Benefits and costs to pollinating, seed-eating insects: the effect of flower size and fruit abortion on larval performance

Plant-pollinator interactions are well-known examples of mutualism, but are not free of antagonism. Antagonistic interactions and defenses or counter-defenses are expected particularly in nursery pollination. In these systems, adult insects, while pollinating, lay their eggs in flowers, and juveniles consume the seeds from one or several fruits, thereby substantially reducing plant fitness. The outcome of such interactions will depend, for the plant, on the balance between pollination versus seed predation and for the larvae on the balance between the food and shelter provided versus the costs imposed by plant defenses, e.g., through abortion of infested fruits. Here, we examine the costs and benefits to the larvae in the nursery-pollination system Silene latifolia/Hadena bicruris. Using selection lines that varied in flower size (large- vs. small-flowered plants), we investigated the effects of variation in flower and fruit size and of a potential defense, fruit abortion, on larval performance. In this system, infested fruits are significantly more likely to be aborted than non-infested fruits; however, it is unclear whether fruit abortion is effective as a defense. Larger flowers gave rise to larger fruits with more seeds, and larvae that were heavier at emergence. Fruit abortion was frequently observed (ca. 40% of the infested fruits). From aborted fruits, larvae emerged earlier and were substantially lighter than larvae emerging from non-aborted fruits. The lower mass at emergence of larvae from aborted fruits indicates that abortion is a resistance mechanism. Assuming that lower larval mass implies fewer resources invested in the frugivore, these results also suggest that abortion is likely to benefit the plant as a defense mechanism, by limiting both resources invested in attacked fruits, as well as the risk of secondary attack. This suggests that selective fruit abortion may contribute to the stability of mutualism also in this non-obligate syste

Pinyon pine mortality alters communities of ground-dwelling arthropods

We documented the effect of drought-induced mortality of pinyon pine (Pinus edulis Engelm.) on communities of ground-dwelling arthropods. Tree mortality alters microhabitats utilized by ground-dwelling arthropods by increasing solar radiation, dead woody debris, and understory vegetation. Our major objectives were to determine (1) whether there were changes in community composition, species richness, and abundance of ground-dwelling arthropods associated with pinyon mortality and (2) whether specific habitat characteristics and microhabitats accounted for these changes. We predicted shifts in community composition and increases in arthropod diversity and abundance due to the presumed increased complexity of microhabitats from both standing dead and fallen dead trees. We found significant differences in arthropod community composition between high and low pinyon mortality environments, despite no differences in arthropod abundance or richness. Overall, 22% (51 taxa) of the arthropod community were identified as being indicators of either high or low mortality. Our study corroborates other research indicating that arthropods are responsive to even moderate disturbance events leading to changes in the environment. These arthropod responses can be explained in part due to the increase in woody debris and reduced canopy cover created by tree mortality

Ground-dwelling arthropod responses to succession in a pinyon-juniper woodland

Stand-replacing wildfire is an infrequent but important disturbance in southwestern pinyon-juniper woodlands. A typical successional cycle in these woodlands is approximately 300 years or more after a stand-replacing fire. Arthropods, especially ground-dwelling taxa, are one of the most abundant and diverse fauna in terrestrial ecosystems and are typically responsive to microhabitat change. Little is known regarding community responses of ground-dwelling arthropods to changes in woodland successional stages from early ecosystems dominated by grasses, herbaceous plants, and fire adapted shrubs to tree-dominated old-growth ecosystems. In 2007 and 2008, within Mesa Verde National Park, Colorado, we compared the community composition of ground-dwelling arthropods between old-growth pinyon-juniper stands that were 300–400 years old and early successional areas recovering from a stand-replacing fire in 2002. The 2002 fire eliminated the dominant woody vegetation, which was replaced by increased herbaceous vegetation and bare ground. The early successional arthropod community showed a significantly higher abundance in major arthropod taxonomic groups, except spiders, compared to old-growth woodland. Old-growth species richness was greater in late August–September, 2007 and greater in early successional habitats during April–July, 2008. Spatial variability of the habitat was much greater in the recently burned early successional plots than the old-growth late successional plots. The differences in habitat were strongly correlated with arthropod community composition, suggesting that ground-dwelling arthropods are very sensitive to habitat changes. Habitat affiliation was strong, with 83% (early succession ruderal) and 91% (old-growth woodland) of the species found primarily or exclusively in one habitat. Many habitat indicator species (defined as species found in significantly greater abundance in one habitat) were found in both burned and old-growth habitats. Several species were found to be strict specialists exclusive to only one of these habitats. Collectively, the results suggest that heightened concern over loss of old-growth woodlands is warranted, given the distinct nature of the ground-dwelling arthropod community in old-growth habitats

Seismic imaging of the metamorphism of young sediment into new crystalline crust in the actively rifting Imperial Valley, California

