some of the CSP plants erected in that country with considerably increased remuneration during the evening hours with maximum energy demand. The study investigates the impact both with respect to the annual energy yield and the economic annual revenues of CSP plants. The results for the combined nowcast are compared with results using ECMWF IFS based DNI forecasts which are commercially available for plant operators as well as with a persistence approach and the ideal forecast derived from ground-based observations