13 research outputs found
A note on divergent selection for total fleece weight in adult Angora rabbits: direct response to selection on total fleece weight at first and second harvest
[EN] In order to explore the genetic variability of wool production and other quantitative traits, an 8-cohort divergent selection experiment for total fleece weight (TFW) was carried out in French Angora rabbits. Studies were made on the wool production of a total of 669 female rabbits born between 1994 and 2001 and having produced wool from first to 12th harvests. The aim of the selection experiment was to obtain two divergent lines (low and high) on TFW. From preliminary analysis, the dataset was separated into three subsets according to the harvest number: one for each of the first two harvests and one for the third to the 12th harvests. In this paper, wool production data of the first and second harvests was analysed separately. Response to selection for total fleece weight at 3-12 harvest (TFW3-12) on this trait at first and second harvest was the aim of this paper. The second objective was to study the possibility of utilising values of the first or second harvest to estimate breeding values and as selection criteria for total fleece weight in the French Angora rabbit. Preliminary analysis of the data for non-genetic factors was done by the GLM procedure of SAS. Genetic parameters and breeding value estimates were carried out using a BLUP animal model using ASReml. A linear mixed model for a bivariate analysis of total fleece weight at first or second harvest and TFW3-12 was used. Heritability estimates of total fleece weight at first and second harvests were 0.36 and 0.38, respectively, and were similar to that observed at later harvests (0.35). The genetic correlation between TFW3-12 and fleece weight at first harvest was close to zero indicating that wool production at first harvest is a different trait from that of subsequent harvests. Genetic correlation estimates observed at second harvest were high (0.76) and response to selection at second harvest was similar to that observed for TFW3-12. These observations confirm that total fleece weight at first harvest is a different trait from TFW3-12. In French Angora rabbits, the high genetic correlation between TFW3-12 and total fleece weight at second harvest suggests the possibility of selection at this time for TFW3-12.The authors thank Gérard Auvinet, Jean Claude Musseau and Patricia Bayle of the Institut National de la
Recherche Agronomique (INRA), Génétique Expérimentale en Productions Animales, Le Magneraud Poitou-Charentes Research
Centre for the collection of data and the supply and care of Angora rabbits.Rafat, S.; Thébault, R.; Bonnet, M.; Deretz, S.; Pena-Arnaud, B.; De Rochambeau, H.; Allain, D. (2009). A note on divergent selection for total fleece weight in adult Angora rabbits: direct response to selection on total fleece weight at first and second harvest. World Rabbit Science. 17(1):39-44. doi:10.4995/wrs.2009.669394417
LIPH Expression in Skin and Hair Follicles of Normal Coat and Rex Rabbits
Natural mutations in the LIPH gene were shown to be responsible for hair growth defects in humans and for the rex short hair phenotype in rabbits. In this species, we identified a single nucleotide deletion in LIPH (1362delA) introducing a stop codon in the C-terminal region of the protein. We investigated the expression of LIPH between normal coat and rex rabbits during critical fetal stages of hair follicle genesis, in adults and during hair follicle cycles. Transcripts were three times less expressed in both fetal and adult stages of the rex rabbits than in normal rabbits. In addition, the hair growth cycle phases affected the regulation of the transcription level in the normal and mutant phenotypes differently. LIPH mRNA and protein levels were higher in the outer root sheath (ORS) than in the inner root sheath (IRS), with a very weak signal in the IRS of rex rabbits. In vitro transfection shows that the mutant protein has a reduced lipase activity compared to the wild type form. Our results contribute to the characterization of the LIPH mode of action and confirm the crucial role of LIPH in hair production
A Deletion in Exon 9 of the LIPH Gene Is Responsible for the Rex Hair Coat Phenotype in Rabbits (Oryctolagus cuniculus)
The fur of common rabbits is constituted of 3 types of hair differing in length and diameter while that of rex animals is essentially made up of amazingly soft down-hair. Rex short hair coat phenotypes in rabbits were shown to be controlled by three distinct loci. We focused on the “r1” mutation which segregates at a simple autosomal-recessive locus in our rabbit strains. A positional candidate gene approach was used to identify the rex gene and the corresponding mutation. The gene was primo-localized within a 40 cM region on rabbit chromosome 14 by genome scanning families of 187 rabbits in an experimental mating scheme. Then, fine mapping refined the region to 0.5 cM (Z = 78) by genotyping an additional 359 offspring for 94 microsatellites present or newly generated within the first defined interval. Comparative mapping pointed out a candidate gene in this 700 kb region, namely LIPH (Lipase Member H). In humans, several mutations in this major gene cause alopecia, hair loss phenotypes. The rabbit gene structure was established and a deletion of a single nucleotide was found in LIPH exon 9 of rex rabbits (1362delA). This mutation results in a frameshift and introduces a premature stop codon potentially shortening the protein by 19 amino acids. The association between this deletion and the rex phenotype was complete, as determined by its presence in our rabbit families and among a panel of 60 rex and its absence in all 60 non-rex rabbits. This strongly suggests that this deletion, in a homozygous state, is responsible for the rex phenotype in rabbits
A Deletion in Exon 9 of the LIPH Gene Is Responsible for the Rex Hair Coat Phenotype in Rabbits (Oryctolagus cuniculus)
The fur of common rabbits is constituted of 3 types of hair differing in length and diameter while that of rex animals is essentially made up of amazingly soft down-hair. Rex short hair coat phenotypes in rabbits were shown to be controlled by three distinct loci. We focused on the “r1” mutation which segregates at a simple autosomal-recessive locus in our rabbit strains. A positional candidate gene approach was used to identify the rex gene and the corresponding mutation. The gene was primo-localized within a 40 cM region on rabbit chromosome 14 by genome scanning families of 187 rabbits in an experimental mating scheme. Then, fine mapping refined the region to 0.5 cM (Z = 78) by genotyping an additional 359 offspring for 94 microsatellites present or newly generated within the first defined interval. Comparative mapping pointed out a candidate gene in this 700 kb region, namely LIPH (Lipase Member H). In humans, several mutations in this major gene cause alopecia, hair loss phenotypes. The rabbit gene structure was established and a deletion of a single nucleotide was found in LIPH exon 9 of rex rabbits (1362delA). This mutation results in a frameshift and introduces a premature stop codon potentially shortening the protein by 19 amino acids. The association between this deletion and the rex phenotype was complete, as determined by its presence in our rabbit families and among a panel of 60 rex and its absence in all 60 non-rex rabbits. This strongly suggests that this deletion, in a homozygous state, is responsible for the rex phenotype in rabbits
Investigation of the Agouti gene for the identification of useful markers for coat colour association studies in domestic rabbits
Investigation of the Agouti gene for the identification of useful markers for coat colour association studies in domestic rabbits
A composite six bp in-frame deletion in the melanocortin 1 receptor (MC1R) gene is associated with the Japanese brindling coat colour in rabbits (Oryctolagus cuniculus)
In the domestic rabbit (Oryctolagus cuniculus), classical genetic studies have identified five alleles at the Extension locus: E D (dominant black), E S (steel, weaker version of E D ), E (wild type, normal extension of black), e J (Japanese brindling, mosaic distribution of black and yellow) and e (non-extension of black, yellow/red with white belly). Sequencing almost the complete coding sequence (CDS) of the rabbit MC1R gene, we recently identified two in-frame deletions associated with dominant black (c.280_285del6; alleles E D or E S ) and recessive red (c.304_333del30; allele e) coat colours. It remained to characterize the e J allele whose phenotypic effect is similar to the Orange and Sex-linked yellow loci of cat and Syrian hamster