369 research outputs found
Ferromagnetic 0-pi Junctions as Classical Spins
The ground state of highly damped PdNi based 0-pi ferromagnetic Josephson
junctions shows a spontaneous half quantum vortex, sustained by a supercurrent
of undetermined sign. This supercurrent flows in the electrode of a Josephson
junction used as a detector and produces a phi(0)/4 shift in its magnetic
diffraction pattern. We have measured the statistics of the positive or
negative sign shift occurring at the superconducting transition of such a
junction. The randomness of the shift sign, the reproducibility of its
magnitude and the possibility of achieving exact flux compensation upon field
cooling: all these features show that 0-pi junctions behave as classical spins,
just as magnetic nanoparticles with uniaxial anisotropy.Comment: 4 pages, 4 figure
Pharmacokinetics and antinociceptive effects of tramadol and its metabolite O-desmethyltramadol following intravenous administration in sheep
Although sheep are widely used as an experimental model for various surgical procedures there is a paucity of data on the pharmacokinetics and efficacy of analgesic drugs in this species. The aims of this study were to investigate the pharmacokinetics of intravenously (IV) administered tramadol and its active metabolite O-desmethyltramadol (M1) and to assess the mechanical antinociceptive effects in sheep. In a prospective, randomized, blinded study, six healthy adult sheep were given 4 and 6\u2009mg/kg tramadol and saline IV in a cross-over design with a 2-week wash-out period. At predetermined time points blood samples were collected and physiological parameters and mechanical nociceptive threshold (MNT) values were recorded. The analytical determination of tramadol and M1 was performed using high performance liquid chromatography. Pharmacokinetic parameters fitted a two- and a non-compartmental model for tramadol and M1, respectively. Normally distributed data were analysed by a repeated mixed linear model. Plasma concentration vs. time profiles of tramadol and M1 were similar after the two doses. Tramadol and M1 plasma levels decreased rapidly in the systemic circulation, with both undetectable after 6\u2009h following drug administration. Physiological parameters did not differ between groups; MNT values were not statistically significant between groups at any time point. It was concluded that although tramadol and M1 concentrations in plasma were above the human minimum analgesic concentration after both treatments, no mechanical antinociceptive effects of tramadol were reported. Further studies are warranted to assess the analgesic efficacy of tramadol in sheep
Early histologic findings of pulmonary SARS-CoV-2 infection detected in a surgical specimen
Despite the current pandemic season, reports on pathologic features of coronavirus disease 19 (Covid-19) are exceedingly rare at the present time. Here we describe the pathologic features of early lung involvement by Covid-19 in a surgical sample resected for carcinoma from a patient who developed SARS-CoV-2 infection soon after surgery. The main histologic findings observed were pneumocyte damage, alveolar hemorrhages with clustering of macrophages, prominent and diffuse neutrophilic margination within septal vessels, and interstitial inflammatory infiltrates, mainly represented by CD8+ T lymphocytes. These features are similar to those previously described in SARS-CoV-2 infection. Subtle histologic changes suggestive pulmonary involvement by Covid-19 may be accidentally encountered in routine pathology practice, especially when extensive sampling is performed for histology. These findings should be carefully interpreted in light of the clinical context of the patient and could prompt a pharyngeal swab PCR test to rule out the possibility of SARS-CoV-2 infection in asymptomatic patients
Glioblastoma cusa fluid protein profiling: A comparative investigation of the core and peripheral tumor zones
The present investigation aimed to characterize the protein profile of cavitating ultrasound aspirator fluid of newly diagnosed and recurrent glioblastoma comparing diverse zones of collection, i.e., tumor core and tumor periphery, with the aid of 5\u2010aminolevulinic acid fluorescence. The samples were pooled and analyzed in triplicate by LC\u2010MS following the shotgun proteomic approach. The identified proteins were then grouped to disclose elements exclusive and common to the tumor state or tumor zones and submitted to gene ontology classification and pathway overrepresentation analysis. The proteins common to the distinct zones were further investigated by relative quantitation, following a label free approach, to disclose possible differences of expression. Nine proteins, i.e., tubulin 2B chain, CD59, far upstream element\u2010binding, CD44, histone H1.4, caldesmon, osteopontin, tropomyosin chain and metallothionein\u20102, marked the core of newly diagnosed glioblastoma with respect to tumor periphery. Considering the tumor zone, including the core and the fluorescence positive periphery, the serine glycine biosynthesis, pentose phosphate, 5\u2010 hydroxytryptamine degredation, de novo purine biosynthesis and huntington disease pathways resulted statistically significantly overrepresented with respect to the human genome of reference. The fluorescence negative zone shared several protein elements with the tumor zone, possibly indicating the presence of pathological aspects of glioblastoma rather than of normal brain parenchyma. On the other hand, its exclusive protein elements were considered to represent the healthy zone and, accordingly, exhibiting no pathways overrepresentation. On the contrary to newly diagnosed glioblastoma, pathway overrepresentation was recognized only in the healthy zone of recurrent glioblastoma. The TGF\u3b2 signaling pathway, exclusively classified in the fluorescence negative periphery in newly diagnosed glioblastoma, was instead the exclusive pathway classified in the tumor core of recurrent glioblastoma. These results, preliminary obtained on sample pools, demonstrated the potential of cavitron ultrasonic sur gical aspirate fluid for proteomic profiling of glioblastoma able to distinguish molecular features specific of the diverse tumor zones and tumor states, possibly contributing to the understanding of the highly infiltrative capability and recurrent rate of this aggressive brain tumor and opening to potential clinical applications to be further investigated
