The management of water resources between traditions and sustainability: the qanats of Shahrood Province (North-eastern Iran)

Qanats, Iran, multidisciplinary study, water supply

Groundwater flow and geochemical modeling of the Acque Albule thermal basin (Central Italy): a conceptual model for evaluating influences of human exploitation on flowpath and thermal resource availability

Although the Acque Albule Basin has been studied since the middle of the 19th century, a comprehensive geologic conceptual model of the area has not yet been developed. The natural setting has been heavily modified by anthropic activities. Rapid evolution during the last 25 years has caused many interferences, which have led to a drastic increase of the hazards and linked risks, mainly related to water resource overexploitation and subsidence. The implementation of an exhaustive framework has become mandatory for environmental and management purposes. Starting from a critical review of previous studies, hydrogeologic and hydrogeochemical surveys and related numerical modeling have been carried out in order to achieve a quantitative understanding of the active phenomena and processes. Several hydrogeologic issues have been addressed concerning aquifer recharge areas and the different flowpaths of groundwater in respect to their division into a shallow and a deep circuit. Account has been taken of the groundwater chemistry as a function of water—rock interactions and mixing processes with uprising fluids. Different scenarios of groundwater flow in the Acque Albule aquifer have been built, using previously available piezometric measurements and the hydrodynamic parameters determined by in situ tests. These results led to the formulation of an updated hydrogeologic conceptual model to be further implemented, in which past, present and future anthropic instances and the potential of natural resources of the area have been included and taken into account. A sound conceptual model must rely on the design and development of a logical geo-database in which information is stored, updated and processed. This operational framework can result in a useful tool for land management, surveys planning and design, hazard and risk evaluation, identification of best practices and economic development of the area

The KINDRA project. Sharing and evaluating groundwater research and knowledge in Europe

Groundwater knowledge and research in the European Union is often scattered and non-standardised, because of different subjects involved and different approaches from Member States. The Horizon2020 project KINDRA has conducted an EU-wide assessment of existing groundwater-related practical and scientific knowledge based on a new Hydrogeological Research Classification System, identifying more than 280 keywords related to three main categories (namely Operational Actions, Research topics and Societal Challenges) to be intersected in a 3D-diagram approach. The classification is supported by a web-service, the European Inventory of Groundwater Research, which acts not only as knowledge repository but also as a tool to help identify relevant researchm topics, existing research trends and critical research challenges. The records have been uploaded during the project by 20 national experts from National Associations of Geologists, under the umbrella of the European Federation of Geologists. The total number of metadata included in the inventory at the end of the project are about 2300, and the analysis of the results is considered useful for producing synergies, implementing policies and optimising water management in Europe. By the use of additional indicators, the database content has been analysed by occurrence of keywords, type of document, level of innovation. Using the three-axes classification, more easily understandable by 2D diagrams as bubble plots, occurrence and relationship of different topics (main categories) in groundwater research have been highlighted. This article summarizes the activities realized in relation to the common classification system and to the metadata included in the EIGR, showing the distribution of thecollected information in different categories and attributes identified by the classification

Relationship between rainfall and water table in a coastal aquifer: the case study of Castelporziano presidential estate

This study is focused on the analysis of seasonal and annual variability in groundwater levels of the coastal aquifer of Castelporziano Presidential Estate, a protected area of 59 Km2 located in the periphery of Rome. A comparison with the local trends of rainfall at “Castello” gauging station at different time scales (monthly, seasonal and annual) has been carried out. The results highlight differences between the coastal area and eastern and northern sector of the Estate. Indeed, the seasonal effect due to local meteoric recharge is direct and regular during the year in the coastal area in respect to the eastern and northern sectors of the Estate. Moreover, annual steady regime and multi-year trend of groundwater levels suggest the contribution from the adjacent volcanic aquifer of Albani Hills. In the latter case, the regional circulation of groundwater is affected by the effects of intense withdrawals. The maintenance of the monitoring network will allow to define the flow paths of the groundwater that characterize the coastal aquifer of Castelporziano

TLR3 essentially promotes protective class I–restricted memory CD8+ T-cell responses to Aspergillus fumigatus in hematopoietic transplanted patients

Aspergillus fumigatus is a model fungal pathogen and a common cause of severe infections and diseases. CD8+ T cells are present in the human and murine T-cell repertoire to the fungus. However, CD8+ T-cell function in infection and the molecular mechanisms that control their priming and differentiation into effector and memory cells in vivo remain elusive. In the present study, we report that both CD4+ and CD8+ T cells mediate protective memory responses to the fungus contingent on the nature of the fungal vaccine. Mechanistically, class I MHC-restricted, CD8+ memory T cells were activated through TLR3 sensing of fungal RNA by cross-presenting dendritic cells. Genetic deficiency of TLR3 was associated with susceptibility to aspergillosis and concomitant failure to activate memory-protective CD8+ T cells both in mice and in patients receiving stem-cell transplantations. Therefore, TLR3 essentially promotes antifungal memory CD8+ T-cell responses and its deficiency is a novel susceptibility factor for aspergillosis in high-risk patients.These studies were supported by the Specific Targeted Research Project ALLFUN (FP7-HEALTH-2009 contract number 260338 to L.R.), by SYBARIS (FP7-HEALTH-2009 contract number 242220 to L.R.), and by the Italian Project AIDS 2010 by the Istituto Superiore di Sanita (contract number 40H40 to L.R.). A.C. and C.C. were supported by fellowships from Fundacao para a Ciencia e Tecnologia, Portugal (contracts SFRH/BPD/46292/2008 and SFRH/BD/65962/2009, respectively)

AI is a viable alternative to high throughput screening: a 318-target study

: High throughput screening (HTS) is routinely used to identify bioactive small molecules. This requires physical compounds, which limits coverage of accessible chemical space. Computational approaches combined with vast on-demand chemical libraries can access far greater chemical space, provided that the predictive accuracy is sufficient to identify useful molecules. Through the largest and most diverse virtual HTS campaign reported to date, comprising 318 individual projects, we demonstrate that our AtomNet® convolutional neural network successfully finds novel hits across every major therapeutic area and protein class. We address historical limitations of computational screening by demonstrating success for target proteins without known binders, high-quality X-ray crystal structures, or manual cherry-picking of compounds. We show that the molecules selected by the AtomNet® model are novel drug-like scaffolds rather than minor modifications to known bioactive compounds. Our empirical results suggest that computational methods can substantially replace HTS as the first step of small-molecule drug discovery

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Hydrogeological events in Fall 2016

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Hydrogeological events in Fall 2016

Not available