Geographically Apart, Attitudinally Very Close: A Comparison of Attitudes toward Animals between Romania and Mexico City

Among other regions, Romania and Mexico (particularly Mexico City) are often cited as problematic areas in surveys and reports on the growing population of stray animals in urban areas. The aim of our study was to adapt for Romanian and Spanish languages usage of an instrument that includes significant psychological and social dimensions of the attitudes toward animals (i.e., Attitudes toward Animals questionnaire [ATA]; Fehlbaum, Waiblinger, & Turner, 2010; Turner, 2010) and compare these attitudes between two countries that are confronted with similar situations regarding stray animals (dogs and cats). ATA consists of 27 statements on nature conservation, wild animals, farm animals, companion animals, meat eating, and animal feelings and cognition. The original version of ATA was translated into Romanian and Spanish. Data were collected from 295 Romanian respondents (2013–2014) and 302 respondents from Mexico City (2011–2012). Significant differences in the amplitude of the level of agreement or disagreement (not in the direction of the answers) were found between samples in 20 of the 27 items of the questionnaire, most of them regarding the utility and benefits of pet keeping and the attribution of thoughts and emotions to animals (i.e., Romanians had more favorable attitudes than Mexicans). Both samples expressed similar high levels of likeability toward dogs and cats and agreement about humane strategies of pet management, such as painless euthanasia. Stray dogs and cats are perceived as a public problem by both Romanian and Mexican participants, with the specification that cats are significantly seen as more problematic in Mexico City than in Romania

Identifying the Muscle Contraction Activity at Athletes Using Brain Mapping

Background: Understanding the brain function and how it coordinate the motor activity, means to have  a map of brain using a lot of modern technologies which can give us information about the role and functions of different brain areas. Objective: The aim of our study is to explore the brain activity using electroencephalography (EEG) and make the evaluation of differences in brain functions depend on specific sport activity. Methods: We make the study on two lots of athletes from judo (12subjects) and volleyball (11subjects), yrs. 22. All of them have a great sport activity experience and the anthropometric characteristics are similar. For recording the brain activity we use Nihon EEG product. The protocol of the research includes recording of brain activity during muscle contraction and relax of hand flexors. The parameters that we follow up are alpha1, alpha2 and theta waves. The information have been analysed using statistic methods and Pearson coefficient. Judo players present a little bit increase values of theta waves and we observe also a correlation between alpha waves for dominant hemisphere. For the second lot the values of theta waves are highest. Conclusions: Analyse the behaviour of EEG waves could help the trainer and staff for approach the training results in term of build the brain and motor pattern. This is the result of professional approach of training based on neurophysiologic assessment using the brain mapping.</em

Searching for cold-adapted microorganisms in the underground glacier of Scarisoara Ice Cave, Romania

Scarisoara Ice Cave (Romania) hosts one of world’s largest and oldest underground glacier. While no studies were carried out on the existence of microorganisms in this cave’s ice block, our interest is to investigate the presence of microorganisms and their chronological distribution in the cave’s subterranean ice in relationship with past climatic changes. Samples were collected from ice layers of different age (from present to ~900 cal. yrs. BP), and the diversity of embedded microbial communities was assessed by classical cultivation and molecular techniques. The microorganisms from icesediments were cultivated at 4 °C and 15 °C, in the presence and absence of light. Epifluorescence microscopy analysis indicates the presence of autotrophic prokaryotes and eukaryotes in sunlightexposed ice and water samples. Total DNA was isolated from each ice sample and the bacterial and eukaryotic SSU-rRNA genes were amplified by PCR. The chemical composition and organic content of both deeply buried (&gt;10 m inside the ice block) and surface (supra- glacial pond water) habitats were analyzed in relation to their age and organic composition. This study is the first to report on the presence of both prokaryotic and eukaryotic microorganisms in the subterranean ice block of Scarisoara Ice Cave, thriving in both organic-rich ice and clear ice layers. Phototrophic prokaryotes and eukaryotes were identified in sun-exposed recent ice. The composition of cold-adapted ice embedded microbiota varied with the habitat age and organic content, as resulting from dissimilarities in growth curve profiles at two different temperatures. The presence of bacteria and eukaryotes in all the analyzed samples was asserted by PCR amplification of SSU-rRNA gene fragments. These findings can be further used to reconstruct changes in the microbial diversity over the past approximately 5000 years, in correlation with climatic and environmental changes recorded by the ice block

Overdose escalation and the COVID-19 pandemic: An urgent reminder to innovate Canada’s response to the opioid crisis

A ‘dual crisis’ situation in Canada has gained increasing attention in the past months, as deaths by opioid overdose have reached record highs during the COVID-19 pandemic.&nbsp; This review consolidates preliminary literature from January to October 2020 on factors driving the overdose resurgence during the COVID-19 pandemic and interventions which may address them. Converging themes suggest contributions from pandemic-related disruptions to social structure, health risks, and healthcare. These changes have increased both personal and systemic risk factors to overdose, with longstanding gaps in overdose prevention strategies exacerbating their impact. Solutions to address baseline and pandemic-related barriers include novel, evidence-based drug policy changes and logistical adaptations of care to pandemic-imposed restrictions. As more data emerges, rigorous studies are needed to confirm which of the proposed factors are causative, and which interventions demonstrate efficacy. Future systematic reviews will help clarify the relative importance of each and provide more comprehensive guidance

