Microsatellite genotypes of wild-caught sawfish, including putative parhenogens

Individual sawfish plus their genotypes at 16 microsatellite loci. Locus names are at the top of each column, and each locus is represented by two columns. Numbers represent sizes of PCR products (i.e. alleles). The first seven individuals in the spreadsheet are the putative parthenogens (Saw144, Saw146, Saw147, Saw153, Saw181, Saw008, and Saw169)

Compound character attributes (<i>c</i>CA) for CITES-listed shark species.

<p>Position numbers (bold) are from the beginning of the COI gene.</p><p>Compound character attributes (<i>c</i>CA) for CITES-listed shark species.</p

Feldheim et al. lemon shark morphological and catch data Bimini, Bahamas 1995-2012

The first worksheet of the excel file is entitled "explanation of data." This sheet contains a key for abbreviations and explanations of each subsequent sheet

Species-specific primers designed along with their sequences and expected amplicon sizes for each species.

<p>Rlal: <i>Rhizoprionodon lalandei</i>; Rtay: <i>R. taylori</i>; Rolig: <i>R. oligolinx</i>; Rter: <i>R. terranovae</i>; Rlong: <i>R. longurio</i>; Rpor: <i>R. porosus</i>; Racut: <i>R. acutus</i>.</p

Sequence matching results in NCBI BLAST and BOLD of unknown processed fins and fin soup samples.

<p># = fin sample identifier, Type = processed fin (P) or soup (S), Loc = Collection location (HK = Hong Kong, USA = United States of America), BOLD = 100% identification at the lowest taxon possible (genus or species) in a Fish Barcode of Life Initiative (FISH-BOL) search, BLAST top hit = closest match in GenBank BLAST search (Coverage, Identity and UNQ (wheter or not the match was unique to that species refer to this search), I.D. = best identification based on the two searches.</p><p>Sequence matching results in NCBI BLAST and BOLD of unknown processed fins and fin soup samples.</p

Triplex scheme of ITS2 species-diagnostic primers.

<p>Representation of the shark nuclear 5.8S and 28S ribosomal RNA genes and ITS2 locus showing relative annealing sites and orientation of the shark universal ITS2 primers (Fish 5.8SF and 28SR indicated by gray irregular pentagons). The Brazilian sharpnose (<i>R. lalandei</i>) Rlal293F primer is an example of a species-specific primer used in this study and is shown as a dark gray irregular pentagon. Also represented are the positive control and species-specific amplicons expected to be produced using this combination of three primers when tested against the target species, <i>R.lalandei</i>, DNA (Figure adapted from Shivji et al. 2002).</p

Genetic distances within and between sharpnose sharks calculated as pairwise Tamura-Nei for the nuclear ITS2 locus.

<p>N/C: intra-specific genetic distances not calculated since only one animal sequenced.</p

Nonaplex scheme of ITS2 species-diagnostic primers.

<p>Representation of the shark nuclear 5.8S and 28S ribosomal RNA genes and ITS2 locus showing relative annealing sites and orientation of primers used in the nonaplex-PCR assay. Shark universal primers (Fish 5.8SF and Fish 28SR) are shown as gray irregular pentagons, while the seven sharpnose species-specific primers are shown by dark gray irregular pentagons. Rlal: <i>Rhizoprionodon lalandei</i>; Rtay: <i>R. taylori</i>; Rolig: <i>R. oligolinx</i>; Rter: <i>R. terranovae</i>; Rlong: <i>R. longurio</i>; Rpor: <i>R. porosus</i>; Racut: <i>R. acutus</i>.</p