Antennal detection of sex pheromone by female Pandemis limitata (Robinson) (Lepidoptera: Tortricidae) and its impact on their calling behaviour

Previous observations lead us to believe that female Pandemis limitata (Robinson) (0 to 24 h old) are as attractive as their pheromone gland extract to males in clean air, but are more attractive in an environment permeated with their major pheromone component(Z)-11-tetradecenyl acetate. Therefore, in this study, we tested the hypothesis that females can detect and/or respond to their pheromone components. Using electroantennographic detection, we found female P. limitata able to perceive both of their known pheromone components, (Z)-11-tetradecenyl acetate and (Z)-9-tetradecenyl acetate. Female antennal response was found to be 46.3% weaker than that of males, under identical conditions, with male antennae producing significantly higher deflections to the higher pheromone doses tested and to the plant volatile,(E)-2-hexanal. Observations of females in clean air versus (Z)-11-tetradecenyl acetate-permeated air showed no significant differences with respect to onset time, frequency or duration of calling. Females moved significantly less often in a (Z)-11-tetradecenyl acetate-permeated portion of a flight tunnel than in the corresponding clean-air portion

Bayou virus-associated hantavirus pulmonary syndrome in Eastern Texas: identification of the rice rat, Oryzomys palustris, as reservoir host.

We describe the third known case of hantavirus pulmonary syndrome (HPS) due to Bayou virus, from Jefferson County, Texas. By using molecular epidemiologic methods, we show that rice rats (Oryzomys palustris) are frequently infected with Bayou virus and that viral RNA sequences from HPS patients are similar to those from nearby rice rats. Bayou virus is associated with O. palustris; this rodent appears to be its predominant reservoir host

Romanticising the Past: Core Socialist Values and the China Dream as Legitimisation Strategy

This article examines “core socialist values” as a part of the China Dream discourse, in the context of the Chinese Communist Party (CCP)’s search for alternative sources of legitimacy. Using the “visualising our values” poster collection and the “China Dream Child” campaign as case studies, this article argues that such narratives form a crucial part of the CCP’s continuing legitimisation strategy, where the party emphasises its role in providing moral authority and guidance for the general public. In order to lay such claims, the narratives focus on romanticising and homogenising both the imperial and the socialist past, while projecting a strong sense of optimism for the future, based on similar hopes of continuity and homogeneity

An unlikely partnership: fishers’ participation in a small-scale fishery data collection program in the Timor Sea

Traditional fisheries stock assessment methods and fishery independent surveys are costly and time consuming exercises. However fishers trained in data collection and utilising other skills can reduce costs and improve fishery assessments and management. A data collection program was conducted by Australian and Indonesian scientists with small-scale Indonesian sea cucumber fishers to evaluate the approach and then capture its benefits. The data fishers recorded allowed for the first stock assessment of this trans-boundary fishery during its centuries-long existence at Scott Reef in north-western Australia. The program also included interviews with fishers capturing the social, economic, and demographic aspects of the fishery. Economic inputs to fishing were complemented by fishery revenue data voluntarily submitted when fishers returned to port and sold their catch. Catch data recorded by fishers demonstrated much higher abundances than estimates obtained using standard visual transect methods and accurately reflected the true catch composition. However, they also showed extreme rates of exploitation. Interviews revealed social and economic factors that would be important considerations if management interventions were made. The program’s approach and the time scientists spent on the fishers’ vessels were key ingredients to fishers’ participation and the utility of the results. Despite the program’s achievements the information generated has not led to improved management or had any direct benefits for the participants. Sustaining the program in the longer term requires that its value is better captured

E6-mediated activation of JNK drives EGFR signalling to promote proliferation and viral oncoprotein expression in cervical cancer

Human papillomaviruses (HPV) are a major cause of malignancy worldwide, contributing to ~5% of all human cancers including almost all cases of cervical cancer and a growing number of ano-genital and oral cancers. HPV-induced malignancy is primarily driven by the viral oncogenes, E6 and E7, which manipulate host cellular pathways to increase cell proliferation and enhance cell survival, ultimately predisposing infected cells to malignant transformation. Consequently, a more detailed understanding of viral-host interactions in HPV-associated disease offers the potential to identify novel therapeutic targets. Here, we identify that the c-Jun N-terminal kinase (JNK) signalling pathway is activated in cervical disease and in cervical cancer. The HPV E6 oncogene induces JNK1/2 phosphorylation in a manner that requires the E6 PDZ binding motif. We show that blockade of JNK1/2 signalling using small molecule inhibitors, or knockdown of the canonical JNK substrate c-Jun, reduces cell proliferation and induces apoptosis in cervical cancer cells. We further demonstrate that this phenotype is at least partially driven by JNK-dependent activation of EGFR signalling via increased expression of EGFR and the EGFR ligands EGF and HB-EGF. JNK/c-Jun signalling promoted the invasive potential of cervical cancer cells and was required for the expression of the epithelial to mesenchymal transition (EMT)-associated transcription factor Slug and the mesenchymal marker Vimentin. Furthermore, JNK/c-Jun signalling is required for the constitutive expression of HPV E6 and E7, which are essential for cervical cancer cell growth and survival. Together, these data demonstrate a positive feedback loop between the EGFR signalling pathway and HPV E6/E7 expression, identifying a regulatory mechanism in which HPV drives EGFR signalling to promote proliferation, survival and EMT. Thus, our study has identified a novel therapeutic target that may be beneficial for the treatment of cervical cancer

In-vitro efficacy of antibiotics released from calcium sulfate bone void filler beads

Fifteen different antibiotics were individually mixed with a commercially available calcium sulfate bone void filler beads. The antibiotics were: amikacin, ceftriaxone, cefuroxime, ciprofloxacin, clindamycin, colistamethate sodium, daptomycin, gentamicin, imipenem/cilastatin, meropenem, nafcillin, rifampicin, teicoplanin, tobramycin and vancomycin. The efficacy of specific released antibiotics were validated by zone of inhibition (ZOI) testing using a modified Kirby—Bauer disk diffusion method against common periprosthetic joint infection pathogens. With a subset of experiments (daptomycin, rifampin, vancomycin alone and rifampin and vancomycin in combination) we investigated how release varied over 15 days using a repeated ZOI assay. We also tested the ability of these beads to kill biofilms formed by Staphylococcus epidermidis 35984, a prolific biofilm former. The results suggest that certain antibiotics can be combined and released from calcium sulfate with retained antibacterial efficacy. The daptomycin and rifampin plus vancomycin beads showed antimicrobial efficacy for the full 15 days of testing and vancomycin in combination with rifampin prevented resistant mutants. In the biofilm killing assay all of the antibiotic combinations showed a significant reduction in biofilm bacteria after 24 hr. The exposure time was an important factor in the amount of killing and varied among the antibiotics

In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections

Introduction. Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation. DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement. Antibiotic loaded beads could inhibit the growth of polymicrobial DFI communities with differing compositions in vitro.Aim. This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology. Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates. Calcium sulfate beads containing a combination of vancomycin and gentamicin were then placed on the surface of the agar and following incubation, zones of inhibition (ZOI) were measured. For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results. Calcium sulfate beads loaded with a combination of vancomycin and gentamicin were able to inhibit bacterial growth from all polymicrobial tissue homogenates tested, with ZOI diameters ranging from 15 to 40 mm. In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion. The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests. These results show promise for clinical application, but further research and clinical studies are required.1 year embargo from time of publicationPublished version, accepted version, submitted versio