Predictive context biases perceptual selection during binocular rivalry

Prediction may be a fundamental principle of sensory processing, such that the brain continuously generates predictions about forthcoming sensory information. However, little is known about how prediction contributes to the selection of a conscious percept from among competing alternatives. Here, we used binocular rivalry to investigate the effects of prediction on perceptual selection. In binocular rivalry, incompatible images presented to the two eyes result in a perceptual alternation between the images, even though the visual stimuli remain constant. If predictive signals influence the competition between neural representations of rivalrous images, this influence should generate a bias in perceptual selection that depends on predictive context. To manipulate predictive context, we developed a novel binocular rivalry paradigm in which orthogonal rivalrous test gratings were immediately preceded by rotating gratings presented identically to the two eyes. One of the rivalrous gratings had an orientation that was consistent with the preceding rotation direction (it was the expected next image in the series), and the other had an inconsistent orientation. We found that human observers were more likely to perceive the consistent grating, suggesting that predictive context biased selection in favor of the predicted percept. This prediction effect depended on only recent stimulus history, and it could be dissociated from another stimulus history effect related to orientation-specific adaptation. Since binocular rivalry between orthogonal gratings is thought to be resolved at an early stage of visual processing, these results suggest that predictive signals may exist at low levels of the visual processing hierarchy and that these signals can bias conscious perception. In the future, this paradigm could be used to test whether visual percepts are generated from the combination of prior information and incoming sensory information according to Bayesian principles

Structural and functional characterization of the aryl hydrocarbon receptor ligand binding domain by homology modeling and mutational analysis

The aryl hydrocarbon receptor (AhR) is a ligand-dependent transcription factor that is activated by a structurally diverse array of synthetic and natural chemicals, including toxic halogenated aromatic hydrocarbons such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Analysis of the occurring in the AhR ligand binding and activation processes requires structural information on the AhR Per-Arnt-Sim (PAS) B-containing ligand binding domain, for which no experimentally determined structure has been reported. With the availability of extensive structural information on homologous PAS-containing proteins, a reliable model of the mouse AhR PAS B domain was developed by comparative modeling techniques. The PAS domain structures of the functionally related hypoxia-inducible factor 2α (HIF-2α) and AhR nuclear translocator (ARNT) proteins, which exhibit the highest degree of sequence identity and similarity with AhR, were chosen to develop a two-template model. To confirm the features of the modeled domain, the effects of point mutations in selected residue positions on both TCDD binding to the AhR and TCDD-dependent transformation and DNA binding were analyzed. Mutagenesis and functional analysis results are consistent with the proposed model and confirm that the cavity modeled in the interior of the domain is indeed involved in ligand binding. Moreover, the physicochemical characteristics of some residues and of their mutants, along with the effects of mutagenesis on TCDD and DNA binding, also suggest some key features that are required for ligand binding and activation of mAhR at a molecular level, thus providing a framework for further studies. © 2007 American Chemical Society

Automated Carrier Landing of an Unmanned Combat Aerial Vehicle Using Dynamic Inversion

Dynamic Inversion (DI) is a powerful nonlinear control technique which has been applied to several modern flight control systems. This research utilized concepts of DI in order to develop a controller to land an Unmanned Combat Aerial Vehicle (UCAV) on an aircraft carrier. The Joint Unmanned Combat Air System (J-UCAS) Equivalent Model was used as the test aircraft. An inner-loop DI controller was developed to control the pitch, roll, and yaw rate dynamics of the aircraft, while an outer-loop DI controller was developed to provide flight path commands to the inner-loop. The controller design and simulation were conducted in the MATLAB/Simulink environment. Simulations were conducted for various starting positions near the carrier and for varying wind, wind turbulence, and sea state conditions. In the absence of wind and sea state turbulence, the controller performed well. After adding wind and sea state turbulence, the controller performance was degraded. Future work in this area should include a more robust disturbance rejection technique to compensate for wind turbulence effects and a method of carrier motion prediction to compensate for sea state effects

Does The Oil Pollution Act Of 1990 Rise To The Level Of A Taking? How The Court Missed The Boat In Maritrans, Inc. v. United States

A recent case in the Court of Federal Claims was the first test by owners of oil tankers and tank barges of section 4115 of the Oil Pollution Act of 1990 (OPA 90), otherwise known as the vessel construction requirement. The Court held that Maritrans, Inc., owners of thirty-seven tank barges affected by the Act\u27s double hull requirement, was premature in claiming that the requirement exacted a regulatory taking of their property. The court used a standard of review that deviated in part from U.S. Supreme Court takings guidelines and instead followed a more conservative script dictated by the U.S. Court of Appeals for the Federal District. This Note will first provide a historical foundation by analyzing the background, statutory content, and intended effect of OPA 90, as well as a short summary of the evolution of takings decisions in both the Supreme Court and the Federal Circuit courts. Following that discussion will be a consideration of the Maritrans opinion itself. This Note concludes with a discussion of the peculiar tailoring of the court\u27s analysis and the effect this opinion may have on both vessel owners and triers of fact in future considerations of OPA 90. This Note will also examine an alternative test of regulatory takings that may have provided guidance for the Maritrans court in reaching its conclusion that OPA 90 did not exact a taking of vessels out of compliance with the Act\u27s construction requirement

Heats of solution in liquid ammonia

Liquid ammonia has properties which make it useful and interesting for making chemical thermodynamic investigations, as had been pointed out by Franklin, and Kluge

