First results of Waterborne Geophysical surveys around the Malpasso site (Tuoro sul Trasimeno, Italy) for geological and archeological characterization

With the aims of both reconstructing the geological setting of the area around the Malpasso site and to eventually find some localized remains of the battle we carried out several waterborne geophysical surveys on the area. Adopted methodologies were: magnetic surveys, seismic reflection Chirp Sonar surveys and Continuous Vertical Electric Soundings (CVES) profiles

Effect of diindolylmethane supplementation on low-grade cervical cytological abnormalities: double-blind, randomised, controlled trial

This work was supported by a Cancer Research UK project grant (C8162/A4609 project costs) and a programme grant (C8162/A10406)

Otro color en mí

Curso de Especial interés: Psicología y sexualidadEl presente trabajo es una investigación descriptiva mediante la aplicación de instrumentos como lo son encuestas. Tiene como objetivo promover por medio de un espacio virtual informar a niños, niñas, jóvenes y adolescentes acerca de la menarquia y la torarquia, que les permita comprender los procesos y cambios físicos y psicológicos que se presentan en el desarrollo del ser humano durante la etapa de la pubertad. El producto consistió en el diseño y validación de una página web en la cual los niños, niñas y adolescentes encontrarán información sobre varios procesos, principalmente sobre la menarquia y la torarquia, allí también podrán enviar sus dudas y estas serán resueltas por profesionales en el tema. La muestra se encuentra conformada por jóvenes estudiantes entre los 18 a 29 años de la ciudad de Bogotá. Uno de los hallazgos más relevantes es que las participantes tuvieron su primera menstruación (menarquia) en un rango de edad entre los 8 a 16 años, siendo esto una variable muy importante para la investigación, mientras que en el caso de los hombres se evidenció que tuvieron su primera eyaculación entre los 9 a 16 años de edad.RESUMEN 1. JUSTIFICACIÓN 2. MARCO TEÓRICO 3. METODOLOGÍA 4. OBJETIVOS 5. DISEÑO 6. INSTRUMENTOS 7. PROCEDIMIENTO 8. ASPECTOS ÉTICOS 9. ESTUDIO DE MERCADEO 10. RESULTADOS CONCLUSIONES REFERENCIAS ANEXOSPregradoPsicólog

Five-Year Safety and Performance Results from the Argus II Retinal Prosthesis System Clinical Trial

Purpose: The Argus II Retinal Prosthesis System (Second Sight Medical Products, Inc, Sylmar, CA) was developed to restore some vision to patients blind as a result of retinitis pigmentosa (RP) or outer retinal degeneration. A clinical trial was initiated in 2006 to study the long-term safety and efficacy of the Argus II System in patients with bare or no light perception resulting from end-stage RP. / Design: Prospective, multicenter, single-arm clinical trial. Within-patient controls included the nonimplanted fellow eye and patients' native residual vision compared with their vision with the Argus II. / Participants: Thirty participants in 10 centers in the United States and Europe. / Methods: The worse-seeing eye of blind patients was implanted with the Argus II. Patients wore glasses mounted with a small camera and a video processor that converted images into stimulation patterns sent to the electrode array on the retina. / Main Outcome Measures: The primary outcome measures were safety (the number, seriousness, and relatedness of adverse events) and visual function, as measured by 3 computer-based, objective tests. Secondary measures included functional vision performance on objectively scored real-world tasks. / Results: Twenty-four of 30 patients remained implanted with functioning Argus II Systems at 5 years after implantation. Only 1 additional serious adverse event was experienced after the 3-year time point. Patients performed significantly better with the Argus II on than off on all visual function tests and functional vision tasks. / Conclusions: The 5-year results of the Argus II trial support the long-term safety profile and benefit of the Argus II System for patients blind as a result of RP. The Argus II is the first and only retinal implant to have market approval in the European Economic Area, the United States, and Canada

A New Strategy to Identify and Annotate Human RPE-Specific Gene Expression

Background: To identify and functionally annotate cell type-specific gene expression in the human retinal pigment epithelium (RPE), a key tissue involved in age-related macular degeneration and retinitis pigmentosa. Methodology: RPE, photoreceptor and choroidal cells were isolated from selected freshly frozen healthy human donor eyes using laser microdissection. RNA isolation, amplification and hybridization to 44 k microarrays was carried out according to Agilent specifications. Bioinformatics was carried out using Rosetta Resolver, David and Ingenuity software. Principal Findings: Our previous 22 k analysis of the RPE transcriptome showed that the RPE has high levels of protein synthesis, strong energy demands, is exposed to high levels of oxidative stress and a variable degree of inflammation. We currently use a complementary new strategy aimed at the identification and functional annotation of RPE-specific expressed transcripts. This strategy takes advantage of the multilayered cellular structure of the retina and overcomes a number of limitations of previous studies. In triplicate, we compared the transcriptomes of RPE, photoreceptor and choroidal cells and we deduced RPE specific expression. We identified at least 114 entries with RPE-specific gene expression. Thirty-nine of these 114 genes also show high expression in the RPE, comparison with the literature showed that 85% of these 39 were previously identified to be expressed in the RPE. In the group of 114 RPE specific genes there was an overrepresentation of genes involved in (membrane) transport, vision and ophthalmic disease. More fundamentally, we found RPE-specific involvement in the RAR-activation, retinol metabolism and GABA receptor signaling pathways. Conclusions: In this study we provide a further specification and understanding of the RPE transcriptome by identifying and analyzing genes that are specifically expressed in the RPE