Relation of study quality, concordance, take home message, funding, and impact in studies of influenza vaccines: systematic review

Objective To explore the relation between study concordance, take home message, funding, and dissemination of comparative studies assessing the effects of influenza vaccines

Dynamics of the weed community during pineapple growth in the Brazilian semi-arid region.

The pineapple belongs to the family Bromeliaceae and is a slow-growing succulent monocot with a reduced superficial root system. For this reason, the interference of weeds in competition with this crop can cause significant losses to the production. One of the bases to elaborate a control strategy is the knowledge of the diversity of weeds that occur in the cultivated areas. The objective of this study was to identify the weed community during pineapple growth in a semi-arid climate region of Brazil. Weeds were collected 60, 120, 180, 240, 300, and 360 days after planting (DAP) the pineapple. These collections were made in three different plots every two months until floral induction, composed of three pineapple cultivars. The weed community found in the irrigated pineapple field, in semi-arid climate conditions, was mostly composed by species belonging to the families Amaranthaceae, Asteraceae, Convolvulaceae, Fabaceae, Malvaceae, and Poaceae. The highest diversity of weed species was found at 60 DAP. The species Ipomoea acuminata was present throughout the development of the pineapple and showed the highest importance value index in most of the periods evaluated during the pineapple growth.Título em espanhol: Dinámica de la comunidad de malezas a lo largo del crecimiento de piña en la región semiárida brasileña

Type I interferon/IRF7 axis instigates chemotherapy-induced immunological dormancy in breast cancer

Neoadjuvant and adjuvant chemotherapies provide survival benefits to breast cancer patients, in particular in estrogen receptor negative (ER-) cancers, by reducing rates of recurrences. It is assumed that the benefits of (neo)adjuvant chemotherapy are due to the killing of disseminated, residual cancer cells, however, there is no formal evidence for it. Here, we provide experimental evidence that ER- breast cancer cells that survived high-dose Doxorubicin and Methotrexate based chemotherapies elicit a state of immunological dormancy. Hallmark of this dormant phenotype is the sustained activation of the IRF7/IFN-beta/IFNAR axis subsisting beyond chemotherapy treatment. Upregulation of IRF7 in treated cancer cells promoted resistance to chemotherapy, reduced cell growth and induced switching of the response from a myeloid derived suppressor cell-dominated immune response to a CD4(+)/CD8(+) T cell-dependent anti-tumor response. IRF7 silencing in tumor cells or systemic blocking of IFNAR reversed the state of dormancy, while spontaneous escape from dormancy was associated with loss of IFN-beta production. Presence of IFN-beta in the circulation of ER- breast cancer patients treated with neoadjuvant Epirubicin chemotherapy correlated with a significantly longer distant metastasis-free survival. These findings establish chemotherapy-induced immunological dormancy in ER- breast cancer as a novel concept for (neo)adjuvant chemotherapy activity, and implicate sustained activation of the IRF7/IFN-beta/IFNAR pathway in this effect. Further, IFN-beta emerges as a potential predictive biomarker and therapeutic molecule to improve outcome of ER- breast cancer patients treated with (neo)adjuvant chemotherapy.Peer reviewe