Effects of Low pH on Lactate Dehydrogenase Kinetics of Diving and Nondiving Reptiles

The properties of lactate dehydrogenase were examined in two snake species, Nerodia rhombifera and Elaphe obsoleta, and a turtle species, Pseudemys scripta. Our purpose was to compare the LDH activity of reptiles with limited anaerobic capabilities with that of the well established diver Pseudemys. The Michaelis-Menten kinetics of LDH and its susceptibility to inhibition by elevated pyruvate concentrations were investigated in the brain and heart of the three species. All tissue incubations and enzyme activity determinations were done at a pH of 7.0 in order to stimulate a diving blood pH in the three species. In both tissues the LDH activity of the snakes was higher than that of Pseudemys at pyruvate concentrations ranging between .03 mM and .50 mM. The Km values of the snakes were lower than those of Pseudemys, suggesting a greater enzyme-substrate affinity in the snake tissues. The Vmax values were higher in the snake tissues indicating a faster conversion of substrate to product. Heart LDH activity was reduced to an equal extent by high pyruvate concentrations in each of the three species. Elaphe brain LDH was most susceptible to pyruvate inhibition, but Nerodia and Pseudemys brain LDH were inhibited to an equal extent. The results indicate that the kinetic behavior of brain and heart LDH of the three species is similar at a pH of 7.4 and a pH of 7.0. The results also suggest that the LDH of Pseudemys is no better adapted to withstand anaerobic conditions than that of Nerodia or Elaphe at a pH of 7.0

Hard x ray highlights of AR 5395

Active Region 5395 produced an exceptional series of hard x ray bursts notable for their frequency, intensity, and impulsivity. Over the two weeks from March 6 to 19, 447 hard x ray flares were observed by the Hard X Ray Burst Spectrometer on Solar Maximum Mission (HXRBS/SMM), a rate of approx. 35 per day which exceeded the previous high by more than 50 percent. During one 5 day stretch, more than 250 flares were detected, also a new high. The three largest GOES X-flares were observed by HXRBS and had hard x ray rates over 100,000 s(exp -1) compared with only ten flares above 100,000(exp -1) during the previous nine years of the mission. An ongoing effort for the HXRBS group has been the correlated analysis of hard x ray data with flare data at other wavelengths with the most recent emphasis on those measurements with spatial information. During a series of bursts from AR 5395 at 1644 to 1648 UT on 12 March 1989, simultaneous observations were made by HXRBS and UVSP (Ultra Violet Spectrometer Polarimeter) on SMM, the two-element Owens Valley Radio Observatory (OVRO) interferometric array, and R. Canfield's H-alpha Echelle spectrograph at the National Solar Observatory at Sacramento Peak. The data show strong correlations in the hard x ray, microwave, and UV lightcurves. This event will be the subject of a combined analysis

Meteorological observations required for future weather modification programs

Meteorological observations required for computer models describing weather modification experiment

Adventitious shoot propagation and cultural inputs in nursery production of a primocane-fruiting blackberry selection

Studies were conducted from January to October 2005 to determine the effect of root-cutting length on adventitious shoot yield and the management practices necessary to produce nurseryquality primocane-fruiting blackberry plants. The first portion of the study measured the average number of shoots produced from 7.6 cm- and 15.2 cm-long root cuttings of APF-44 blackberry—a primocane-fruiting genotype from the University of Arkansas breeding program. Cuttings were forced in a shallow bin containing a soilless potting medium. The average number of shoots per root cutting from 7.6 cm- and 15.2 cm- long root cuttings averaged 1.6 and 2.7 shoots per root cutting, respectively. Rooting percentage for collected shoots was nearly 100% regardless of root-cutting length source. A qualitative comparison of shoots from the two roots lengths was similar. The latter part of the study included various treatments on the rooted shoots that might affect the productivity and quality of the final product intended for nursery sales in early fall. With the aim of producing a flowering/fruiting shrub by late September, three treatments were applied: pot dimension, fertilizer rate, and shoot tipping. Fertilizer rate had the greatest impact of all treatments with the higher rate producing larger and more attractive plants. Above-normal summer/fall temperatures may explain lack of fruiting on APF-44 blackberries, but the dimension and size of some plants provided a portion of the intended aesthetic

