Situating organizational action: the relational sociology of organizations

This paper advances a relational sociology of organization that seeks to address concerns over how organizational action is understood and situated. The approach outlined here is one which takes ontology seriously and requires transparency and consistency of position. It aims at causal explanation over description and/or prediction and seeks to avoid pure voluntarism or structural determinism in such explanation. We advocate relational analysis that recognizes and engages with connections within and across organization and with wider contexts. We develop this argument by briefly reviewing three promising approaches: relational pragmatism, the social theorizing of Bourdieu and critical realism, highlighting their ontological foundations, some similarities and differences and surfacing some methodological issues. Our purpose is to encourage analysis that explores the connections within and between perspectives and theoretical positions. We conclude that the development of the field of organization theory will benefit from self conscious and reflexive engagement and debate both within and across our various research positions and traditions only if such debates are conducted on the basis of holistic evaluations and interpretations that recognize (and value) difference

Power, mixing and flow dynamics of the novel Allegro™ stirred tank reactor

The development of new biopharmaceuticals relies on robust scale-up from small-scale screening studies to industrial bioreactors. Novel SUB designs can prove highly beneficial, although they lack the extensive characterisation of traditional STRs. Pall Corporation's Allegro™ STR 50–2000 L bioreactor range has a unique design of square cross-section, with three wedge-shaped baffles. A scale-down 1 L prototype (abbreviated A-STR) was developed for characterisation studies and compared to a standard cylindrical STR (abbreviated S-STR). Agreement of power and mixing number data with that of the Allegro STR 200 L indicated successful scale-down. In down-pumping mode, mixing times in the A-STR were approximately 53 % lower than in the S-STR. However, in up-pumping mode both configurations exhibited similar mixing times. This study utilises a scale-down prototype to provide quantitative data on the commercial Allegro STR bioreactor range, to define operating parameters for enhanced scalability, and for comparison with a standard bioreactor geometry

Suspension and flow dynamics of the Allegro™ stirred tank reactor

Microcarrier suspension performance and ensemble-averaged flow fields at the just-suspended condition are studied in a scale-down mimic of the Allegro STR single-use bioreactor range (abbreviated A-STR) and compared to an equivalent standard cylindrical configuration (abbreviated S-STR). Four commercial microcarriers were assessed to determine the required impeller agitation rate and corresponding power input per unit volume to achieve suspension – Cytodex™ 1, Cytodex™ 3, Cytopore™ 1, and Hillex® II microcarriers. The A-STR operating in down-pumping mode (DP) achieved homogenous microcarrier suspension at considerably lower power input when compared to the other baffled configurations. For the A-STR (DP), the power input per unit volume demand was 67.8 % lower than the A-STR in up-pumping mode (UP), 77% lower than the S-STR (DP) and 66.8 % lower than the S-STR (UP). Flow structures investigated at the just-suspended condition, in the transitional regime, were in line with those previously presented in the turbulent regime at considerably higher Reynolds number. This indicates that the normalised flow structures presented in this work are representative of those in the commercial Allegro STR range, despite operating at significantly reduced scale and Reynolds number. Removal of baffles resulted in substantially reduced power input required to achieve homogenous suspension in all configurations – other than the A-STR (DP), which exhibited relatively similar performance to the corresponding baffled configuration. Considering adherent cell sensitivity and mixing implications, and based on the results found in this work, the baffled A-STR (DP) is recommended for achieving microcarrier suspension at the lowest specific power input

Realising Value from Big Data Technology Adoption: Understanding the Role of Organisational Capabilities in the Affordance Actualization Process

The adoption of big data technologies presents organisations with many value creation opportunities that can transform and improve their business. Much of the research today focuses on big data value creation (what value big data technologies offer), whereas limited research focuses on big data value realisation (how big data value is realized). We aim to fill this research gap by addressing the following research question: how do organisations effectively realize value from the adoption of a new big data technology? We do so by adopting an affordance theory lens and empirically examine the adoption of smart meters (a big data technology) in the UK energy sector. We introduce the concept of actualization enablers, and our findings provide empirically grounded insights into the role of organisational capabilities and actualization enablers in the big data value realization process (affordance actualization). Furthermore, our findings provide important and relevant theoretical and managerial implications

CaMKII-dependent responses to ischemia and reperfusion challenges in the heart

Ischemic heart disease is a leading cause of death, and there is considerable imperative to identify effective therapeutic interventions. Cardiomyocyte Ca2+ overload is a major cause of ischemia and reperfusion injury, initiating a cascade of events culminating in cardiomyocyte death, myocardial dysfunction, and occurrence of lethal arrhythmias. Responsive to fluctuations in intracellular Ca2+, Ca2+/calmodulin-dependent protein kinase II (CaMKII) has emerged as an enticing therapeutic target in the management of ischemic heart injury. CaMKII is activated early in ischemia and to a greater extent in the first few minutes of reperfusion, at a time when reperfusion arrhythmias are particularly prominent. CaMKII phosphorylates and upregulates many of the key proteins involved in intracellular Na+ and Ca2+ loading in ischemia and reperfusion. Experimentally, selective inhibition of CaMKII activity reduces cardiomyocyte death and arrhythmic incidence post-ischemia. New evidence is emerging that CaMKII actions in ischemia and reperfusion involve specific splice variant targeted actions, selective and localized post-translational modifications, and organelle-directed substrate interactions. A more complete mechanistic understanding of CaMKII mode of action in ischemia and reperfusion is required to optimize intervention opportunities. This review summarizes the current experimentally derived understanding of CaMKII participation in mediating the pathophysiology of the heart in ischemia and in reperfusion, and highlights priority future research directions.Centro de Investigaciones Cardiovasculare

Construction of a highly enriched marsupial Y chromosome-specific BAC sub-library using isolated Y chromosomes

The Y chromosome is perhaps the most interesting element of the mammalian genome but comparative analysis of the Y chromosome has been impeded by the difficulty of assembling a shotgun sequence of the Y. B AC-based sequencing has been successful for the human and chimpanzee Y but is difficult to do efficiently for an atypical mammalian model species (Skaletsky et al. 2003, Kuroki et al. 2006). We show how Y-specific sub-libraries can be efficiently constructed using DNA amplified from microdissected or flow-sorted Y chromosomes. A Bacterial Artificial Chromosome (BAC) library was constructed from the model marsupial, the tammar wallaby (Macropus eugenii). We screened this library for Y chromosome-derived BAC clones using DNA from both a microdissected Y chromosome and a flow-sorted Y chromosome in order to create a Y chromosome-specific sub-library. We expected that the tammar wallaby Y chromosome should detect ∼100 clones from the 2.2 times redundant library. The microdissected Y DNA detected 85 clones, 82% of which mapped to the Y chromosome and the flow-sorted Y DNA detected 71 clones, 48% of which mapped to the Y chromosome. Overall, this represented a ∼330-fold enrichment for Y chromosome clones. This presents an ideal method for the creation of highly enriched chromosome-specific sub-libraries suitable for BAC-based sequencing of the Y chromosome of any mammalian species

Foundation and empire : a critique of Hardt and Negri

In this article, Thompson complements recent critiques of Hardt and Negri's Empire (see Finn Bowring in Capital and Class, no. 83) using the tools of labour process theory to critique the political economy of Empire, and to note its unfortunate similarities to conventional theories of the knowledge economy