Exploring the Utility of High Resolution Imagery for Determining Wetland Signatures

Indiana University-Purdue University Indianapolis (IUPUI)Wetland habitats are characterized by periodic inundation and saturation by water creating anaerobic conditions that generate hydric soils and support hydrophytic vegetation. Wetland habitats provide important ecological functions including breeding grounds for fish, other wildlife, water purification, reduction in flooding, species diversity, recreation, food production, aesthetic value, and transformation of nutrients (Tiner, 1999). The multiple benefits of wetlands make them an important resource to monitor. A literature review suggests a combination of geospatial variables and methods should be tested for appropriateness in wetland delineation within local settings. Advancements in geospatial data technology and ease of accessing new, higher resolution geospatial data make study at local levels easier and more feasible (Barrette et al, 2000). The purpose of the current study is to evaluate new sources of geospatial data as potential variables to improve wetland identification and delineation. High resolution multispectral digital imagery, topographic data, and soils information are used to derive and evaluate independent variables. Regression analysis was used to analyze the data

Risk, innovation and BSE : cattle farmer perspectives on an agricultural and health emergency

This ethnographic research examines how farmers survive agricultural crises by exploring reactions of Saskatchewan beef and dairy farmers to the Canadian BSE crisis. As this study unfolded it became clear that the BSE crisis is only one of many recent crises that have been changing the face of Saskatchewan rural communities and family farms. Producers see a crisis in their inability to achieve their own measures of success in both the life and business of farming. This includes a greater need for off-farm work, a decline in rural community life and values, and a shift away from farming as a desirable livelihood. The BSE crisis has highlighted the risky nature of the contemporary agriculture industry, both for farmers' livelihoods and for food safety. Farmers' initial strategies to address the BSE crisis were precautionary and conservative in nature: minimal enterprise adaptation while waiting out markets. As the crisis continued, producers worked to bring their experience and understanding to bear on changing the structure of the agricultural system. Attempts at change were not often successful. This was attributed to a lack of initiative by government and powerful players, such as the multi-national packing industry that profited from the crisis situation and used the crisis to consolidate power within the value chain. Producers felt that they were paying too much for risks that were beyond their control. The government support they needed was not in line with their structural concerns; risky pre-BSE structures have not been appreciably changed. Uncertainty and risk remain high for the average farmer. There appears to be a growing distrust in powerful institutions that farmers depend on, and a consequent disengagement from government surveillance and regulatory policies. This foreshadows possible serious repercussions in food security and food safety, issues that are still unsettled regarding BSE in Canada. This research indicates a need for greater transparency and public knowledge pathways to reduce uncertainty and allow individuals to better understand and manage emerging risk complexes. Increased democratic space within food and agricultural systems for participation by producer and rural publics would help to balance out government rationalities that may not fully account for culturally mediated understandings of risk and action at the farm level

The role of the PSD95/Dlg/ZO-1 (PDZ) binding motif of human papillomavirus type 18 E6 oncoprotein in the virus life cycle

A PSD95/Dlg/ZO-1(PDZ)-binding motif (PBM) in the E6 protein of high-risk, cancer-causing human papillomaviruses (HPV) targets a subset of cellular PDZ domain-containing proteins involved in diverse regulatory processes including cell polarity and proliferation, for proteasome-mediated degradation. Interaction with this select group of PDZ domain-containing proteins is negatively regulated by cAMP-dependent protein kinase (PKA) mediated phosphorylation of the E6 PBM. This thesis has sought to address the hypothesis that the PBM of E6 plays an important role within the HPV life cycle. This study has shown that deletion of the E6 PBM from HPV18 genomes affects the morphology and growth of viral episome-containing human keratinocytes and furthermore links E6 PBM function to viral episome replication (maintenance replication and differentiation-dependent amplification). Loss of negative regulation of the E6 PBM by mutation of the PKA recognition motif was associated with increased cell growth and indeed the growth of wildtype HPV18 genome-containing cells responded to changes in PKA signalling. Constitutive E6 PBM function was also associated with invasion of cells suggesting that malignant progression of HPV-infected cells may be linked to changes in PKA signalling. Modulation of the E6 PBM function in the viral genome-containing cells was associated with a change in protein levels of the PDZ domain-containing protein discs large (hDlg) and changes in the non receptor protein phosphastase PTPN13 specific species

Grape leaf rust mite, Calepitrimerus vitis (Acari: Eriophyidae), a new pest of grapes in British Columbia

The grape leaf rust mite, Calepitrimerus vitis (Nalepa), was first discovered in the interior of British Columbia in 2009 on grape leaves from a commercial vineyard north of Osoyoos. Bronzing of grape leaves confirmed to be caused by C. vitis in summer 2009 was followed by severely stunted shoots and distorted leaves in several vineyards in spring 2010. Numbers and lengths of shoots and fruit clusters were reduced significantly on vines infested with C. vitis. Earlier studies have shown that outbreaks of C. vitis result from pesticide sprays targeted to other pests that damage predator mite populations. Sprays of sulphur-based fungicides early in the season are the recommended method of control

The E1 copper binding domain of full-length amyloid precursor protein mitigates copper-induced growth inhibition in brain metastatic prostate cancer DU145 cells

