Effect of hesperidin dietary supplementation on growth performance, carcass traits and meat quality of rabbits

[EN] An experiment was conducted to examine the dose effects of hesperidin dietary supplementation on fattening rabbits’ growth performance, as well as carcass and meat quality characteristics. Forty-eight Hyla hybrid male weaned (35 d old) rabbits were purchased and randomly assigned to 3 dietary groups of 16 rabbits each and fed diets supplemented with the antioxidant hesperidin at 0, 1 and 2 g/kg feed. At 80 d of age, the rabbits were slaughtered and samples of Longissimus lumborum (LL) muscle were used to estimate meat quality traits. No significant differences were observed in body weight at the age of 80 d, feed conversion rate (35 to 80 d), or organ weights among the 3 groups. The pH, colour, percentage of released water, shear force values and intramuscular fat content of LL muscle were not significantly influenced by the dietary treatment. Hesperidin dietary supplementation at both levels reduced the polyunsaturated fatty acids (PUFAs), mainly arachidonic (C20:4n-6), docosapentaenoic (C22:5n-3) and eicosapentaenoic (C20:5n-3) (only at 2 g/kg), and PUFA/SFA ratio (P<0.01). Based on the malondialdehyde (MDA) values, hesperidin inclusion did not influence meat antioxidant status during the 9-d refrigerated storage at 4°C. Thus, we may conclude that dietary supplementation with hesperidin at the selected concentration levels did not generally influence growth performance, carcass traits, meat quality or antioxidant capacity in fattening rabbits, although meat values for PUFAs appeared to be decreased.Simitzis, P.; Babaliaris, C.; Charismiadou, M.; Papadomichelakis, G.; Goliomytis, M.; Symeon, G.; Deligeorgis, S. (2014). Effect of hesperidin dietary supplementation on growth performance, carcass traits and meat quality of rabbits. World Rabbit Science. 22(2):113-121. doi:10.4995/wrs.2014.1760.SWORD113121222Alasnier, C., & Gandemer, G. (1998). Fatty acid and aldehyde composition of individual phospholipid classes of rabbit skeletal muscles is related to the metabolic type of the fibre. Meat Science, 48(3-4), 225-235. doi:10.1016/s0309-1740(97)00096-xCastellini, C., Dal Bosco, A., Bernardini, M., & Cyril, H. . (1998). Effect of Dietary Vitamin E on the Oxidative Stability of Raw and Cooked Rabbit Meat. Meat Science, 50(2), 153-161. doi:10.1016/s0309-1740(98)00026-6Dalle Zotte, A., & Szendrő, Z. (2011). The role of rabbit meat as functional food. Meat Science, 88(3), 319-331. doi:10.1016/j.meatsci.2011.02.017De Blas, C., Mateos, G.G., 1998. Feed formulation. In: de Blas, C., Wiseman, J. (Eds.), The Nutrition of the Rabbit. Wallingford, UK. CAB International, 241-253.Fundacion Espa-ola para el Desarrollo de la Nutricion Animal (FEDNA), 2003. In: de Blas, C., Mateos, G.G., Rebollar, P.G. (Eds.), Tablas FEDNA de composicion y valor nutritivo de alimentos para la fabricacion de piensos compuestos. 2nd ed. FEDNA, Madrid, Spain.Folch J., Lees M., Stanley S.G.H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem., 226: 497-509.Sas Institute INC. 2005. Statistical analysis systems user's guide. Version 9.1.3. SAS Institute, Inc., Cary, NC.Wenk C. 2003. Herbs and botanicals as feed additives in monogastric animals. Asian-Austr. J. Anim. Sci., 16: 282-289

NMR metabolomics investigates the influence of flavonoid-enriched rations on chicken plasma

The use of flavonoids as dietary supplements is well established, mainly due to their intense antioxidant and anti-inflammatory properties. In the present study, hesperidin, naringin, and vitamin E were used as additives at different concentrations in poultry rations in order to achieve meat of improved quality. NMR metabolomics was applied to chicken blood serum samples to discern whether and how the enriched rations affected the animals&apos; metabolic profile. Variations in the metabolic patterns according to sustenance consumption were traced by orthogonal projections to latent structures discriminant analysis (OPLS-DA) models and were attributed to specific metabolites by using S-line plots. In particular, serum samples from chickens fed with vitamin E displayed higher concentrations of glycine and succinic acid compared to control samples, which were mainly characterized by betaine, formic acid, and lipoproteins. Samples from chickens fed with hesperidin were characterized by increased levels of lactic acid, citric acid, creatine, carnosine, creatinine, phosphocreatine, anserine, glucose, and alanine compared to control samples. Lastly, naringin samples exhibited increased levels of citric and acetic acids. Results verify the scalability of NMR metabolomics to highlight metabolite variations among chicken serum samples in relation to food rations

