Biotechnological Production of Oligosaccharides — Applications in the Food Industry

Oligosaccharides are carbohydrates, composed of up to twenty monosaccharides linked by glycosydic bonds, widely used in food and pharmaceutical industries. These compounds can be obtained by extraction from natural sources (milk, vegetables, fruits), and by chemical or biotechnological processes. In the last case, chemical structures and composition of the generated oligosaccharides depend on the type and source of enzymes, and on process conditions, including the initial concentration of substrate. Among the various functions of nondigestible oligosaccharides, one that has attracted attention is its prebiotic potential. The intestinal benefits of prebiotics, such as fructooligosaccharides and inulin as well as their symbiotic association with probiotic bacteria, encompass prevention and treatment of infectious diseases, including viral or bacterial diarrhea, and chronic inflammatory diseases such as ulcerative colitis. Other benefits attributed to prebiotics and probiotics include treatment of inflammatory intestinal and irritable bowel syndrome, prevention of cancer, and modulation of the immune system, mineral absorption and lipid metabolism. Fructooligosaccharides (FOS), galactooligosaccharides (GOS) and chitooligosaccharides (COS) have been widely studied for their prebiotic properties. Moreover, novel oligosaccharides with potential prebiotic activity are currently under investigation. This review will focus mainly on the biotechnological production, health benefits and applications of non-natural oligosaccharides in the food industry

Carotenoids in Raw Plant Materials

Carotenoids are rich sources of pro-vitamin A. These compounds are usually obtained from pumpkins (Cucurbita maxima, C. pepo, and C. moschata), as well as orange and yellow sweet potatoes. Carotenoids are C40 tetraterpenoids, which stand out for their antioxidant activity. Among them are carotenes (very apolar carbon and hydrogen molecules, like lycopene, β-carotene, α-carotene) and oxygenated derivatives and xanthophylls composed of oxygenated functions (less apolar molecules such as lutein, zeaxanthin, cryptoxanthin). β-Carotene is the most commonly found carotenoid, accounting for 25–30% of the total carotenoid content of plants. It is also the most active carotenoid, with the highest bioconversibility in the human body. β-Carotene is a suppressor of tumorigenesis in the skin, lung, liver, and colon, promoting the cessation of the cell multiplication cycle. Thermal processing can affect the sensory characteristics and the antioxidant compounds, altering the antioxidant potential of foods. Time, temperature, and style of cooking are determinant conditions for the increase or decrease of total antioxidant activity. The biological activity of carotenoids depends on their bioaccessibility and solubilization in the gastrointestinal tract. The purpose of this chapter is to offer information about some raw plant materials containing carotenoids

Carotenoids in Yellow Sweet Potatoes, Pumpkins and Yellow Sweet Cassava

Carotenoids are the most widespread pigments in nature, extremely important for human health, but are highly unstable molecules especially when exposed to light, oxygen and heat. Many authors report the carotenoid\u27s importance, mainly its pro‐vitamin A (α‐ and β‐carotene) and, additionally, the antioxidant capacity of some of them. Currently, more than 600 carotenoids are known and characterized by their chemical structures. In vegetables, common pro‐vitamin A carotenoids include β‐carotene and its 9, 13 and 15 isomers, α‐carotene and β‐cryptoxanthin. Other common carotenoids such as lycopene, lutein and zeaxanthin do not have pro‐vitamin A activity but serve as natural antioxidants. They are found in many fruits and vegetables such as carrots, yellow sweet potatoes, yellow sweet cassava and pumpkins. Normally, in these plant materials, the β‐carotene is the most abundant. It is still used as natural food coloring, which is not very expensive, since enough 3–5 g of β‐carotene is used to impart a yellow color characteristic of a ton of margarine. There is also a description of its importance in the formation of compounds responsible for flavors that are of interest fragrance and food industries. The purpose of this chapter is to report the presence of pro‐vitamin A carotenoids, mainly the β‐carotene in pumpkins, yellow sweet potato and yellow sweet and bitter cassava

Effects of cooking methods on the iron and zinc contents in cowpea (Vigna unguiculata) to combat nutritional deficiencies in Brazil.

This study aimed to evaluate the effect of cooking methods by measuring the iron and zinc contents in cowpea cultivars before and after soaking to determine the retention of these minerals

Novel ultraviolet absorbers derived from cashew nut shell liquid: spectrophotometric, in silico and in vitro assays

The cashew nut shell liquid (CNSL) constituents were isolated by our group leading to four mixtures and seventeen pure compounds, which had chromophoric groups similar to organic ultraviolet (UV) absorbers. In addition, C15 and C8 CNSL-derivatives molecules were rationally planned as UV absorbers. Mixtures and isolated CNSL compounds were demonstrated to be non-phototoxic when evaluated in a phototoxicity assay using the yeast Saccharomyces cerevisiae. Considering the absorption values on the UV range, 6 compounds showed appropriate SPF values regarding the spectrophotometric test. Additionally, in silico and in vitro evaluations were performed, showing non-oral bioavailability, as well as non-mutagenic, non-genotoxic and non-phototoxic properties for the tested compounds. These results contribute favorably to the aimed use of the compounds under analysis as novel organic UV absorbers that have as precursor the phenolic lipid component of CNSL, a waste product obtained as the by-product of cashew nut food processing

Uso de novos compostos capazes de absorver radiação ultravioleta, composições contendo os mesmos e processos para sua preparação

Em 11/04/2006: Publicação do Pedido Arquivado Definitivamente - Art. 216 § 2º e Art. 17 § 2º da LPI. Publicação de pedido definitivamente arquivado devido a não apresentação de procuração ou devido à apresentação de um pedido posterior. Encerrada a instância administrativa.Não concedidaA presente invenção trata de novas moléculas capazes de absorver radiação ultravioleta obtidas a partir de modificações no líquido da castanha de caju; são também descritas composições destinadas à proteção de superfícies e processos químicos para a produção das referidas moléculas