Broadly conserved roles of TMEM131 family proteins in intracellular collagen assembly and secretory cargo trafficking.

Collagen is the most abundant protein in animals. Its dysregulation contributes to aging and many human disorders, including pathological tissue fibrosis in major organs. How premature collagen proteins in the endoplasmic reticulum (ER) assemble and route for secretion remains molecularly undefined. From an RNA interference screen, we identified an uncharacterized Caenorhabditis elegans gene tmem-131, deficiency of which impairs collagen production and activates ER stress response. We find that amino termini of human TMEM131 contain bacterial PapD chaperone-like domains, which recruit premature collagen monomers for proper assembly and secretion. Carboxy termini of TMEM131 interact with TRAPPC8, a component of the TRAPP tethering complex, to drive collagen cargo trafficking from ER to the Golgi. We provide evidence that previously undescribed roles of TMEM131 in collagen recruitment and secretion are evolutionarily conserved in C. elegans, Drosophila, and humans

Percutaneous transthoracic catheter drainage prior to surgery in treating neonates with congenital macrocystic lung malformation presenting with respiratory distress

BackgoundIt is rarely seen that neonates with congenital macrocystic lung malformation (CMLM) presenting with respiratory distress require emergency intervention. No consensus has been achieved concerning the best policy facing such condition. This study aims to evaluate the efficacy and safety of our strategies in treating neonates with CMLM presenting with respiratory distress.MethodsWe retrospectively reviewed the data of six neonates with CMLM presenting with respiratory distress from April 2020 to October 2022 for whom drainage-prior-to-surgery strategy were adopted and favorable outcomes were obtained. The relevant data was reviewed and analyzed.ResultsAll the patients were prenatally diagnosed with congenital lung malformation and postnatally as congenital macrocystic lung malformation via CT scan. Each neonate accepted percutaneous thoracic catheter drainage prior to surgery. The first and fifth neonates with macrocystic lung mass experienced prompt open lobectomy and delayed thoracoscopic surgery due to failure of air drainage, respectively. The other four patients obtained good drainage of the large air-filled cyst, thus gaining the opportunity for elective thoracoscopic surgery within median 45 days.ConclusionsFor neonates with macrocystic lung malformation presenting with respiratory distress due to mediastinal compression, percutaneous thoracic catheter drainage is worth a shot for elective thoracoscopic surgery due to its feasibility and safety

Multifunctional ytterbium oxide buffer for perovskite solar cells

Perovskite solar cells (PSCs) comprise a solid perovskite absorber sandwiched between several layers of different charge-selective materials, ensuring unidirectional current flow and high voltage output of the devices. A ‘buffer material’ between the electron-selective layer and the metal electrode in p-type/intrinsic/n-type (p-i-n) PSCs (also known as inverted PSCs) enables electrons to flow from the electron-selective layer to the electrode. Furthermore, it acts as a barrier inhibiting the inter-diffusion of harmful species into or degradation products out of the perovskite absorber. Thus far, evaporable organic molecules and atomic-layer-deposited metal oxides have been successful, but each has specific imperfections. Here we report a chemically stable and multifunctional buffer material, ytterbium oxide (YbOx), for p-i-n PSCs by scalable thermal evaporation deposition. We used this YbOx buffer in the p-i-n PSCs with a narrow-bandgap perovskite absorber, yielding a certified power conversion efficiency of more than 25%. We also demonstrate the broad applicability of YbOx in enabling highly efficient PSCs from various types of perovskite absorber layer, delivering state-of-the-art efficiencies of 20.1% for the wide-bandgap perovskite absorber and 22.1% for the mid-bandgap perovskite absorber, respectively. Moreover, when subjected to ISOS-L-3 accelerated ageing, encapsulated devices with YbOx exhibit markedly enhanced device stability

Aerodynamic Characteristics When Trains Pass Each Other in High-Speed Railway Shield Tunnel

