Planning Emergency Shelters for Urban Disasters: A Multi-Level Location–Allocation Modeling Approach

In recent years, cities are threatened by various natural hazards. Planning emergency shelters in advance is an effective approach to reducing the damage caused by disasters and ensuring the safety of residents. Thus, providing the optimal layout of urban emergency shelters is an important stage of disaster management and an act of humanitarian logistics. In order to study the optimal layout of emergency shelters in small mountain cities, this paper constructs multi-level location models for different grades of emergency shelters so as to minimize the travel and construction costs and maximize the coverage rate. Specifically, the actual service of emergency shelters is determined using Geographic Information System (GIS) software and Weighted Voronoi Diagram (WVD) models under the limitation of site capacity, and the space layout is adjusted through combining the actual urban land with the construction position. In this paper, the Jianchuan county seat at Yunnan Province, China, was considered as a case study to illustrate the models of emergency shelters in which the feasibility of the presented models is verified. The proposed research methods and models have provided theoretical basis and a benchmark for the optimal layout of emergency shelters in other small mountain cities

The association between type 2 diabetes and pulmonary cavitation revealed among IGRA-positive tuberculosis patients

The co-occurrence of tuberculosis (TB) and diabetes mellitus (DM) presents a significant obstacle to TB eradication. Pulmonary cavitation can occur in severe cases of TB, particularly in patients with DM. From 1 May 2014 through 30 June 2019, we conducted a cross-sectional study of 1,658 smear- or culture-confirmed pulmonary TB (PTB) patients at the Second Department of Pulmonary Medicine and Tuberculosis, Shenzhen, China. A total of 861 participants who satisfied the criteria (chest CT scan for cavitation, interferon-gamma release assay (IGRA), diagnosis of diabetes mellitus), with the median age of 36.7 years, 63.6% of male, 79.7% IGRA positive, 13.8% with diabetes, and 40.8% with pulmonary cavitation, were included in the study. The association between diabetes and pulmonary cavitation was confirmed in these TB patients (adjusted OR, 2.54; 95% CI, 1.66–3.94; p < 0.001). No associations were observed between diabetes and IGRA, as well as between lung cavitary and IGRA. Based on the criteria of IGRA+/–, pulmonary cavitation+/–, and DM+/–, the further analysis with univariate and multivariate logistic regression were conducted in six subgroups. The significant association between diabetes and pulmonary cavitation was further confirmed in the IGRA+ subgroup (adjusted OR, 3.07; 95% CI, 1.86–5.16; p < 0.001) but not observed in IGRA- individuals. This observation suggests that different immunological mechanisms of pulmonary cavitary/DM may be employed in IGRA+ TB patients from IGRA- TB patients

Mycobacterium tuberculosis -Induced Upregulation of the COX-2/mPGES-1 Pathway in Human Macrophages Is Abrogated by Sulfasalazine

Macrophages are the primary human host cells of intracellular Mycobacterium tuberculosis ( M.tb ) infection, where the magnitude of inflammatory reactions is crucial for determining the outcome of infection. Previously, we showed that the anti-inflammatory drug sulfasalazine (SASP) significantly reduced the M.tb bactericidal burden and histopathological inflammation in mice. Here, we asked which genes in human inflammatory macrophages are affected upon infection with M.tb and how would potential changes impact the functional state of macrophages. We used a flow cytometry sorting system which can distinguish the dead and alive states of M.tb harbored in human monocyte-derived macrophages (MDM). We found that the expression of cyclooxygenase-2 and microsomal prostaglandin E 2 synthase (mPGES)-1 increased significantly in tagRFP + MDM which were infected with alive M.tb . After exposure of polarized M1-MDM to M.tb (H37Rv strain)-conditioned medium (MTB-CM) or to the M.tb -derived 19-kD antigen, the production of PGE 2 and pro-inflammatory cytokines increased 3- to 4-fold. Upon treatment of M1-MDM with SASP, the MTB-CM-induced expression of COX-2 and the release of COX products and cytokines decreased. Elevation of PGE 2 in M1-MDM upon MTB-CM stimulation and modulation by SASP correlated with the activation of the NF-κB pathway. Together, infection of human macrophages by M.tb strongly induces COX-2 and mPGES-1 expression along with massive PGE 2 formation which is abrogated by the anti-inflammatory drug SASP

Planning Emergency Shelters for Urban Disasters: A Multi-Level Location–Allocation Modeling Approach

