Dual function of GTPBP6 in biogenesis and recycling of human mitochondrial ribosomes

Translation and ribosome biogenesis in mitochondria require auxiliary factors that ensure rapid and accurate synthesis of mitochondrial proteins. Defects in translation are associated with oxidative phosphorylation deficiency and cause severe human diseases, but the exact roles of mitochondrial translation-associated factors are not known. Here we identify the functions of GTPBP6, a homolog of the bacterial ribosome-recycling factor HflX, in human mitochondria. Similarly to HflX, GTPBP6 facilitates the dissociation of ribosomes in vitro and in vivo. In contrast to HflX, GTPBP6 is also required for the assembly of mitochondrial ribosomes. GTPBP6 ablation leads to accumulation of late assembly intermediate(s) of the large ribosomal subunit containing ribosome biogenesis factors MTERF4, NSUN4, MALSU1 and the GTPases GTPBP5, GTPBP7 and GTPBP10. Our data show that GTPBP6 has a dual function acting in ribosome recycling and biogenesis. These findings contribute to our understanding of large ribosomal subunit assembly as well as ribosome recycling pathway in mitochondria

ЗАДАЧА СИНТЕЗА ИНДИВИДУАЛЬНЫХ ПЛАНОВ ОБУЧЕНИЯ В ПРОСТРАНСТВЕ ВИРТУАЛЬНЫХ УЧЕБНЫХ ГРУПП

The approach of educational planning and organization of educational process is offered to provide students the ability to select and adjust trajectories of learning. The mathematical description of curriculum and algorithm of synthesis individual curriculums are presented. The results of applying the algorithm are showed using an automated system.Рассмотрен подход учебного планирования и организации учебного процесса, предусматривающий возможность выбора и корректировки траектории обучения студентов. Представлено математическое описание учебных планов и алгоритм синтеза индивидуальных учебных планов. Приводятся результаты применения алгоритма с использованием автоматизированной системы

Detection of sexually transmitted infection and human papillomavirus in negative cytology by multiplex-PCR

<p>Abstract</p> <p>Background</p> <p>The aim of this study was to determine the prevalence of human papillomavirus (HPV) and 15 species that cause sexually transmitted infections (STIs) in negative cytology. In addition, we compared the diagnostic performance of multiplex polymerase chain reaction (PCR) with widely available techniques used to detect HPV.</p> <p>Methods</p> <p>We recruited 235 women of reproductive age who had negative cytology findings in a liquid-based cervical smear. STIs were identified by multiplex PCR, and HPV genotypes by multiplex PCR, hybrid capture 2, and DNA microaray; discordant results were analyzed by direct sequencing.</p> <p>Results</p> <p>Approximately 96.6% of patients with negative cytology results were positive for pathogens that cause STIs. The pathogens most frequently detected were <it>Gardnerella vaginalis, Ureaplasma urealyticum</it>. The incidence of HPV in negative cytology was 23.3%. Low-risk HPV infection was significantly correlated with <it>Chalmaydia trachomatis</it>, and high-risk HPV infection was significantly correlated with <it>Group β streptococcus</it>. The analytical sensitivities of the multiplex PCR and DNA microarray were higher than 80%, and the analytical specificity was nearly 100% for all tests.</p> <p>Conclusions</p> <p>Multiplex PCR yielded results that most of patients with negative cytology were positive for pathogens that cause STIs, and were more similar to that of DNA microarray, than that of hybrid capture 2 in terms of analytical sensitivity and prediction value of HPV infection.</p

Adaptive Antenna Against Multipath?

Improvements achieved in GNSS space and control segments as well as in receiver signal processing technologies leave multipath nowadays as the main challenge to the navigation society especially in the case of differential navigation. The use of an adaptive antenna in the navigation receiver can be evidently a prospective solution to the problem of multipath. It allows to discriminate incoming signals in spatial domain and to suppress multipath signals efficiently even when common temporal and frequency domain techniques fail. The adaptive antenna is also able to produce a large gain to the line-of-sight satellite signals and therefore to improve receiver performance in unfavourable signal conditions. This paper presents investigation results for the performance of a basic navigation receiver combined with the adaptive antenna, a 5-by-5 uniform rectangular antenna array. The performance is characterised in terms of the pseudorange error. Three multipath scenarios, namely severe multipath conditions, final approach and urban car, were used with signals of GPS and signals likely to be used in coming Galileo. The signals were tracked with the commonly used non-coherent DLL and Costas PLL. The results indicate that both the multipath induced bias and the standard deviation of the pseudorange error can be reduced up to one order of magnitude. While suppressing multipath echoes, the antenna provides more than 10 dB additional gain to the line-of-sight signal when compared to the standard antenna with a hemispherical diagram. In conclusion, the user equivalent range error (UERE) budget is compared for different types of GNSS receivers (single frequency, dual frequency and differential mode) taking into account the results for multipath error with the standard and adaptive antennas. It is shown that the use of adaptive antennas is the most advantageous for dual frequency receivers and receivers with differential mode

Improvement of UERE receiver performance due to multipath suppression with an adaptive antenna

In this paper we present results of modelling the User Equivalent Range Error (UERE) for a navigation receiver equipped with an adaptive 5×5 square antenna array. The antenna array was used as a spatial filter for short delayed multipath echoes in an urban environment. We simulated the receiver multipath pseudorange error with different navigation signals under signal conditions resulted from the use of the adaptive antenna. In order to assess the UERE for various types of navigation receivers, the obtained multipath error has been combined with standard values of other pseudorange error contributions

Exploiting the features of energy-dispersive synchrotron diffraction for advanced residual stress and texture analysis

Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.Responding to a growing interest from the materials science community for residual stress, texture, and microstructure analysis, strong efforts are made to enhance existing and develop novel methods that allow for fast in-situ studies at elevated temperature, measurements under external load, or residual strain, and stress scanning with high spatial resolution. In the paper, energy-dispersive diffraction using high-energy white synchrotron radiation is shown to provide some distinct advantages concerning residual stress and texture analysis, which mainly arise from the fact that the energy-dispersive diffraction mode allows for the measurement of complete diffraction patterns under fixed but arbitrary scattering angles, 2θ. A new two-detector set-up for simultaneous in- and out-of-plane diffraction analysis, which has been put into operation recently at the energy-dispersive materials science beamline EDDI at BESSY II, is introduced by using the examples of real-space residual stress and texture depth profiling on mechanically treated polycrystalline materials as well as of the in-situ study of (residual) stress evolution in a thin film at elevated temperature. It will be demonstrated that the individual measuring problems require the application of different geometrical slit configurations to define the pathways of the diffracted beams