UHPLC–HRMS-based tissue untargeted metabolomics study of naringin and hesperidin after dietary supplementation in chickens

To date numerous metabolomic studies have been performed in order to characterize nutritional intervention studies. The aim of the current study was to present a comprehensive pipeline for characterizing the metabolic changes that occur in chickens tissues in response to naringin and hesperidin dietary supplementation. Forty-nine chickens were randomly divided into 3 groups: the first one fed with diet supplemented with naringin, the second with hesperidin whereas the control group was fed by commercial basal diet. After 30 days of administration chicken muscle samples were analyzed by UHPLC–HRMS whereas data were analyzed by the proposed pipeline. Three significant variables were detected to discriminate the control from the group after naringin administration and thirteen variables after hesperidin supplementation. Furthermore, a more detailed pipeline (encompassing multiple internal standards, internal validation of the clustering, extended statistical significance scores and multiple identification procedures) has been proposed aiming towards a more accurate untargeted analysis. © 201

Development and validation of a UPLC–ESI(-)–MS/MS methodology for the simultaneous quantification of hesperidin, naringin, and their aglycones in chicken tissue samples

Background: The dietary supplementation of livestock with antioxidants to improve the meat quality represents an active research area of high commercial impact. In order to investigate the optimal dosing, analytical methodologies need to be developed in various tissues to evaluate which concentration does remain in the tissue. Objective: We aimed to develop and validate a sensitive and specific methodology for the simultaneous quantitative determination of hesperidin, naringin, hesperetin, and naringenin in chicken tissue samples employing ultra-performance LC–tandem MS. Methods: Lipid extraction using cold chloroform was performed followed by protein precipitation by cold acetone. Chromatography was performed on a C18 column using a ternary gradient of water, acetonitrile, and isopropanol–acetonitrile–acetone (58+40+2, v/v) as the mobile phase. Detection was performed by electrospray ionization in negative ion mode with the selected reaction monitoring technique. Results: Calibration plots exhibited good linearity (r2 &amp;gt; 0.99) over the concentration range from 0.125 to 25 μg/g tissue for the four analytes, and the lower LOQ for the four analytes was 0.125 μg/g tissue. The repeatability as percent relative SD and precision as percent accuracy were &amp;lt;20 and &amp;gt;80%, respectively. Conclusions: The developed methodology was applied for the quantitative determination of hesperidin, naringin, hesperetin, and naringenin in tissue samples after dietary supplementation with 1.5 g/kg hesperidin and 1.5 g/kg naringin in Ross 308 broiler chickens. Highlights: This is the first methodology to access naringin, naringenin, hesperidin, and hesperetin in chicken tissue. It involved simple sample preparation, and the mass spectrometry based detection ensures high specificity and sensitivity. © 2020 AOAC International. All rights reserved

Development of a Validated UHPLC-ESI (-)-HRMS Methodology for the Simultaneous Quantitative Determination of Hesperidin, Hesperetin, Naringin, and Naringenin in Chicken Plasma

A highly sensitive and specific methodology has been developed for the simultaneous determination of the flavonoids hesperidin, hesperetin, naringin, and naringenin in chicken plasma employing UHPLC-HRMS (Orbitrap Velos). Plasma samples were preprocessed by protein precipitation with cold acetone. Analysis was carried out on an INTERCHIM UHPLC C18 column using aq. 0.1% glacial acetic acid, acetonitrile, and isopropanol/acetonitrile/acetone (58/40/2, v/v) as the mobile phase. Detection was performed by means of electrospray ionization (ESI) in the negative ion. All calibration curves exhibited good linearity (r 2 &amp;gt; 0.990) over the concentration range of 0.005 to 1 μg/mL with a lower limit of quantification (LLOQ) of 0.005 μg/mL for the four analytes. The repeatability and precision were within %RSD &amp;lt; 20 and accuracy within %Error &amp;lt; 20. No matrix effect or carry over was observed for the proposed methodology. Furthermore, as the quantitation procedures employing an Orbitrap analyzer are yet not well established, the impact of various parameters of MS/MS-based quantitation has been examined in order to achieve analytically solid results. The methodology was applied in plasma samples after dietary supplementation with 0.75 g/kg of feed (low-dose treatment) and 1.5 g/kg of feed (high-dose treatment) of hesperidin and naringin in Ross 308 broiler chickens. © 2019, Springer Science+Business Media, LLC, part of Springer Nature