Exploring Disentangled Content Information for Face Forgery Detection

Convolutional neural network based face forgery detection methods have achieved remarkable results during training, but struggled to maintain comparable performance during testing. We observe that the detector is prone to focus more on content information than artifact traces, suggesting that the detector is sensitive to the intrinsic bias of the dataset, which leads to severe overfitting. Motivated by this key observation, we design an easily embeddable disentanglement framework for content information removal, and further propose a Content Consistency Constraint (C2C) and a Global Representation Contrastive Constraint (GRCC) to enhance the independence of disentangled features. Furthermore, we cleverly construct two unbalanced datasets to investigate the impact of the content bias. Extensive visualizations and experiments demonstrate that our framework can not only ignore the interference of content information, but also guide the detector to mine suspicious artifact traces and achieve competitive performance

The Utilization of Rice Blast Resistance Genes in Hybrid Rice Breeding in China

Hybrid rice has demonstrated promises of yield gain for over several decades since its conception and massive deployment in China. One of the common bottlenecks of hybrid rice is the availability of suitable breeding lines as parents to produce marketable rice grains. Due to limitation of genetic diversity of breeding parent, hybrid rice is extremely vulnerable to rice blast disease caused by the fungal pathogen Magnaporthe oryzae. M. oryzae is a highly adaptive fungus that often gains new virulence to reduce crop resistance resulting in massive yield loss and crop failure. To secure yield gain of hybrid rice, identification and integration of diverse sources of resistance genes into hybrid rice are super critical. In this chapter, we will present strategies to identify, characterize, and stack effective blast resistance genes in hybrid rice breeding in China

Application and Development of CRISPR/Cas9 Technology in Pig Research

Pigs provide valuable meat sources, disease models, and research materials for humans. However, traditional methods no longer meet the developing needs of pig production. More recently, advanced biotechnologies such as SCNT and genome editing are enabling researchers to manipulate genomic DNA molecules. Such methods have greatly promoted the advancement of pig research. Three gene editing platforms including ZFNs, TALENs, and CRISPR/Cas are becoming increasingly prevalent in life science research, with CRISPR/Cas9 now being the most widely used. CRISPR/Cas9, a part of the defense mechanism against viral infection, was discovered in prokaryotes and has now developed as a powerful and effective genome editing tool that can introduce and enhance modifications to the eukaryotic genomes in a range of animals including insects, amphibians, fish, and mammals in a predictable manner. Given its excellent characteristics that are superior to other tailored endonucleases systems, CRISPR/Cas9 is suitable for conducting pig-related studies. In this review, we briefly discuss the historical perspectives of CRISPR/Cas9 technology and highlight the applications and developments for using CRISPR/Cas9-based methods in pig research. We will also review the choices for delivering genome editing elements and the merits and drawbacks of utilizing the CRISPR/Cas9 technology for pig research, as well as the future prospects

Study on the Creep Characteristics of Sandstone under Coupled Stress-water Pressure

Long-term interaction between stress and water pressure leads to creep damage of reservoir bank slope. As a result there will be instability of the bank slopes in many water conservancy projects. The rock mass creeping effect of coupled stress-water pressure was studied by using a typical sandstone rock from the Three Gorges reservoir area. The experiment was conducted by using the rock immersion-air-drying cyclic load rheometer device (designed and manufactured by our research team). Based on the experimental results, the following key points were observed: 1) the creep strain and the steady-state creep rate was increasing when the water pressure increased (at the same stress level). Under the same water pressure, the increase in the axial pressure resulted in the increase in the creep strain and steady creep rate of the sandstone specimens. 2) the increase in the axial pressure increased the creep strain and steady-state creep rate of the sandstone specimens while the water pressure increased. The mechanical properties of the sandstone specimens were affected by the water pressure. 3) the water infiltrates through the pore surfaces. As a result, the rate of deformation will increase while the bearing capacity and long-term strength of the rock decrease. This paper provides a solid theoretical foundation for the evaluation and prediction of reservoir geological hazards

Study on the Time-lag Failure of Sandstone With Different Degrees of Unloading Damage

The unloading effect of rock mass excavation is an inevitable practice, and it’s often characterized by a relatively large-scale engineering hazard with a noticeable time lag.A set of unloading triaxial tests were conducted on a sandstone rock to establish the deformation law and the threshold time. Based on the renormalization group theory, the unloading sandstone model was developed by considering the interaction between particles. Similarly, a logistic model was used to predict the unloading damage of sandstone. The unloading time lag damage of sandstone rock was predicted by using the damage threshold. The research shows that: (1) The higher the degree of unloading, the shorter the time-lag failure. (2) The damage range of critical values was optimized. (3) The error between the predicted value and the experimental value of the time threshold was almost less than 5 %, the prediction result was found to be good, and the employed logistic evolution model was reasonable. The findings of this research provide a prediction method and precise information about the mechanism of unloading time lag deformation. Therefore, it can be used as a reference for excavation-support design of underground structures

Effect of the Water-Rock Interaction on the Creep Mechanical Properties of the Sandstone Rock

After the commencement of the Three Gorges hydropower project, the reservoir water level has been fluctuating by 30 m (145–175 m) annually. The stability of the bank slope has been highlighted since the reservoir water level has been repeated. Apart from that, the long-term effect of the water-rock interaction on the rheological and mechanical properties of the rock was not studied sufficiently. Therefore, a typical sandstone rock was brought from the Three Gorges reservoir area, to meet the purpose of this study. Then, a series of water-rock interaction tests were conducted to simulate the fluctuations in the reservoir water level. Based upon the experimental results, the following points were pointed out: 1) for the first three successive water-rock interaction cycles, the long-term strength of the rock was dramatically reduced. In contrast, the rate of reduction on the long-term strength of the rock was getting a steady state after six successive water rock interactions.2) At the failure stress level, the rock specimens exhibited similar characteristics under different water-rock interaction cycles. 3) The densely compacted micro structures of the sandstone rock were transformed into loose and porous state

