Engineering Microbial Consortia for High-Performance Cellulosic Hydrolyzates-Fed Microbial Fuel Cells

Microbial fuel cells (MFCs) are eco-friendly bio-electrochemical reactors that use exoelectrogens as biocatalyst for electricity harvest from organic biomass, which could also be used as biosensors for long-term environmental monitoring. Glucose and xylose, as the primary ingredients from cellulose hydrolyzates, is an appealing substrate for MFC. Nevertheless, neither xylose nor glucose can be utilized as carbon source by well-studied exoelectrogens such as Shewanella oneidensis. In this study, to harvest the electricity by rapidly harnessing xylose and glucose from corn stalk hydrolysate, we herein firstly designed glucose and xylose co-fed engineered Klebsiella pneumoniae-S. oneidensis microbial consortium, in which K. pneumoniae as the fermenter converted glucose and xylose into lactate to feed the exoelectrogens (S. oneidensis). To produce more lactate in K. pneumoniae, we eliminated the ethanol and acetate pathway via deleting pta (phosphotransacetylase gene) and adhE (alcohol dehydrogenase gene) and further constructed a synthesis and delivery system through expressing ldhD (lactate dehydrogenase gene) and lldP (lactate transporter gene). To facilitate extracellular electron transfer (EET) of S. oneidensis, a biosynthetic flavins pathway from Bacillus subtilis was expressed in a highly hydrophobic S. oneidensis CP-S1, which not only improved direct-contacted EET via enhancing S. oneidensis adhesion to the carbon electrode but also accelerated the flavins-mediated EET via increasing flavins synthesis. Furthermore, we optimized the ratio of glucose and xylose concentration to provide a stable carbon source supply in MFCs for higher power density. The glucose and xylose co-fed MFC inoculated with the recombinant consortium generated a maximum power density of 104.7 ± 10.0 mW/m2, which was 7.2-folds higher than that of the wild-type consortium (12.7 ± 8.0 mW/m2). Lastly, we used this synthetic microbial consortium in the corn straw hydrolyzates-fed MFC, obtaining a power density 23.5 ± 6.0 mW/m2

Perturbation Of Frames And Riesz Bases In Hilbert C\u3csup\u3e*\u3c/sup\u3e-Modules

We extend the Casazza-Christensen general perturbation theorem for Hilbert space frames to modular frames in Hilbert C*-modules. In the Hilbert space setting, under the same perturbation condition, the perturbation of any Riesz basis remains to be a Riesz basis. However, this result is no longer true for Riesz bases in Hilbert C*-modules. We obtain a necessary and sufficient condition under which the perturbation (under Casazza-Christensen\u27s perturbation condition) of Riesz bases of Hilbert C*-modules remains to be Riesz bases

Perturbation Of Frames And Riesz Bases In Hilbert C\u3csup\u3e*\u3c/sup\u3e-Modules

We extend the Casazza-Christensen general perturbation theorem for Hilbert space frames to modular frames in Hilbert C*-modules. In the Hilbert space setting, under the same perturbation condition, the perturbation of any Riesz basis remains to be a Riesz basis. However, this result is no longer true for Riesz bases in Hilbert C*-modules. We obtain a necessary and sufficient condition under which the perturbation (under Casazza-Christensen\u27s perturbation condition) of Riesz bases of Hilbert C*-modules remains to be Riesz bases

Riesz Bases And Their Dual Modular Frames In Hilbert C\u3csup\u3e*\u3c/sup\u3e-Modules

We investigate dual frames of modular frames and Riesz bases in Hilbert C*-modules. In Hilbert C*-modules, a Riesz basis may have many dual modular frames, and it may even admit two different dual modular frames both of which are Riesz bases. We characterize those Riesz bases that have unique duals. In addition, we obtain a necessary and sufficient condition for a dual of a Riesz basis to be again a Riesz basis, and prove some new related results. © 2008

Riesz Bases And Their Dual Modular Frames In Hilbert C\u3csup\u3e*\u3c/sup\u3e-Modules

We investigate dual frames of modular frames and Riesz bases in Hilbert C*-modules. In Hilbert C*-modules, a Riesz basis may have many dual modular frames, and it may even admit two different dual modular frames both of which are Riesz bases. We characterize those Riesz bases that have unique duals. In addition, we obtain a necessary and sufficient condition for a dual of a Riesz basis to be again a Riesz basis, and prove some new related results. © 2008

A Wireless Multisensor Node for Long-Term Environmental Parameters Monitoring

Environmental quality is a great concern to everyone, in order to realize the collection, upload, management, and visualization of parameters of atmospheric environment in real time. We propose a cheap, low-power, and fast deployment wireless sensor node for environmental monitoring, consisting of STM32 MCU, ESP8266, light sensor, rain sensor, UV sensor, seven-in-one sensor (including temperature, humidity, PM2.5, PM10, CO2, formaldehyde, and TVOC), and solar automatic tracking module. A customized μC/OS-III runs on the node, which controls the transmission of environment parameters collected by each sensor to the cloud server through the wireless network, and then the server receives, stores, and visualizes the data. In actual test, the node collects data once an hour, and the running power of the node is low and stable. Experimental results show that the node could achieve accurate collection and transmission and display the environmental data, and solar automatic tracking module could meet long-term running of the node in the night and continuous rainy days

OK-432 (Sapylin) Reduces Seroma Formation After Axillary Lymphadenectomy in Breast Cancer

Purpose/aim: Modified radical mastectomy is the standard surgery for breast cancer in developing countries. However, seroma formation regarded as the most frequent postoperative complication limits the therapeutic benefit of mastectomy and axillary surgery. The purpose of this study was to evaluate the efficacy of OK-432 in reducing seroma formation after axillary dissection. Methods: This prospective cohort study included 80 patients with advanced breast cancer who underwent modified radical mastectomy. Patients were randomized into two groups, which differed with the OK-432 administration. N = 40 patients per group were treated with either OK-432 plus closed suction drainage or drainage-only. Result: In comparison with the drainage-only group, we found that patients in the OK-432 group had a lower drainage volume (p = .030) and a shorter duration of axillary drainage (p < .01). Besides, the use of OK-432 could reduce the incidence of seroma formation (p < .01) and the volume of seroma (p = .040). There were also significant differences in reducing the chance of evacuative punctures (p = .036) and the healing time (p < .01) between control and OK-432 group. Conclusion: OK-432 not only shortened the suction drainage duration, but also significantly reduced seroma formation as well as the needs for aspiration punctures after modified radical mastectomy