Plate-boundary rifting between transform faults is opening the Imperial Valley of southern California and the rift is rapidly filling with sediment from the Colorado River. Three 65–90 km long seismic refraction profiles across and along the valley, acquired as part of the 2011 Salton Seismic Imaging Project, were analyzed to constrain upper crustal structure and the transition from sediment to underlying crystalline rock. Both first arrival travel-time tomography and frequency-domain full-waveform inversion were applied to provide P-wave velocity models down to ∼7 km depth. The valley margins are fault-bounded, beyond which thinner sediment has been deposited on preexisting crystalline rocks. Within the central basin, seismic velocity increases continuously from ∼1.8 km/s sediment at the surface to >6 km/s crystalline rock with no sharp discontinuity. Borehole data show young sediment is progressively metamorphosed into crystalline rock. The seismic velocity gradient with depth decreases approximately at the 4 km/s contour, which coincides with changes in the porosity and density gradient in borehole core samples. This change occurs at ∼3 km depth in most of the valley, but at only ∼1.5 km depth in the Salton Sea geothermal field. We interpret progressive metamorphism caused by high heat flow to be creating new crystalline crust throughout the valley at a rate comparable to the ≥2 km/Myr sedimentation rate. The newly formed crystalline crust extends to at least 7–8 km depth, and it is shallower and faster where heat flow is higher. Most of the active seismicity occurs within this new crust

George Whitefield's Preaching: An Evangelical Response to the Enlightenment

This dissertation examines the preaching ministry of George Whitefield as a precursor to presuppositional apologetics in relation to the cultural shift of the Enlightenment. The presupposition of the authority of the Word of God is the concept that bridges Whitefield’s preaching and presuppositional apologetics. Chapter 1 presents the research questions narrowing the topic. This section also presents background information as well as methodology of this work. Chapter 2 describes the biographical information of Whitefield. The cultural influences marked his life from an early age, but it was the influence of John and Charles Wesley that guided his faith. Chapter 3 outlines the thought of the Enlightenment period. Specifically, rationalism as a means to discerning and living by truth. Philosophical influences such as Locke, Newton, Hume, Descartes, and Kant are overviewed. The First Great Awakening was the result of many cultural and theological ideas boiling over simultaneously. People were craving truth, direction, and a sense of authority. The Awakening highlighted the movement of the Holy Spirit theologically and a resurgence of biblical truth culturally. The work of John Wesley and Jonathan Edwards is also detailed in regards to their efforts to combat rationalism as sole priority in worldview thinking. Chapter 4 highlights the apologetic within the polemic of Whitefield. His Calvinistic theology is identified and connected to the Articles of faith for the Church of England. This leads to the idea of presuppositional apologetics found within his preaching. Cornelius Van Til, the father of presuppositional apologetics, is also overviewed to show the distinct connection between Whitefield’s thoughts in holding a high view of Scripture and what becomes presuppositional apologetics. Chapter 5 examines the sermons of Whitefield for specific content related to evangelical pushback against Enlightenment thought. Key propositions of the Enlightenment regarding authority, morality, and ultimate truth are answered within the preaching of Whitefield. Each are examined thoroughly. Chapter 6 presents a conclusion regarding the apologetic of Whitefield in his preaching towards the Enlightenment. It reveals connections between the presupposition of the authority of the revelation of God and his polemic. Other connections between the reformer John Calvin and the father of presuppositional apologetics, Cornelius Van Til, are also noted

Postcopulatory sexual selection

The female reproductive tract is where competition between the sperm of different males takes place, aided and abetted by the female herself. Intense postcopulatory sexual selection fosters inter-sexual conflict and drives rapid evolutionary change to generate a startling diversity of morphological, behavioural and physiological adaptations. We identify three main issues that should be resolved to advance our understanding of postcopulatory sexual selection. We need to determine the genetic basis of different male fertility traits and female traits that mediate sperm selection; identify the genes or genomic regions that control these traits; and establish the coevolutionary trajectory of sexes

On the importance of balancing selection in plants

This is the peer reviewed version of the following article: Delph, L. F. and Kelly, J. K. (2014), On the importance of balancing selection in plants. New Phytol, 201: 45–56. doi:10.1111/nph.12441, which has been published in final form at http://doi.org/10.1111/nph.12441. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.Balancing selection refers to a variety of selective regimes that maintain advantageous genetic diversity within populations. We review the history of the ideas regarding the types of selection that maintain such polymorphism in flowering plants, notably heterozygote advantage, negative frequency-dependent selection, and spatial heterogeneity. One shared feature of these mechanisms is that whether an allele is beneficial or detrimental is conditional on its frequency in the population. We highlight examples of balancing selection on a variety of discrete traits. These include the well-referenced case of self-incompatibility and recent evidence from species with nuclear-cytoplasmic gynodioecy, both of which exhibit trans-specific polymorphism, a hallmark of balancing selection. We also discuss and give examples of how spatial heterogeneity in particular, which is often thought unlikely to allow protected polymorphism, can maintain genetic variation in plants (which are rooted in place) as a result of microhabitat selection. Lastly, we discuss limitations of the protected polymorphism concept for quantitative traits, where selection can inflate the genetic variance without maintaining specific alleles indefinitely. We conclude that while discrete-morph variation provides the most unambiguous cases of protected polymorphism, they represent only a fraction of the balancing selection at work in plants