The characteristics of the aerodynamic effects of high-speed trains passing in a shield tunnel were studied using the three-dimensional, compressible, unsteady Reynolds-averaged Navier-Stokes (RANS) equations for the simulation analysis. Numerical calculations were compared with dynamic model tests to verify the reliability of the numerical simulations. The results showed that the compression wave characteristics of high-speed trains in shield tunnels were consistent with those in molded concrete tunnels. When high-speed trains met in the middle of the shield tunnel, the positive and negative peak attenuation rates of shield tunnels were higher than the positive and negative peak attenuation rates of molded lining tunnels, and the maximum pressure attenuation rate could reach 57.8%. At the same time, the micro-pressure wave of the former was reduced by 10.78%, compared with those of the latter. When meeting cars at different locations, the maximum pressure at the intersection in the center of the tunnel was significantly higher than those at other intersection points in the tunnel. Different intersection positions and different tunnel lining structures had relatively little influence on the aerodynamic drag and lateral force, while train speed had a significant influence

Testing and Analysis of the Vibration Response Characteristics of Heavy-Haul Railway Tunnels and Surrounding Soil with Base Voids

This paper discusses research on the dynamic response characteristics of a heavy-haul railway tunnel and the surrounding soil under the conditions of substrate health and a base void. The detection results of the base condition of 20 double-track tunnels for a heavy-haul railway show the main distribution law of base voids. Based on this, a 1:20 scale test model of a heavy-haul railway tunnel is established. The vibration load of the train is established by a vibration exciter arranged at the tunnel invert. The dynamic response and attenuation law of a heavy-haul railway tunnel lining structure and the surrounding soil are tested using acceleration sensors, strain gauges, and soil pressure boxes. The research results show that most of the diseases are concentrated below the heavy-haul line. The base void causes the peak acceleration of the nearby tunnel invert to increase by 55.6%. Tunnel annular construction joints reduce the conductivity of the vibration waves in the axial direction of the tunnel. The acceleration attenuation rate of the soil above the tunnel invert is significantly less than that under the invert. The base void reduces the acceleration of the nearby soil layer by 19.4% and increases the stress on the surface of the nearby tunnel invert by 21.3%, and the stress change amplitude increases by 0.55%. The tunnel structure in the area of the base void experiences fatigue damage. The base void causes the compaction and bearing capacity of the nearby soil to decrease and the softening speed of the tunnel basement soil layer to increase. Therefore, for the basement damage to heavy-haul railway tunnels, “early detection, early treatment” should be performed

Analysis of the Causes of the Collapse of a Deep-Buried Large Cross-Section of Loess Tunnel and Evaluation of Treatment Measures

To address the problem of the collapse of the roof of the Bailuyuan tunnel during construction, the causes of collapse were analyzed, targeted treatment measures were proposed, and the effects of the treatment measures were evaluated through on-site monitoring and three-dimensional numerical simulations. The results showed that the particular characteristics of loess and the synergy of groundwater were the internal causes of the tunnel’s collapse as well as, to a certain extent, atmospheric precipitation. Therefore, the combination of multiple factors contributed to the tunnel’s collapse. Untimely monitoring and measurement, as well as the low initial support parameters, reflect a lack of human understanding of the collapse. Based on the analysis of the causes of the collapse, comprehensive treatment measures for inside and outside the tunnel are proposed, which are shown to be effective and to be capable of preventing the occurrence of further collapses. After the collapse treatment, the measured maximum settlement of the tunnel vault was 65.1 mm, the maximum horizontal convergence was 25 mm, the maximum surrounding rock pressure was 0.56 MPa, and the maximum stress on the steel arch frame was 54.34 MPa. Compared with the original design plan, the vertical stress, horizontal stress, and shear stress of the surrounding rock obtained from numerical simulation after the collapse treatment were greatly reduced, the reduction rate at the vault reached 50%, and the safety factors of the initial support positions after treatment met the specification requirements. The research results can provide engineering guidance for the design and construction of large-section tunnels crossing deep-loess strata, and they are of important engineering significance