Development of 55 novel polymorphic microsatellite loci for the critically endangered L. (Actinopterygii: Perciformes: Percidae) and cross-species amplification in five other percids

International audienceBy combining biotin-enrichment protocol and next generation pyrosequencing, through 454 GS-FLX Titanium technology, 55 polymorphic microsatellites loci with perfect motif were isolated from the Rhone streber (), a critically endangered European fish species. Eight multiplex PCR kits were optimised in order to genotype a total of 58 polymorphic loci, including three previously published loci. The level of genetic diversity was assessed for 68 , 30 , 33 and four individuals. Amplification success was also assessed on and using single individuals. These markers will be useful to investigate the population structure of the highly fragmented Rhone streber. They represent a powerful tool for conservation issues and evolutionary approaches of this endemic species. Moreover, part of our markers demonstrated applicability to other percid species, allowing for potential applications to fisheries and aquaculture management

Population differentiation in megrim (Lepidorhombus whiffiagonis) and four spotted megrim (Lepidorhombus boscii) across Atlantic and Mediterranean waters and implications for wild stock management

International audienc

Apports de l'écologie comportementale à la conservation d'un poisson en voie de disparition (l'apron du Rhône (Zingel Asper))

L'apron (Zingel Asper), poisson endémiquedu bassin du Rhône, est menacé de disparition. L'adaptation des modèles de capture-marquage-recapture (CMR) à l'étude de ses préférences de micro et de méso-habitat (faciès) ainsi que les travaux de génétique (relations d'apparentement, dispersion juvénile) ont révélé l'importance des déplacements, de l'hétérogénéité spatiale des habitats et du système d'appariement dans le maintien d'une des dernières populations viable d'apron (rivière Beaume, Ardèche). Le protocole de CMR a également permis l'estimation des rythmes de croissance et leur validation. Les résultats acquis au cours de ce travail ont été discutés en fonction des perspectives de conservation de l'espèceLYON1-BU.Sciences (692662101) / SudocSudocFranceF

Isolation and characterization of the first eight microsatellite loci in Gammarus fossarum (Crustacea,Amphipoda) and cross-amplification in G. pulex and G. orinos.

International audienceWe characterized the first microsatellite markers for Gammarus fossarum. Eight loci gave satisfactory amplification patterns in two stream populations (Southern France) with number of alleles ranging from 2 to 10 and expected heterozygosity from 0.076 to 0.857. We performed cross-amplification in two closely related gammarid species, Gammarus pulex and Gammarus orinos. Among the eight tested microsatellite loci, four correctly amplified in G. pulex and three in G. orinos

Capture-recapture estimates of space used in streams (CRESUS) at the population scale: case study on Zingel asper (percid), a threatened species of the Rhône catchment

International audienceIn this study, capture-mark-recapture statistics were applied to spatial recapture histories to assess the intensity of fish restricted movements along the longitudinal axis of a river using a previously described model for survival and recruitment analysis. Adapting the stopover estimation method to spatial data, movement probabilities were then used to estimate space used at the population scale. This capture-recapture estimates of space used in streams (CRESUS) method may thus be seen as a complementary tool of classic home range methods and should be used to explore the consequence of behavioural strategies on population mechanisms. We propose a methodological example where movements and space use strategies of a Zingel asper (percid) population in the Beaume River (Ardèche, France) were directly estimated at the population scale taking account of the effects of different biotic or abiotic factors. Results showed differences in Z. asper space use patterns among sexes, periods of biological cycle (growing and spawning period), and types of mesohabitat. Downstream movements were more important during the spawning period and by the way the riffle was more intensively used