Mediation of adenylyl cyclase sensitization by PTX-insensitive Gα oA , Gα i1 , Gα i2 or Gα i3
Chronic activation of mu-opioid receptors, which couple to pertussis toxin-sensitive Gα i/o proteins to inhibit adenylyl cyclase (AC), leads to a compensatory sensitization of AC. Pertussis toxin-insensitive mutations of Gα i/o subtypes, in which the pertussis toxin-sensitive cysteine is mutated to isoleucine (G ), were used to determine whether each of the Gα i/o subtypes is able to mediate sensitization of AC. G , G , G or G were individually transiently transfected into C6 glioma cells stably expressing the mu-opioid receptor, or transiently co-expressed with the mu-opioid receptor into human embryonic kidney (HEK)293T cells. Cells were treated with pertussis toxin to uncouple endogenous Gα i/o proteins, followed by acute or chronic treatment with the mu-opioid agonist, [D-Ala 2 ,N-Me-Phe 4 ,Gly 5 -ol]enkephalin (DAMGO). Each Gα i/o subtype mediated acute DAMGO inhibition of AC and DAMGO-induced sensitization of AC. The potency for DAMGO to stimulate sensitization was independent of the Gα i/o subtype, but the level of sensitization was increased in clones expressing higher levels of Gα i/o subunits. Sensitization of AC mediated by a component of fetal bovine serum, which was also dependent on the level of functional Gα i/o subunits in the cell, was observed. This serum-mediated sensitization partially masked mu-opioid-mediated sensitization when expressed as percentage overshoot due to an apparent increase in AC activity.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65707/1/j.1471-4159.2006.04176.x.pd
Viscous-Inviscid Interactions in a Boundary-Layer Flow Induced by a Vortex Array
In this paper we investigate the asymptotic validity of boundary layer
theory. For a flow induced by a periodic row of point-vortices, we compare
Prandtl's solution to Navier-Stokes solutions at different numbers. We
show how Prandtl's solution develops a finite time separation singularity. On
the other hand Navier-Stokes solution is characterized by the presence of two
kinds of viscous-inviscid interactions between the boundary layer and the outer
flow. These interactions can be detected by the analysis of the enstrophy and
of the pressure gradient on the wall. Moreover we apply the complex singularity
tracking method to Prandtl and Navier-Stokes solutions and analyze the previous
interactions from a different perspective
Revealing the electronic structure of a carbon nanotube carrying a supercurrent
Carbon nanotubes (CNTs) are not intrinsically superconducting but they can
carry a supercurrent when connected to superconducting electrodes. This
supercurrent is mainly transmitted by discrete entangled electron-hole states
confined to the nanotube, called Andreev Bound States (ABS). These states are a
key concept in mesoscopic superconductivity as they provide a universal
description of Josephson-like effects in quantum-coherent nanostructures (e.g.
molecules, nanowires, magnetic or normal metallic layers) connected to
superconducting leads. We report here the first tunneling spectroscopy of
individually resolved ABS, in a nanotube-superconductor device. Analyzing the
evolution of the ABS spectrum with a gate voltage, we show that the ABS arise
from the discrete electronic levels of the molecule and that they reveal
detailed information about the energies of these levels, their relative spin
orientation and the coupling to the leads. Such measurements hence constitute a
powerful new spectroscopic technique capable of elucidating the electronic
structure of CNT-based devices, including those with well-coupled leads. This
is relevant for conventional applications (e.g. superconducting or normal
transistors, SQUIDs) and quantum information processing (e.g. entangled
electron pairs generation, ABS-based qubits). Finally, our device is a new type
of dc-measurable SQUID
Imaging spontaneous currents in superconducting arrays of pi-junctions
Superconductors separated by a thin tunneling barrier exhibit the Josephson
effect that allows charge transport at zero voltage, typically with no phase
shift between the superconductors in the lowest energy state. Recently,
Josephson junctions with ground state phase shifts of pi proposed by theory
three decades ago have been demonstrated. In superconducting loops,
pi-junctions cause spontaneous circulation of persistent currents in zero
magnetic field, analogous to spin-1/2 systems. Here we image the spontaneous
zero-field currents in superconducting networks of temperature-controlled
pi-junctions with weakly ferromagnetic barriers using a scanning SQUID
microscope. We find an onset of spontaneous supercurrents at the 0-pi
transition temperature of the junctions Tpi = 3 K. We image the currents in
non-uniformly frustrated arrays consisting of cells with even and odd numbers
of pi-junctions. Such arrays are attractive model systems for studying the
exotic phases of the 2D XY-model and achieving scalable adiabatic quantum
computers.Comment: Pre-referee version. Accepted to Nature Physic
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