Copper plays an important role in the aetiology and growth of tumours and levels of the metal are increased in the serum and tumour tissue of patients affected by a range of cancers including prostate cancer (PCa). The molecular mechanisms that enable cancer cells to proliferate in the presence of elevated copper levels are, therefore, of key importance in our understanding of tumour growth progression. In the current study, we have examined the role played by the amyloid precursor protein (APP) in mitigating copper-induced growth inhibition of the PCa cell line, DU145. A range of APP molecular constructs were stably over-expressed in DU145 cells and their effects on cell proliferation in the presence of copper were monitored. Our results show that endogenous APP expression was induced by sub-toxic copper concentrations in DU145 cells and over-expression of the wild-type protein was able to mitigate copper-induced growth inhibition via a mechanism involving the cytosolic and E1 copper binding domains of the full-length protein. APP likely represents one of a range of copper binding proteins that PCa cells employ in order to ensure efficient proliferation despite elevated concentrations of the metal within the tumour microenvironment. Targeting the expression of such proteins may contribute to therapeutic strategies for the treatment of cancers

Stroma-induced Jagged1 expression drives PC3 prostate cancer cell migration:disparate effects of RIP-generated proteolytic fragments on cell behaviour and Notch signaling

The Notch ligand Jagged1 is subject to regulated intramembrane proteolysis (RIP) which yields a soluble ectodomain (sJag) and a soluble Jagged1 intracellular domain (JICD). The full-length Jagged1 protein enhances prostate cancer (PCa) cell proliferation and is highly expressed in metastatic cells. However, little is known regarding the mechanisms by which Jagged1 or its RIP-generated fragments might promote PCa bone metastasis. In the current study we show that bone marrow stroma (BMS) induces Jagged1 expression in bone metastatic prostate cancer PC3 cells and that this enhanced expression is mechanistically linked to the promotion of cell migration. We also show that RIP-generated Jagged1 fragments exert disparate effects on PC3 cell behaviour and Notch signaling. In conclusion, the expression of both the full-length ligand and its RIP-generated fragments must be considered in tandem when attempting to regulate Jagged1 as a possible PCa therapy

Antennal detection of sex pheromone by female Pandemis limitata (Robinson) (Lepidoptera: Tortricidae) and its impact on their calling behaviour

Previous observations lead us to believe that female Pandemis limitata (Robinson) (0 to 24 h old) are as attractive as their pheromone gland extract to males in clean air, but are more attractive in an environment permeated with their major pheromone component(Z)-11-tetradecenyl acetate. Therefore, in this study, we tested the hypothesis that females can detect and/or respond to their pheromone components. Using electroantennographic detection, we found female P. limitata able to perceive both of their known pheromone components, (Z)-11-tetradecenyl acetate and (Z)-9-tetradecenyl acetate. Female antennal response was found to be 46.3% weaker than that of males, under identical conditions, with male antennae producing significantly higher deflections to the higher pheromone doses tested and to the plant volatile,(E)-2-hexanal. Observations of females in clean air versus (Z)-11-tetradecenyl acetate-permeated air showed no significant differences with respect to onset time, frequency or duration of calling. Females moved significantly less often in a (Z)-11-tetradecenyl acetate-permeated portion of a flight tunnel than in the corresponding clean-air portion

Bayou virus-associated hantavirus pulmonary syndrome in Eastern Texas: identification of the rice rat, Oryzomys palustris, as reservoir host.

We describe the third known case of hantavirus pulmonary syndrome (HPS) due to Bayou virus, from Jefferson County, Texas. By using molecular epidemiologic methods, we show that rice rats (Oryzomys palustris) are frequently infected with Bayou virus and that viral RNA sequences from HPS patients are similar to those from nearby rice rats. Bayou virus is associated with O. palustris; this rodent appears to be its predominant reservoir host

Assessing the bioactive profile of anti-fungal loaded calcium sulfate against fungal biofilms

Calcium sulfate (CS) has been used clinically as a bone or void filling biomaterial, and due to its resorptive properties have provided the prospect for its use as a release mechanism for local antibiotics to control biofilms. Here, we aimed to test CS beads loaded with three antifungal drugs against planktonic and sessile fungal species to assess whether these antifungal beads could be harnessed to provide consistent release of antifungals at biofilm inhibitive doses. A panel of different fungal species (n=15) were selected for planktonic broth microdilution testing with fluconazole (FLZ), amphotericin B (AMB) and caspofungin (CSP). After establishing planktonic inhibition, antifungal CS beads were introduced to fungal biofilms (n=5) to assess biofilm formation and cell viability through a combination of standard quantitative and qualitative biofilm assays. Inoculation of a hydrogel substrate, packed with antifungal CS beads, was also used to assess diffusion through a semi-dry material, to mimic active infection in-vivo. In general, antifungals released from CS loaded beads were all effective at inhibiting the pathogenic fungi over 7-days within standard MIC ranges for these fungi. We observed a significant reduction of pre-grown fungal biofilms across key fungal pathogens following treatment, with visually observable changes in cell morphology and biofilm coverage provided by scanning electron microscopy. Assessment of biofilm inhibition also revealed reductions in total and viable cells across all organisms tested. These data show that antifungal loaded CS beads produce a sustained antimicrobial effect, which inhibits and kills clinically relevant fungal species in-vitro as planktonic and biofilm cells