In recent years, cities are threatened by various natural hazards. Planning emergency shelters in advance is an effective approach to reducing the damage caused by disasters and ensuring the safety of residents. Thus, providing the optimal layout of urban emergency shelters is an important stage of disaster management and an act of humanitarian logistics. In order to study the optimal layout of emergency shelters in small mountain cities, this paper constructs multi-level location models for different grades of emergency shelters so as to minimize the travel and construction costs and maximize the coverage rate. Specifically, the actual service of emergency shelters is determined using Geographic Information System (GIS) software and Weighted Voronoi Diagram (WVD) models under the limitation of site capacity, and the space layout is adjusted through combining the actual urban land with the construction position. In this paper, the Jianchuan county seat at Yunnan Province, China, was considered as a case study to illustrate the models of emergency shelters in which the feasibility of the presented models is verified. The proposed research methods and models have provided theoretical basis and a benchmark for the optimal layout of emergency shelters in other small mountain cities

Discriminating Active Tuberculosis from Latent Tuberculosis Infection by flow cytometric measurement of CD161-expressing T cells

Interferon-gamma Release Assays (IGRAs) significantly increases the possibility for early diagnosis of tuberculosis, but IGRAs alone cannot discriminate active TB from LTBI. Therefore, fast and reliable discrimination of active tuberculosis, especially bacteriology negative tuberculosis, from LTBI is a great necessity. Here we established an assay based on flow cytometric multiparameter assay assessing expression of CD161 along with CD3, CD4, and CD8, whereby a set of indices formulated by the percentages of CD3(+)CD161(+), CD3(+)CD4(+)CD161(+) and CD3(+)CD8(+)CD161(+) T cells multiplied with lymphocyte/monocyte ratio were established. Application of the CD3(+)CD8(+)CD161(+) index to compare a cohort of active tuberculosis with a cohort of LTBI or health control yielded 0.7662 (95% confidence interval [CI] 0.6559-0.8552) or 0.7922 (95% CI 0.6846-0.8763) for sensitivity and 0.9048 (95% CI 0.8209-0.9580) or 0.8939 (95% CI 0.8392-0.9349) for specificity when the TB cohort was AFB(+); the corresponding results were 0.7481 (95% CI 0.6648-0.8198) or 0.7557 (95% CI 0.6730-0.8265) for sensitivity and 0.8571 (95% CI 0.7637-0.9239) or 0.8603 (95% CI 0.8008-0.9075) for specificity when the TB cohort was AFB(-). Our results reveal that in combination with IGRAs, CD161-based indices provide a novel, fast diagnostic solution addressing the limitation of current tuberculosis diagnostics

How Can Cities Respond to Flood Disaster Risks under Multi-Scenario Simulation? A Case Study of Xiamen, China

Flood disasters often have serious impacts on cities. Disaster prevention and mitigation schemes for flood disasters must be based on risk assessment. We constructed an indicator system for flood disaster risk assessment from the aspects of hazard factors, sensitivity to the environment, disaster vulnerability, flood disaster prevention, and resilience. Then we add the precipitation factor as a scenario parameter to the assessment of flood disasters, in order to assess the flood disaster risk under annual average precipitation scenarios, multi-year flood season average precipitation scenarios, and large typhoon precipitation scenarios. Xiamen is one of the cities with more serious flood disasters. We select Xiamen as an example and refer to existing indicators of flood disaster assessment. The results show that: (1) the coefficient of variation of flood disasters in Xiamen under the impact of large-scale typhoon precipitation is large; (2) the drainage and flood control capacity of Xiamen is generally insufficient, and the risk in the old city is high; (3) there are many flood-prone locations in Xiamen. Underpass interchanges, underground spaces, and urban villages have become the new key areas for flood control; and (4) the flood risk in the northern mountainous areas of Xiamen is the highest. Based on the assessment results, we further delineate the urban flood control zones and propose corresponding countermeasures. The study expands the research on flood disaster risk assessment, and also provides reference for relevant cities to deal with flood disasters

How Can Cities Respond to Flood Disaster Risks under Multi-Scenario Simulation? A Case Study of Xiamen, China