Does The Oil Pollution Act Of 1990 Rise To The Level Of A Taking? How The Court Missed The Boat In Maritrans, Inc. v. United States

A recent case in the Court of Federal Claims was the first test by owners of oil tankers and tank barges of section 4115 of the Oil Pollution Act of 1990 (OPA 90), otherwise known as the vessel construction requirement. The Court held that Maritrans, Inc., owners of thirty-seven tank barges affected by the Act\u27s double hull requirement, was premature in claiming that the requirement exacted a regulatory taking of their property. The court used a standard of review that deviated in part from U.S. Supreme Court takings guidelines and instead followed a more conservative script dictated by the U.S. Court of Appeals for the Federal District. This Note will first provide a historical foundation by analyzing the background, statutory content, and intended effect of OPA 90, as well as a short summary of the evolution of takings decisions in both the Supreme Court and the Federal Circuit courts. Following that discussion will be a consideration of the Maritrans opinion itself. This Note concludes with a discussion of the peculiar tailoring of the court\u27s analysis and the effect this opinion may have on both vessel owners and triers of fact in future considerations of OPA 90. This Note will also examine an alternative test of regulatory takings that may have provided guidance for the Maritrans court in reaching its conclusion that OPA 90 did not exact a taking of vessels out of compliance with the Act\u27s construction requirement

Detection of the TCDD binding-fingerprint within the Ah receptor ligand binding domain by structurally driven mutagenesis and functional analysis

The aryl hydrocarbon receptor (AhR) is a ligand-dependent, basic helix-loop-helix Per-Arnt-Sim (PAS)-containing transcription factor that can bind and be activated by structurally diverse chemicals, including the toxic environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Our previous three-dimensional homology model of the mouse AhR (mAhR) PAS B ligand binding domain allowed identification of the binding site and its experimental validation. We have extended this analysis by conducting comparative structural modeling studies of the ligand binding domains of six additional highaffinity mammalian AhRs. These results, coupled with site-directed mutagenesis and AhR functional analysis, have allowed detection of the "TCDD binding-fingerprint" of conserved residues within the ligand binding cavity necessary for high-affinity TCDD binding and TCDD-dependent AhR transformation DNA binding. The essential role of selected residues was further evaluated using molecular docking simulations of TCDD with both wild-type and mutant mAhRs. Taken together, our results dramatically improve our understanding of the molecular determinants of TCDD binding and provide a basis for future studies directed toward rationalizing the observed species differences in AhR sensitivity to TCDD and understanding the mechanistic basis for the dramatic diversity in AhR ligand structure. © 2009 American Chemical Society

Tablet computers in assessing performance in a high stakes exam : opinion matters

The authors would like to thank Dr Craig brown, University of Aberdeen for assistance with data analysis.Peer reviewedPublisher PD

Chemicals having estrogenic activity can be released from some bisphenol a-free, hard and clear, thermoplastic resins

Background: Chemicals that have estrogenic activity (EA) can potentially cause adverse health effects in mammals including humans, sometimes at low doses in fetal through juvenile stages with effects detected in adults. Polycarbonate (PC) thermoplastic resins made from bisphenol A (BPA), a chemical that has EA, are now often avoided in products used by babies. Other BPA-free thermoplastic resins, some hypothesized or advertised to be EA-free, are replacing PC resins used to make reusable hard and clear thermoplastic products such as baby bottles. Methods: We used two very sensitive and accurate in vitro assays (MCF-7 and BG1Luc human cell lines) to quantify the EA of chemicals leached into ethanol or water/saline extracts of fourteen unstressed or stressed (autoclaving, microwaving, UV radiation) thermoplastic resins. Estrogen receptor (ER)-dependent agonist responses were confirmed by their inhibition with the ER antagonist ICI 182,780. Results: Our data showed that some (4/14) unstressed and stressed BPA-free thermoplastic resins leached chemicals having significant levels of EA, including one polystyrene (PS), and three Tritan™ resins, the latter reportedly EA-free. Exposure to UV radiation in natural sunlight resulted in an increased release of EA from Tritan™ resins. Triphenyl-phosphate (TPP), an additive used to manufacture some thermoplastic resins such as Tritan™, exhibited EA in both MCF-7 and BG1Luc assays. Ten unstressed or stressed glycol-modified polyethylene terephthalate (PETG), cyclic olefin polymer (COP) or copolymer (COC) thermoplastic resins did not release chemicals with detectable EA under any test condition. Conclusions: This hazard survey study assessed the release of chemicals exhibiting EA as detected by two sensitive, widely used and accepted, human cell line in vitro assays. Four PC replacement resins (Tritan™ and PS) released chemicals having EA. However, ten other PC-replacement resins did not leach chemicals having EA (EA-free-resins). These results indicate that PC-replacement plastic products could be made from EA-free resins (if appropriate EA-free additives are chosen) that maintain advantages of re-usable plastic items (price, weight, shatter resistance) without releasing chemicals having EA that potentially produce adverse health effects on current or future generations.This work was supported by the following NIH/NIEHS grants: R44 ES011469, 01–03 (CZY); 1R43/44 ES014806, 01–03 (CZY); subcontract (CZY, PI) on an NIH Grant 01–03 43/44ES018083-01. This work was also supported by NIH grants to MSD (P42 ES004699), and DJK and SIY (1R43ES018083-01-03, NIEHS 1R44ES019442-01-03 and NIEHS 2R44ES016964-01-03).Neuroscienc

A Heart And A Rose

https://digitalcommons.library.umaine.edu/mmb-vp/3608/thumbnail.jp