The Development of a Canine Para-Agility Program: Positive Affects in Children with Autism and in Therapy Dogs

Previous research has demonstrated that both children and dogs might benefir from Animal-Assisted Activities (AAA), with some factors mediating/ moderating the results. The present study took these factors into consideration, by creating an AAA program consisting of two types of human-animal interactions (structured and unstructured activities), with an accent being put on the encouragement of positive behaviors. Differences in the frequency of behavioral indicators of positive affects were compared between sessions, in both species (humans and dogs). The preliminary analysis of the results indicated no significant differences between the structured and unstructured sessions in regards of the behavioral indicators of positive affects in humans and dogs, concluding that children and dogs enjoyed the activities in both types of sessions. A more in depth statistical analysis is currently being performed

Pancreatic Adenocarcinoma: Imaging Modalities and the Role of Artificial Intelligence in Analyzing CT and MRI Images

Pancreatic ductal adenocarcinoma (PDAC) stands out as the predominant malignant neoplasm affecting the pancreas, characterized by a poor prognosis, in most cases patients being diagnosed in a nonresectable stage. Image-based artificial intelligence (AI) models implemented in tumor detection, segmentation, and classification could improve diagnosis with better treatment options and increased survival. This review included papers published in the last five years and describes the current trends in AI algorithms used in PDAC. We analyzed the applications of AI in the detection of PDAC, segmentation of the lesion, and classification algorithms used in differential diagnosis, prognosis, and histopathological and genomic prediction. The results show a lack of multi-institutional collaboration and stresses the need for bigger datasets in order for AI models to be implemented in a clinically relevant manner

Searching for Cold-Adapted Microorganisms in the Underground Glacier of Scarisoara Ice Cave, Romania

Scarisoara Ice Cave (Romania) hosts one of world’s largest and oldest underground glacier. While no studies were carried out on the existence of microorganisms in this cave’s ice block, our interest is to investigate the presence of microorganisms and their chronological distribution in the cave’s subterranean ice in relationship with past climatic changes. Samples were collected from ice layers of different age (from present to ~900 cal. yrs. BP), and the diversity of embedded microbial communities was assessed by classical cultivation and molecular techniques. The microorganisms from icesediments were cultivated at 4 °C and 15 °C, in the presence and absence of light. Epifluorescence microscopy analysis indicates the presence of autotrophic prokaryotes and eukaryotes in sunlightexposed ice and water samples. Total DNA was isolated from each ice sample and the bacterial and eukaryotic SSU-rRNA genes were amplified by PCR. The chemical composition and organic content of both deeply buried (\u3e10 m inside the ice block) and surface (supra-glacial pond water) habitats were analyzed in relation to their age and organic composition. This study is the first to report on the presence of both prokaryotic and eukaryotic microorganisms in the subterranean ice block of Scarisoara Ice Cave, thriving in both organic-rich ice and clear ice layers. Phototrophic prokaryotes and eukaryotes were identified in sun-exposed recent ice. The composition of cold-adapted ice embedded microbiota varied with the habitat age and organic content, as resulting from dissimilarities in growth curve profiles at two different temperatures. The presence of bacteria and eukaryotes in all the analyzed samples was asserted by PCR amplification of SSU-rRNA gene fragments. These findings can be further used to reconstruct changes in the microbial diversity over the past approximately 5000 years, in correlation with climatic and environmental changes recorded by the ice bloc

The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes

Ozone is an allotropic form of oxygen, so in the medical field ozone therapy has special effects. Starting from the premise that bio-oxidative ozone therapy reduces the number of bacteria, in the present study two approaches were proposed: to evaluate the biological effects of ozone gas on the tooth enamel remineralization process and to demonstrate its impact on the morphology and confluence of human primary gingival cells, namely keratinocytes (PGK) and fibroblasts (HGF). The ozone produced by HealOzone was applied in vivo to 68 M1s (first permanent molars), both maxillary and mandibular, on the occlusal surfaces at pit and fissure. The molars included in the study recorded values between 13 and 24 according to the DIAGNOdent Pen 2190 scale, this being the main inclusion/exclusion criterion for the investigated molars. Because the gas can make contact with primary gingival cells during the ozonation process, both human gingival fibroblasts and keratinocytes were exposed to different doses of ozone (20 s, 40 s, 60 s), and its effects were observed with the Olympus IX73 inverted microscope. The contact of ozone with the human primary gingival cells demonstrates cell sensitivity to the action of ozone, this being higher in fibroblasts compared to keratinocytes, but it is not considered toxic because all the changes are reversible at 48 h after exposure