41 research outputs found

    Speed breeding for multiple quantitative traits in durum wheat

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    Plant breeding requires numerous generations to be cycled and evaluated before an improved cultivar is released. This lengthy process is required to introduce and test multiple traits of interest. However, a technology for rapid generation advance named 'speed breeding' was successfully deployed in bread wheat (Triticum aestivum L.) to achieve six generations per year while imposing phenotypic selection for foliar disease resistance and grain dormancy. Here, for the first time the deployment of this methodology is presented in durum wheat (Triticum durum Desf.) by integrating selection for key traits, including above and below ground traits on the same set of plants. This involved phenotyping for seminal root angle (RA), seminal root number (RN), tolerance to crown rot (CR), resistance to leaf rust (LR) and plant height (PH). In durum wheat, these traits are desirable in environments where yield is limited by in-season rainfall with the occurrence of CR and epidemics of LR. To evaluate this multi-trait screening approach, we applied selection to a large segregating F2 population (n = 1000) derived from a bi-parental cross (Outrob4/Caparoi). A weighted selection index (SI) was developed and applied. The gain for each trait was determined by evaluating F3 progeny derived from 100 'selected' and 100 'unselected' F2 individuals.Transgressive segregation was observed for all assayed traits in the Outrob4/Caparoi F2 population. Application of the SI successfully shifted the population mean for four traits, as determined by a significant mean difference between 'selected' and 'unselected' F3 families for CR tolerance, LR resistance, RA and RN. No significant shift for PH was observed.The novel multi-trait phenotyping method presents a useful tool for rapid selection of early filial generations or for the characterization of fixed lines out-of-season. Further, it offers efficient use of resources by assaying multiple traits on the same set of plants. Results suggest that when performed in parallel with speed breeding in early generations, selection will enrich recombinant inbred lines with desirable alleles and will reduce the length and number of years required to combine these traits in elite breeding populations and therefore cultivars.Samir Alahmad, Eric Dinglasan, Kung Ming Leung, Adnan Riaz, Nora Derbal, Kai P. Voss-Fels, Jason A. Able, Filippo M. Bassi, Jack Christopher and Lee T. Hicke

    Towards a greater dialogue on disability between Muslims and Christians

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    Attitudes to disability and disabled people by Muslims – focusing on attitudes in the Middle East and North Africa - and Christians – focusing on the West (here taken to mean Europe, North America and Australasia) - were examined through a grounded theory literature search, with the study being divided into three phases of reading and analysis. The aims of study were to develop a dialogue on disability between the two cultures, to inform an understanding of the attitudes to disability in the two cultures, and to inform cultural practice in promoting support and equality in both cultures. The study finds that Islam and Christianity have much in common and are a force for good in promoting and developing disability equality in both Muslim and Christian cultures

    Artificial neural network (ANN) approach for modelling of pile settlement of open-ended steel piles subjected to compression load

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    This study was devoted to examine pile bearing capacity and to provide a reliable model to simulate pile load-settlement behaviour using a new artificial neural network (ANN) method. To achieve the planned aim, experimental pile load test were carried out on model open-ended steel piles, with pile aspect ratios of 12, 17, and 25. An optimised second-order Levenberg-Marquardt (LM) training algorithm has been used in this process. The piles were driven in three sand densities; dense, medium, and loose. A statistical analysis test was conducted to explore the relative importance and the statistical contribution (Beta and Sig) values of the independent variables on the model output. Pile effective length, pile flexural rigidity, applied load, sand-pile friction angle and pile aspect ratio have been identified to be the most effective parameters on model output. To demonstrate the effectiveness of the proposed algorithm, a graphical comparison was performed between the implemented algorithm and the most conventional pile capacity design approaches. The proficiency metric indicators demonstrated an outstanding agreement between the measured and predicted pile-load settlement, thus yielding a correlation coefficient (R) and root mean square error (RMSE) of 0.99, 0.043 respectively, with a relatively insignificant mean square error level (MSE) of 0.0019. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group

    Otolithometry and scalimetry—two valid methods to describe the growth of peacock wrasse, Symphodus tinca (Actinopterygii: Perciformes: Labridae) from eastern Algeria

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    Background. The peacock wrasse, Symphodus tinca (Linnaeus, 1758), is a common Mediterranean fish and the most abundant labrid species on the eastern coast of Algeria. Its age and growth, however, have been poorly studied. Such data are essential for proper fisheries management. The aim of this study is to provide information on the age and growth of this wrasse species from the eastern Algeria based on two different methods of fish age determination. Materials and methods. Age and growth of Symphodus tinca were determined by examining otoliths and scales of the fish sampled between January and December 2011 on the eastern coast of Algeria. Otoliths and scales from 515 and 469 specimens, respectively, were used to determine the age using commonly accepted procedures. Results. The age was successfully determined in 513 (99.61%) otoliths (277 females, 209 males) and in 428 (91.25%) scales (232 females, 173 males), representing age classes between 0+ and 5+ years (1+–3+ years for females and 1+–5+ years for males). The validity of the otolith and scale readings for estimating age and growth was supported by the back-calculation method. The success rate in reading otoliths and scales of Symphodus tinca was very high. The growth pattern is well described by the von Bertalanffy growth equation: from otoliths, L∞ = 26.46 cm, k = 0.79, t0 = –0.12 for females and L∞ = 32.32 cm, k = 0.54, t0 = –0.22 for males; from scales, L∞ = 26.61 cm, k = 0.61, t0 = –0.45 for females and L∞ = 32.50 cm, k = 0.48, t0 = –0.31 for males. Otolithometry and scalimetry gave very close results. The total lengths (TL) ranged from 4.9 to 31.3 cm, and eviscerated weight (We) from 1.38 to 400.65 g. The length–weight relation showed an isometric growth for females (b = 3.01), and negative allometric growth for males (b = 2.86). Conclusion. The age was estimated by otolith and scale reading for males and females where the two methods yielded the similar results. Although the otolith readings presented a better fit (99.61%), scales could also be used with fairly good results (91.25%). In the future, research on the growth of S. tinca either of these two methods can be used to save time. The scale method, however, is certainly the easiest

    Bile Duct Injury During Cholecystectomy: Necessity to Learn How to Do and Interpret Intraoperative Cholangiography.

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    Biliary duct injury (BDI) is a serious complication during cholecystectomy. Perioperative cholangiography (POC) has recently been generating interest in order to prevent BDI. However, the current literature (including randomized controlled trials) cannot conclude whether POC is protective or not against the risk of BDI. The aim of our study was to investigate whether POC could demonstrate earlier BDI and which criteria are required to make that diagnosis. We performed a retrospective study between 2005 and 2018 in our French tertiary referral center, which included all patients who had presented following BDI during cholecystectomy. Twenty-two patients were included. Nine patients had POC, whereas 13 did not. When executed, POC was interpreted as normal for three patients and abnormal for six. In this latter group, only two cases had a BDI diagnosed intraoperatively. In other cases, the interpretation was not adequate. BDIs are rare but may reduce patients' quality of life. Our study highlights the surgeon's responsibility to learn how to perform and interpret POC in order to diagnose and manage BDIs and potentially avoid catastrophic consequences
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