Valores antropométricos en niños con celiaquía diagnosticados precozmente

Peer Reviewe

Mating behaviour and vegetative compatibility in Spanish populations of Botryotinia fuckeliana

Mating behaviour and vegetative compatibility were studied in Spanish populations of Botryotinia fuckeliana. Fifty-seven isolates out of the 61 tested were sexually fertile with one or more of the reference strains of known mating type (MAT1-1 or MAT 1-2). Thirty-nine isolates were heterothallic, giving fertile crosses when mated with the MAT1-1 (24 isolates) or the MAT1-2 (15 isolates) reference strain. Eighteen isolates crossed successfully with both MAT1-1 and MAT1-2 reference strains, and were referred to as homothallic or MAT1-1/2. Both mating types were widespread, being represented in isolates from two regions, from the same and different greenhouses, from different hosts, and from different years of isolation. Isolates were paired on Malt Extract Agar + NaCl to evaluate vegetative compatibility. Most of the paired isolates were unable to fuse and showed a different reaction of incompatibility. Nitrate-non-utilising (nit) mutants were selected by growth on a medium amended with 30-50 gl-1 potassium chlorate. Over 600 chlorate-resistant sectors were recovered from 40 isolates at a mean frequency of 0.15-2.39 sectors per colony, but only 11% were identified as nit mutants by their thin growth with no aerial mycelium on minimal medium. However, most of these nit mutants reverted to wild type during six months of storage on chlorate-amended medium. Genetic complementation between nit mutants occurred only in two cases between mutants from the same isolate

Storage of Botrytis cinerea using different methods

Different methods were used to store a collection of field isolates of B. cinerea. Five isolates each of the fungus were stored (i) in silica gel at 4°C, (ii) in sand at 4°C, (iii) in potato dextrose agar slants at 4°C, (iv) as dry spores in tubes at -20°C, or (v) as spores in glycerol (20%) at -20°C. Different characteristics were tested after 1, 2 and 4 years of storage. Viability was poor when spores were stored dried, and contamination was frequent when stored in potato dextrose agar. Linear growth rates of cultures stored for 4 years were similar to initial values, except for one isolate in sand (9% reduction) and for another in glycerol (14% reduction). Sclerotia size and sporulation were reduced after 4 years of storage, independently of the method used. Fungicide resistance to procymidone changed to sensitive in all the isolates, except for one isolate, after four years of storage, also regardless of the storage method used. Storage in sand at 4°C or in glycerol at -20°C were the best methods for preserving B. cinerea isolates