The magneto-optical Faraday effect in spin liquid candidates

We propose an experiment to use the magneto-optical Faraday effect to probe the dynamic Hall conductivity of spin liquid candidates. Theory predicts that an external magnetic field will generate an internal gauge field. If the source of conductivity is in spinons with a Fermi surface, a finite Faraday rotation angle is expected. We predict the angle to scale as the square of the frequency rather than display the standard cyclotron resonance pattern. Furthermore, the Faraday effect should be able to distinguish the ground state of the spin liquid, as we predict no rotation for massless Dirac spinons. We give a semiquantitative estimate for the magnitude of the effect and find that it should be experimentally feasible to detect in both $\kappa$-(ET)$_2$Cu$_2$(CN)$_3$ and, if the spinons form a Fermi surface, Herbertsmithite. We also comment on the magneto-optical Kerr effect and show that the imaginary part of the Kerr angle may be measurable.Comment: 5 pages, 1 figur

Geometric phases in 2D and 3D polarized fields: geometrical, dynamical, and topological aspects

Geometric phases are a universal concept that underpins numerous phenomena involving multi-component wave fields. These polarization-dependent phases are inherent in interference effects, spin-orbit interaction phenomena, and topological properties of vector wave fields. Geometric phases have been thoroughly studied in two-component fields, such as two-level quantum systems or paraxial optical waves. However, their description for fields with three or more components, such as generic nonparaxial optical fields routinely used in modern nano-optics, constitutes a nontrivial problem. Here we describe geometric, dynamical, and total phases calculated along a closed spatial contour in a multi-component complex field, with particular emphasis on 2D (paraxial) and 3D (nonparaxial) optical fields. We present several equivalent approaches: (i) an algebraic formalism, universal for any multi-component field; (ii) a dynamical approach using the Coriolis coupling between the spin angular momentum and reference-frame rotations; and (iii) a geometric representation, which unifies the Pancharatnam-Berry phase for the 2D polarization on the Poincar\'e sphere and the Majorana-sphere representation for the 3D polarized fields. Most importantly, we reveal close connections between geometric phases, angular-momentum properties of the field, and topological properties of polarization singularities in 2D and 3D fields, such as C-points and polarization M\"obius strips.Comment: 21 pages, 11 figures, to appear in Rep. Prog. Phy

Program for the feasibility of developing a high pressure acoustic levitator

This is the final report for the program for the feasibility of developing a high-pressure acoustic levitator (HPAL). It includes work performed during the period from February 15, 1987 to October 26, 1987. The program was conducted for NASA under contract number NAS3-25115. The HPAL would be used for containerless processing of materials in the 1-g Earth environment. Results show that the use of increased gas pressure produces higher sound pressure levels. The harmonics produced by the acoustic source are also reduced. This provides an improvement in the capabilities of acoustic levitation in 1-g. The reported processing capabilities are directly limited by the design of the Medium Pressure Acoustic Levitator used for this study. Data show that sufficient acoustic intensities can be obtained to levitate and process a specimen of density 5 g/cu cm at 1500 C. However, it is recommended that a working engineering model of the HPAL be developed. The model would be used to establish the maximum operating parameters of furnace temperature and sample density

Two Amino Acid Residues Contribute to a Cation-π Binding Interaction in the Binding Site of an Insect GABA Receptor

Cys-loop receptor binding sites characteristically possess an "aromatic box," where several aromatic amino acid residues surround the bound ligand. A cation-π interaction between one of these residues and the natural agonist is common, although the residue type and location are not conserved. Even in the closely related vertebrate GABA_A and GABA_C receptors, residues in distinct locations perform this role: in GABA_A receptors, a Tyr residue in loop A forms a cation-π interaction with GABA, while in GABA_C receptors it is a loop B residue. GABA-activated Cys-loop receptors also exist in invertebrates, where they have distinct pharmacologies and are the target of a range of pesticides. Here we examine the location of GABA in an insect binding site by incorporating a series of fluorinated Phe derivatives into the receptor binding pocket using unnatural amino acid mutagenesis, and evaluating the resulting receptors when expressed in Xenopus oocytes. A homology model suggests that two aromatic residues (in loops B and C) are positioned such that they could contribute to a cation-π interaction with the primary ammonium of GABA, and the data reveal a clear correlation between the GABA EC_(50) and the cation-π binding ability both at Phe206 (loop B) and Tyr254 (loop C), demonstrating for the first time the contribution of two aromatic residues to a cation-π interaction in a Cys-loop receptor