Chromosome-level genome assembly of the pygmy grasshopper Eucriotettix oculatus (Orthoptera: Tetrigoidea)

Abstract The pygmy grasshoppers, which belong to the superfamily Tetrigoidea, exhibit remarkable environmental adaptability. However, no study has yet reported a reference genome for this group. In this study, we assembled a high-quality chromosome-scale genome of Eucriotettix oculatus, which survive in the environment heavily polluted by heavy metals, achieved through Illumina and PacBio sequencing, alongside chromosome conformation capture techniques. The resulting genome spans 985.45 Mb across seven chromosomes (range: 71.55 to 266.65 Mb) and features an N50 length of 123.82 Mb. Chr5 is considered to be the single sex chromosome (X). This genome is composed of 46.42% repetitive elements and contains 14,906 predicted protein-coding genes, 91.63% of which are functionally annotated. Decoding the E. oculatus genome not only promotes future studies on environmental adaptation for the pygmy grasshopper, but also provides valuable resources for in-depth investigation on phylogeny, evolution, and behavior of Orthoptera

Research on the propagation of laser beams array with coherent and incoherent combination in dynamic atmosphere turbulence

High-power laser systems mostly use coherent or incoherent combined beams to achieve higher laser output power to satisfy the application. However, the far-field beam quality of laser will be reduced when propagating over atmosphere. Based on the propagation model of laser beams array, we use atmospheric coherence length, laser duration and average wind velocity to construct dynamic atmospheric turbulence which is characterized as a phase screen sequence. Meanwhile, considered as the indexes to evaluate beam quality, peak intensity and intensity in bucket are comparatively analysed in coherent and incoherent combined beams in far-field. The results indicate that in weaker turbulence circumstances, coherent combined beam has an advantage compared with the incoherent combined beams when laser duration is short, and coherent combination is more suitable for pulsed laser. With laser duration is increasing, the beam quality of incoherent and coherent combined beams both decrease and tend to be close. In stronger turbulence circumstances, the corresponding laser duration will be shorter when the beam quality of coherent combined beams is extraordinary close to that of incoherent combined beams. The researches can provide important data for high-power laser system to select the optimal beam combination mode to improve its performance

Multidomain load balancing

This paper investigates dynamic load balancing issues in the multidomain environment where local area networks (LANs) are interconnected by the Internet. Because of the much slower Internet communication speed and limited bandwidth, existing load balancing algorithms for LANs are unsuitable for the multidomain environment. New issues such as lag time in updating load information and network cost of transferring jobs must be addressed. To tackle these problems, the conventional least load scheduler is extended to the multidomain environment by employing a hierarchical structure, and several quick update techniques are proposed. Also, a heuristic taking both the machine load and the network cost into consideration is developed to evaluate the benefits of sending jobs to computers in different domains. A set of experiments conducted on the BALANCE testbed showed that the proposed techniques provide significant performance improvement over existing algorithms

A new gamboge derivative Compound 2 inhibits cancer stem‐like cells via

Cancer stem-like cells represent a population of tumour-initiating cells that lead to the relapse and metastasis of cancer. Conventional anti-cancer therapeutic drugs are usually ineffective in eliminating the cancer stem-like cells. Therefore, new drugs or therapeutic methods effectively targeting cancer stem-like cells are in urgent need to successfully cure cancer. Gamboge is a natural anti-cancer medicine whose pharmacological effects are different from those of conventional chemotherapeutical drugs and they can kill some kinds of cancer cells selectively. In this study, we identified a new gamboge derivative, Compound 2 (C2), which presents eminent suppression effects on cancer cells. Interestingly, when compared with cisplatin (CDDP), C2 effectively suppresses the growth of both cancer stem-like cells and non-cancer stem-like cells derived from head and neck squamous cell carcinoma (HNSCC), inhibiting the formation of tumour spheres and colony in vitro, resulting in the loss of expression of multiple cancer stem cell (CSC)-related molecules in HNSCC. Treating with C2 effectively inhibited the growth of HNSCC in BALB/C nude mice. Further investigation found that C2 notably inhibits the activation of epithelial growth factor receptor and the phosphorylation of its downstream protein kinase homo sapiens v-akt murine thymoma viral oncogene homolog (AKT) in HNSCC, resulting in down-regulation of multiple CSC-related molecules in HNSCC. Our study has demonstrated that C2 effectively inhibits the stem-like property of cancer stem-like cells in HNSCC and may be a hopeful targeting drug in cancer therapy

A cancer-associated fibroblast subtypes-based signature enables the evaluation of immunotherapy response and prognosis in bladder cancer

Summary: Bladder cancer (BLCA) is one of the most prevalent and heterogeneous urinary malignant tumors. Previous researches have reported a significant association between cancer-associated fibroblasts (CAFs) and poor prognosis of tumor patients. However, uncertainty surrounds the role of CAFs in the BLCA tumor microenvironment, necessitating further investigation into the CAFs-related gene signatures in BLCA. In this study, we identified three CAF subtypes in BLCA according to single-cell RNA-seq data and constructed CAFs-related risk score (CRRS) by screening 102,714 signatures. The survival analysis, ROC curves, and nomogram suggested that CRRS was a valuable predictor in 2,042 patients from 9 available public datasets and Xiangya real-world cohort. We further revealed the significant correlation between CRRS and clinicopathological characteristics, genome alterations, and epithelial-mesenchymal transition (EMT). A high CRRS indicated a non-inflamed phenotype and a lower remission rate of immunotherapy in BLCA. In conclusion, the CRRS had the potential to predict the prognosis and immunotherapy response of BLCA patients