Effets de l’amprolin-300 Ws sur quelques paramètres de croissance et de reproduction chez le lapin mâle (Oryctolagus cuniculus)

Objectif : L’objectif de la présente étude a été de rechercher les effets de l’Amprolin-300, un anticoccidien largement utilisé en médecine vétérinaire, sur quelques paramètres de croissance et de reproduction chez le lapin mâle de race locale. Méthodologie et résultats : Vingt-quatre (24) lapins âgés de 5 mois environ et pesant entre 2,01 et 2,78 kg ont été répartis en 4 lots comparables de 6 lapins chacun. Ils ont été traités pendant 60 jours avec différentes doses d’Amprolin-300 ws via l’eau de boisson : 0 ; 30 ; 60 et 110 mg/kg de poids corporel. Les résultats obtenus ont révélé que la consommation d’eau a significativement (p<0,05) diminué chez les lapins traités aux doses 60 et 110 mg/kg de poids corporel par rapport aux témoins. Le gain de poids et le gain moyen quotidien ont diminué de manière significative (p<0,05) chez les animaux traités aux doses 30 et 60 mg/kg de poids corporel par rapport aux animaux du lot témoin. Par contre, l’indice de consommation a diminué de façon significative (p<0,05) chez les animaux témoins et chez ceux qui ont ingéré la plus forte dose d’Amprolin-300 ws (110 mg/kg de poids corporel) comparé aux lapins traités avec les doses 30 et 60 mg/kg respectivement. Le pH de la semence a significativement (p<0,05) augmenté chez les mâles traités aux doses 30 et 60 mg/kg de poids corporel par rapport Aux mâles du lot témoin. La motilité individuelle et la concentration des spermatozoïdes ont diminué de manière significative (p<0,05) chez les lapins qui ont ingéré la plus forte dose d’anticoccidien par rapport aux lapins témoins. Conclusion et application des résultats : Ainsi, à la dose de 110 mg/kg de poids corporel, l’Amprolin-300 ws a réduit la consommation alimentaire, le gain de poids, le gain moyen quotidien, le poids spécifique et le pH de la semence. Cette dose a également inhibé la spermatogenèse et réduit le pourcentage de spermatozoïdes mobiles. Son usage devrait donc être règlementé.Mots clés : Amprolin-300 ws, lapin mâle, production et fertilité.Objective: In this study, the effects of Amprolin-300 ws, an anticoccidiostat abundantly used in veterinary medicine, on some growth and reproductive parameters in male rabbits were investigated. Methodology and results: Twenty four (24) rabbits aged 5 months and weighing between 2.01 and 2.78 kg were divided into 4 comparable groups of six rabbits each and treated for 60 days with different doses of Amprolin-300 ws from drinking water: 0, 30, 60 and 110 g/kg of body weight. The results showed that water consumption was significantly (p<0.05) decreased in rabbits treated with 60 and 110 mg/kg of body weight of Amprolin-300 compared to control rabbits. Body weight gain and average daily gain decreased significantly (p<0.05) in animals treated with 30 and 60 mg/kg of body weight compared to control rabbits. On the other hand, feed efficiency decreased significantly (p<0.05) in control animals and those who ingested the highest dose (110 mg/kg of body weight) compared to rabbits treated with 30 and 60 mg/kg of body weight respectively. The pH of semen increased significantly (p<0.05) in animals treated with 30 and 60 mg/kg of body weight compared to control animals. Individual motility and sperm concentration decreased significantly (p<0.05) in rabbits who received the highest dose of Amprolin-300 compared to control rabbits. Conclusion and application of results: Thus, at the dose of 110 mg/kg body weight, Amprolinws-300 reduced in male rabbit, food consumption, body and average daily weight gain, specific weight and pH of semen. It inhibited spermatogenesis and reduced sperm mobility. Therefore its use should be regulated

Vaccine Potential of Nipah Virus-Like Particles

Nipah virus (NiV) was first recognized in 1998 in a zoonotic disease outbreak associated with highly lethal febrile encephalitis in humans and a predominantly respiratory disease in pigs. Periodic deadly outbreaks, documentation of person-to-person transmission, and the potential of this virus as an agent of agroterror reinforce the need for effective means of therapy and prevention. In this report, we describe the vaccine potential of NiV virus-like particles (NiV VLPs) composed of three NiV proteins G, F and M. Co-expression of these proteins under optimized conditions resulted in quantifiable amounts of VLPs with many virus-like/vaccine desirable properties including some not previously described for VLPs of any paramyxovirus: The particles were fusogenic, inducing syncytia formation; PCR array analysis showed NiV VLP-induced activation of innate immune defense pathways; the surface structure of NiV VLPs imaged by cryoelectron microscopy was dense, ordered, and repetitive, and consistent with similarly derived structure of paramyxovirus measles virus. The VLPs were composed of all the three viral proteins as designed, and their intracellular processing also appeared similar to NiV virions. The size, morphology and surface composition of the VLPs were consistent with the parental virus, and importantly, they retained their antigenic potential. Finally, these particles, formulated without adjuvant, were able to induce neutralizing antibody response in Balb/c mice. These findings indicate vaccine potential of these particles and will be the basis for undertaking future protective efficacy studies in animal models of NiV disease

Systematic Analysis of Sequences and Expression Patterns of Drought-Responsive Members of the HD-Zip Gene Family in Maize

Background: Members of the homeodomain-leucine zipper (HD-Zip) gene family encode transcription factors that are unique to plants and have diverse functions in plant growth and development such as various stress responses, organ formation and vascular development. Although systematic characterization of this family has been carried out in Arabidopsis and rice, little is known about HD-Zip genes in maize (Zea mays L.). Methods and Findings: In this study, we described the identification and structural characterization of HD-Zip genes in the maize genome. A complete set of 55 HD-Zip genes (Zmhdz1-55) were identified in the maize genome using Blast search tools and categorized into four classes (HD-Zip I-IV) based on phylogeny. Chromosomal location of these genes revealed that they are distributed unevenly across all 10 chromosomes. Segmental duplication contributed largely to the expansion of the maize HD-ZIP gene family, while tandem duplication was only responsible for the amplification of the HD-Zip II genes. Furthermore, most of the maize HD-Zip I genes were found to contain an overabundance of stress-related ciselements in their promoter sequences. The expression levels of the 17 HD-Zip I genes under drought stress were also investigated by quantitative real-time PCR (qRT-PCR). All of the 17 maize HD-ZIP I genes were found to be regulated by drought stress, and the duplicated genes within a sister pair exhibited the similar expression patterns, suggesting their conserved functions during the process of evolution