New Method for Determination of Residual Strength Parameters and Critical Damage Value

Residual strength and critical damage parameters are worthy to evaluate the stability of engineered rock masses. In this paper, new thinking, repeated load test on a single specimen was proposed to measure the residual strength of the rock. And author proposed to modify the critical damage value based on residual constitutive energy. The test results showed that: (1) the residual strength of rock is mainly controlled by the confining pressure, without a clear relationship with the confining pressure and stress path of the initial loading failure. (2) The residual strength parameters of the rock specimens under repeated loading test are consistent with the conventional triaxial test. Most importantly, the proposed method is relatively less dispersion, cheap, reliable, and time-saving. (3) The corrected critical damage value was reasonable. Relevant test methods can provide a useful reference for the determination of residual strength parameters and critical damage value

Insolation driven biomagnetic response to the Holocene Warm Period in semi-arid East Asia

The Holocene Warm Period (HWP) provides valuable insights into the climate system and biotic responses to environmental variability and thus serves as an excellent analogue for future global climate changes. Here we document, for the first time, that warm and wet HWP conditions were highly favourable for magnetofossil proliferation in the semi-arid Asian interior. The pronounced increase of magnetofossil concentrations at ~9.8 ka and decrease at ~5.9 ka in Dali Lake coincided respectively with the onset and termination of the HWP, and are respectively linked to increased nutrient supply due to postglacial warming and poor nutrition due to drying at ~6 ka in the Asian interior. The two-stage transition at ~7.7 ka correlates well with increased organic carbon in middle HWP and suggests that improved climate conditions, leading to high quality nutrient influx, fostered magnetofossil proliferation. Our findings represent an excellent lake record in which magnetofossil abundance is, through nutrient availability, controlled by insolation driven climate changes.This research was supported by the NSFC grant 41330104 and the 973 program grant 2012CB821900. J.X. was supported by the 973 program grant 2010CB833400 and the NSFC grant 41130101. J.L. received support from the NSFC grant 41374004. C.D. acknowledges further support from the NSFC grant 40925012 and the CAS Bairen Program

Applications of CRISPR/Cas Technology to Research the Synthetic Genomics of Yeast

The whole genome projects open the prelude to the diversity and complexity of biological genome by generating immense data. For the sake of exploring the riddle of the genome, scientists around the world have dedicated themselves in annotating for these massive data. However, searching for the exact and valuable information is like looking for a needle in a haystack. Advances in gene editing technology have allowed researchers to precisely manipulate the targeted functional genes in the genome by the state-of-the-art gene-editing tools, so as to facilitate the studies involving the fields of biology, agriculture, food industry, medicine, environment and healthcare in a more convenient way. As a sort of pioneer editing devices, the CRISPR/Cas systems having various versatile homologs and variants, now are rapidly giving impetus to the development of synthetic genomics and synthetic biology. Firstly, in the chapter, we will present the classification, structural and functional diversity of CRISPR/Cas systems. Then we will emphasize the applications in synthetic genome of yeast (Saccharomyces cerevisiae) using CRISPR/Cas technology based on year order. Finally, the summary and prospection of synthetic genomics as well as synthetic biotechnology based on CRISPR/Cas systems and their further utilizations in yeast are narrated

Cell transcriptomic atlas of the non-human primate Macaca fascicularis.

Studying tissue composition and function in non-human primates (NHPs) is crucial to understand the nature of our own species. Here we present a large-scale cell transcriptomic atlas that encompasses over 1 million cells from 45 tissues of the adult NHP Macaca fascicularis. This dataset provides a vast annotated resource to study a species phylogenetically close to humans. To demonstrate the utility of the atlas, we have reconstructed the cell-cell interaction networks that drive Wnt signalling across the body, mapped the distribution of receptors and co-receptors for viruses causing human infectious diseases, and intersected our data with human genetic disease orthologues to establish potential clinical associations. Our M. fascicularis cell atlas constitutes an essential reference for future studies in humans and NHPs.We thank W. Liu and L. Xu from the Huazhen Laboratory Animal Breeding Centre for helping in the collection of monkey tissues, D. Zhu and H. Li from the Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) for technical help, G. Guo and H. Sun from Zhejiang University for providing HCL and MCA gene expression data matrices, G. Dong and C. Liu from BGI Research, and X. Zhang, P. Li and C. Qi from the Guangzhou Institutes of Biomedicine and Health for experimental advice or providing reagents. This work was supported by the Shenzhen Basic Research Project for Excellent Young Scholars (RCYX20200714114644191), Shenzhen Key Laboratory of Single-Cell Omics (ZDSYS20190902093613831), Shenzhen Bay Laboratory (SZBL2019062801012) and Guangdong Provincial Key Laboratory of Genome Read and Write (2017B030301011). In addition, L.L. was supported by the National Natural Science Foundation of China (31900466), Y. Hou was supported by the Natural Science Foundation of Guangdong Province (2018A030313379) and M.A.E. was supported by a Changbai Mountain Scholar award (419020201252), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16030502), a Chinese Academy of Sciences–Japan Society for the Promotion of Science joint research project (GJHZ2093), the National Natural Science Foundation of China (92068106, U20A2015) and the Guangdong Basic and Applied Basic Research Foundation (2021B1515120075). M.L. was supported by the National Key Research and Development Program of China (2021YFC2600200).S