Flood disasters often have serious impacts on cities. Disaster prevention and mitigation schemes for flood disasters must be based on risk assessment. We constructed an indicator system for flood disaster risk assessment from the aspects of hazard factors, sensitivity to the environment, disaster vulnerability, flood disaster prevention, and resilience. Then we add the precipitation factor as a scenario parameter to the assessment of flood disasters, in order to assess the flood disaster risk under annual average precipitation scenarios, multi-year flood season average precipitation scenarios, and large typhoon precipitation scenarios. Xiamen is one of the cities with more serious flood disasters. We select Xiamen as an example and refer to existing indicators of flood disaster assessment. The results show that: (1) the coefficient of variation of flood disasters in Xiamen under the impact of large-scale typhoon precipitation is large; (2) the drainage and flood control capacity of Xiamen is generally insufficient, and the risk in the old city is high; (3) there are many flood-prone locations in Xiamen. Underpass interchanges, underground spaces, and urban villages have become the new key areas for flood control; and (4) the flood risk in the northern mountainous areas of Xiamen is the highest. Based on the assessment results, we further delineate the urban flood control zones and propose corresponding countermeasures. The study expands the research on flood disaster risk assessment, and also provides reference for relevant cities to deal with flood disasters

Climate-induced spatial mismatch may intensify giant panda habitat loss and fragmentation

Climate change may disrupt the spatial matching of interacting species, thus compromise the persistence of species. Giant pandas form specialized trophic interactions with bamboo, and empirical evidence indicated that climate in panda distribution range has warmed during the past half-century. A critical effort to reduce giant panda extinction risk is to measure the stability state of panda-bamboo and detect the trends of recent change. However, most studies projected what would happen only for a single component of the panda-bamboo system under future climate scenarios. Little has been known about whether climate warming has decoupled spatial matching between giant panda and bamboo. Here, we investigated the spatial matching between giant panda and bamboo, and explored the extent to which the panda thermally suitable habitats shifted and fragmented over the past half-century. We found that giant pandas gained 4750 km(2) of thermally suitable areas. However, the thermally suitable habitat shrank by 8.2%, and the thermally suitable habitat patches became smaller and more exposed to the forest edge, resulting in 41.1% decline of metapopulation capacity, which indicated that giant pandas were facing high risks of habitat fragmentation. The protected thermally suitable habitat increased by 3.5 times from 1980 to 2010. In contrast to the habitat fragmentation outside nature reserves, the nature reserves have mitigated the habitat loss and fragmentation for giant pandas. Our findings suggested that conservation effort to reduce giant panda extinction risk should focus on maintaining spatial matching between panda and bamboo in the face of future climate change

SUMO1 modification of KHSRP regulates tumorigenesis by preventing the TL-G-Rich miRNA biogenesis

Abstract Background MicroRNAs (miRNAs) are important regulators involved in diverse physiological and pathological processes including cancer. SUMO (small ubiquitin-like modifier) is a reversible protein modifier. We recently found that SUMOylation of TARBP2 and DGCR8 is involved in the regulation of the miRNA pathway. KHSRP is a single stranded nucleic acid binding protein with roles in transcription and mRNA decay, and it is also a component of the Drosha-DGCR8 complex promoting the miRNA biogenesis. Methods The in vivo SUMOylation assay using the Ni2+-NTA affinity pulldown or immunoprecipitation (IP) and the in vitro E.coli-based SUMOylation assay were used to analyze SUMOylation of KHSRP. Nuclear/Cytosol fractionation assay and immunofluorescent staining were used to observe the localization of KHSRP. High-throughput miRNA sequencing, quantantive RT-PCR and RNA immunoprecipitation assay (RIP) were employed to determine the effects of KHSRP SUMO1 modification on the miRNA biogenesis. The soft-agar colony formation, migration, vasculogenic mimicry (VM) and three-dimensional (3D) cell culture assays were performed to detect the phenotypes of tumor cells in vitro, and the xenograft tumor model in mice was conducted to verify that SUMO1 modification of KHSRP regulated tumorigenesis in vivo. Results KHSRP is modified by SUMO1 at the major site K87, and this modification can be increased upon the microenvironmental hypoxia while reduced by the treatment with growth factors. SUMO1 modification of KHSRP inhibits its interaction with the pri-miRNA/Drosha-DGCR8 complex and probably increases its translocation from the nucleus to the cytoplasm. Consequently, SUMO1 modification of KHSRP impairs the processing step of pre-miRNAs from pri-miRNAs which especially harbor short G-rich stretches in their terminal loops (TL), resulting in the downregulation of a subset of TL-G-Rich miRNAs such as let-7 family and consequential tumorigenesis. Conclusions Our data demonstrate how the miRNA biogenesis pathway is connected to tumorigenesis and cancer progression through the reversible SUMO1 modification of KHSRP