Hydrogen sulfide activates TRPA1 and releases 5-HT from epithelioid cells of the chicken thoracic aorta

Epithelioid cells in the chicken thoracic aorta are chemoreceptor cells that release 5-HT in response to hypoxia. It is likely that these cells play a role in chemoreception similar to that of glomus cells in the carotid bodies of mammals. Recently, H2S was reported to be a key mediator of carotid glomus cell responses to hypoxia. The aim of the present study was to reveal the mechanism of action of H2S on 5-HT outflow from chemoreceptor cells in the chicken thoracic aorta. The 5-HT outflow induced by NaHS, an H2S donor, and Na2S3, a polysulfide, was measured by using a HPLC equipped with an electrochemical detector. NaHS (0.3-3 mM) caused a concentration-dependent increase in 5-HT outflow, which was significantly inhibited by the removal of extracellular Ca2+. outflow induced by NaHS (0.3 mM) was also significantly inhibited by voltage-dependent L- and N-type Ca2+ channel blockers and a selective TRPA1 channel blocker. Cinnamaldehyde, a TRPA1 agonist, mimicked the secretory response to H2S. 5-HT outflow induced by Na2S3 (10 M) was also inhibited by the TRPA1 channel blocker. Furthermore, the expression of TRPA1 was localized to 5-HT-containing chemoreceptor cells in the aortic wall. These findings suggest that the activation of TRPA1 and voltage-dependent Ca2+ channels is involved in H2S-evoked 5-HT release from chemoreceptor cells in the chicken aorta. (C) 2016 Elsevier Inc. All rights reserved

Endogenous 5-HT outflow from chicken aorta by 5-HT uptake inhibitors and amphetamine derivatives

Chemoreceptor cells aggregating in clusters in the chicken thoracic aorta contain 5-hydroxytryptamine (5-HT) and have voltage dependent ion channels and nicotinic acetylcholine receptors, which are characteristics typically associated with neurons. The aim of the present study was to investigate the effects of 5-HT uptake inhibitors, fluvoxamine, fluoxetine and clomipramine (CLM), and amphetamine derivatives, p-chloroamphetamine (PCA) and methamphetamine (MET), on endogenous 5-HT outflow from the isolated chick thoracic aorta in vitro. 5-HT was measured by using a HPLC system with electrochemical detection. The amphetamine derivatives and 5-HT uptake inhibitors caused concentration-dependent increases in endogenous 5-HT outflow. PCA was about ten times more effective in eliciting 5-HT outflow than MET. The 5-HT uptake inhibitors examined had similar potency for 5-HT outflow. PCA and CLM increased 5-HT outflow in a temperature-dependent manner. The outflow of 5-HT induced by PCA or 5-HT uptake inhibitors was independent of extracellular Ca2+ concentration. The 5-HT outflow induced by CLM, but not that by PCA, was dependent on the extracellular NaCl concentration. These results suggest that the 5-HT uptake system of 5-HT-containing chemoreceptor cells in the chicken thoracic aorta has characteristics similar to those of 5-HT-containing neurons in the mammalian central nervous system (CNS)

Change and adaptation : A transnational study of takeout beverage franchise chain business of Taiwan migration to in Mongolia

[[abstract]]Over the past 20 years Mongolia has transformed itself from a socialist country to a vibrant multiparty democracy with a booming economy Since then many foreign cultures are influencing Mongolian people perceptions enormously For instance the Western and Asian food industry culture became prevalent throughout the capital city of Ulaanbaatar by ways of popular TV series shows intensive tourists and investors flows heading toward wild nature to explore nature resources making many Mongolian become more interested in a variety of foreign cuisine culture fashion style brands quality and healthy beverage options Such new chains of service contribute not only to people’s consumption but also to rapid growth of the retail chain business and take-out beverage industry In recent years the hospitality and service industry have achieved rapid growths The beverage franchise chain in the food service industry in particular has dominated the consumer market Food and beverage industry in Taiwan grows rapidly The growth rate reached 3 6% in 2012 and the market scale hit NT$385 5billion In addition the Takeout Beverage Franchise Chain plays an extremely significant role in the overall beverage market and possesses great development potentials Although this industry continues to undergo prosperous developments changes in consumption pattern consumer awareness and value force business operators to ponder upon ways to increase market share and profitability This study has combined discussions in literatures reviews and questionnaire survey results obtained from interview with consumer group brand marketing location selection Satisfaction level product packaging price experts in establishing this “Change and adaptation: A Transnational Study of Takeout beverage franchise chain business of Taiwan Migrations in The Mongolia The study conclusions shall serve as operator reference for business performance assessment in the future They shall also serve as reference for operators who intend to partake in takeout beverage franchise Hopefully it will contribute positively to the overall practical operations in the industry for globalizing Taiwanese tea business[[abstract]]Over the past 20 years Mongolia has transformed itself from a socialist country to a vibrant multiparty democracy with a booming economy Since then many foreign cultures are influencing Mongolian people perceptions enormously For instance the Western and Asian food industry culture became prevalent throughout the capital city of Ulaanbaatar by ways of popular TV series shows intensive tourists and investors flows heading toward wild nature to explore nature resources making many Mongolian become more interested in a variety of foreign cuisine culture fashion style brands quality and healthy beverage options Such new chains of service contribute not only to people’s consumption but also to rapid growth of the retail chain business and take-out beverage industry In recent years the hospitality and service industry have achieved rapid growths The beverage franchise chain in the food service industry in particular has dominated the consumer market Food and beverage industry in Taiwan grows rapidly The growth rate reached 3 6$385 5billion In addition the Takeout Beverage Franchise Chain plays an extremely significant role in the overall beverage market and possesses great development potentials Although this industry continues to undergo prosperous developments changes in consumption pattern consumer awareness and value force business operators to ponder upon ways to increase market share and profitability This study has combined discussions in literatures reviews and questionnaire survey results obtained from interview with consumer group brand marketing location selection Satisfaction level product packaging price experts in establishing this “Change and adaptation: A Transnational Study of Takeout beverage franchise chain business of Taiwan Migrations in The Mongolia The study conclusions shall serve as operator reference for business performance assessment in the future They shall also serve as reference for operators who intend to partake in takeout beverage franchise Hopefully it will contribute positively to the overall practical operations in the industry for globalizing Taiwanese tea busines

Accumulation of Metals in the Environment and Grazing Livestock near A Mongolian Mining Area

The Mongolian economy is supported by rich deposits of natural resources, such as copper, coal, and gold. However, the risk of heavy metal pollution to livestock and human have been recently discussed. This research collected various samples from soil and animal (sheep, goat, horse, cow, and camel), blood and organs (kidney and liver) in the Mongolian countryside. These samples were processed, and the concentration of metals was quantified using inductively coupled plasma-mass spectrometry (ICP/MS). As previously reported, arsenic was found at high levels of accumulation in soil. Selenium is another concern, as median concentration in one area exceeded the maximum allowable level. Cadmium and selenium were found to be highly accumulated in animal kidney. This research revealed the current pollution level in Mongolia based on evaluation of soil and animals. The concentration in animals could not indicate that animals had severe effects because of heavy metal exposure. However, kidney is eaten in Mongolia, and so there is a direct connection to human health, and this research suggested the possible risks posed by each edible animal. In particular, evaluation of metals in livestock is rare in Mongolia. This result can contribute to animal and human health in Mongolian communities