NURS 128: Evidence-based Nursing Practice Course Redesign

Poster summarizing course redesign activities for NURS 128: Evidence-based Nursing Practice.https://scholarworks.sjsu.edu/davinci_itcr2014/1004/thumbnail.jp

A cellulose-based bioassay for the colorimetric detection of pathogen DNA

Cellulose-paper-based colorimetric bioassays may be used at the point of sampling without sophisticated equipment. This study reports the development of a colorimetric bioassay based on cellulose that can detect pathogen DNA. The detection was based on covalently attached single-stranded DNA probes and visual analysis. A cellulose surface functionalized with tosyl groups was prepared by the N,N-dimethylacetamide-lithium chloride method. Tosylation of cellulose was confirmed by scanning electron microscopy, Fourier transform infrared spectroscopy and elemental analysis. Sulfhydryl-modified oligonucleotide probes complementary to a segment of the DNA sequence IS6110 of Mycobacterium tuberculosis were covalently immobilized on the tosylated cellulose. On hybridization of biotin-labelled DNA oligonucleotides with these probes, a colorimetric signal was obtained with streptavidin-conjugated horseradish peroxidase catalysing the oxidation of tetramethylbenzamidine by H2O2. The colour intensity was significantly reduced when the bioassay was subjected to DNA oligonucleotide of randomized base composition. Initial experiments have shown a sensitivity of 0.1 μM. A high probe immobilization efficiency (more than 90 %) was observed with a detection limit of 0.1 μM, corresponding to an absolute amount of 10 pmol. The detection of M. tuberculosis DNA was demonstrated using this technique coupled with PCR for biotinylation of the DNA. This work shows the potential use of tosylated cellulose as the basis for point-of-sampling bioassays.Peer reviewedFinal Accepted Versio

DIGITAL FINANCIAL INCLUSION IN INDIA

Digital financial inclusion refers to the internet access to use the formal financial services by excluded and underserved population. E-Banking activity in rural India results in increased usage of financial services and improved living conditions due to the technological involvement in financial inclusion. Financial inclusion, as a result of digital financial services, also promotes economic growth. The purpose of this research is to identify the factors that influence the adoption of digital financial services, as well as people\u27s intentions to use them. This will aid in determining how the correct technology and strategy may help India achieve financial inclusion. The study also tries to identify the role of digital financial inclusion in the country\u27s economic growth. This study is exploratory in nature, with an emphasis on utilizing secondary sources of data related to financial inclusion to better understand new banking technology and people\u27s perceptions on adoption and usage of banking services

Role of multidetector computed tomography in evaluation of suspected bronchogenic carcinoma

Background: Bronchogenic  carcinoma  is  the  leading cause  of  cancer  deaths  in  developed  countries  and  is  rising  at  alarming  rates  in developing countries. Deaths due to lung cancer are more than those due to colorectal, breast and prostate cancers put together.  CT remains the routine imaging procedure for determining resectability and assessing intra- and extra thoracic spread of lung cancer.Methods: 30 patients with strong clinical / radiological suspicious of bronchogenic carcinoma were included in this study. MDCT was carried out and its provisional diagnosis of bronchogenic carcinoma was correlated with pathological diagnosis obtained on bronchoscopic biopsy / CT guided FNAC.Results: Patient’s age ranged between 45 to 80 years with the mean age of 59 years. There was significant male preponderance (26 males) with smoking being the most common risk factor (83.3%). Cough (83.3%) and dyspnea (80%) were the commonest symptoms. The most common radiological manifestation was central hilar mass seen in 20 (66.6%) patients and peripheral mass seen in 10 (33.3%) patients. Provisional CT diagnosis was found in complete correlation with pathological diagnosis made on bronchoscopy biopsy/ CT guided FNAC in 28 (93.3%) patients and others 2 patients diagnosed as pulmonary tuberculosis, non caseating granulomatous inflammation on histopathological diagnosis. Squamous cell carcinoma was the commonest histopathological type seen in 16 (53.3%) patients followed by adenocarcinoma seen in 8 (26.6%) patients.Conclusions: Multidetector computed tomography plays an important role in evaluating and staging of bronchogenic carcinoma.CT had the high predictive value in evaluating bronchogenic carcinoma and found to be 93.3%.

Healthcare worker\u27s understanding of perinatal depression and maternal mental health service needs in rural Kenya

Background: Perinatal depression is a significant maternal mental health issue in sub-Saharan Africa. Because of the severe shortage of mental health specialists in this region, healthcare workers can be trained to deliver mental health services. Yet, little research has examined their views about maternal mental health. Aims: To help inform the development of a perinatal depression screening program, the aim of this pilot study was to explore the knowledge and understanding of perinatal depression among healthcare workers in rural Kenya. Methods: Fourteen healthcare workers participated in focus group interviews. Results: Content analysis of interview data yielded three primary themes: 1) healthcare workers’ knowledge and understanding of perinatal depression, 2) symptoms of perinatal depression, and 3) identification of perinatal mental health help-seeking resources and barriers in a rural community. Additionally, healthcare workers used a hierarchical approach to manage perinatal depressive symptoms, first by using available resources at the clinic, followed by psychiatric referral as indicated. Conclusions: Healthcare professionals may use study findings to enhance awareness of barriers and stigma associated with perinatal depression and to create a culturally sensitive mental health program for women in this rural community

Harnessing the Genetic Diversity and Metabolic Potential of Extremophilic Microorganisms through the Integration of Metagenomics and Single-Cell Genomics

Microorganisms thriving under extreme environments have proven to be an invaluable resource for metabolic products and processes. While studies carried out on microbial characterization of extremophilic environments during golden era of microbiology adapted a ‘reductionist approach’ and focused on isolation, purification and characterization of individual microbial isolates; the recent studies have implemented a holistic approach using both culture-dependent and culture-independent approaches for characterization of total microbial diversity of the extreme environments. Findings from these studies have unmistakably indicated that microbial diversity within extreme environments is much higher than anticipated. Consequently, unraveling the taxonomic and metabolic characteristics of microbial diversity in extreme environments has emerged as an imposing challenge in the field of microbiology and microbial biotechnology. To a great extent, this challenge has been addressed with inception and advancement of next-generation sequencing and computing methods for NGS data analyses. However, further it has been realized that in order to maximize the exploitation of genetic and metabolic diversity of extremophilic microbial diversity, the metagenomic approaches must be combined synergistically with single-cell genomics. A synergistic approach is expected to provide comprehensions into the biology of extremophilic microorganism, including their metabolic potential, molecular mechanisms of adaptations, unique genomic features including codon reassignments etc

Analytical Method Development and Validation for Assay of Rufinamide Drug

A simple, rapid, sensitive, cost effective, and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the stability testing of rufinamide. The proposed RP-HPLC method was developed on phenome-nex LunaR C-18 5μm,250 mm × 4.6 mm id. Column (at ambient temperature) and a mobile phase consisting of phosphate buffer: acetonitrile (60:40) was delivered at a flow rate of 1.0ml/ min. The analyte was detected by using a UV detector at the wavelength of 293 nm. The method was found to be linear over the concentration range of 50- 150 μgml-1 (r2=0.999). 30. The retention time of rufinamide was 4.717 min. Most searchable Keywords assay method development, analytical method development, method development, analytical method development and validation, analytical method development introduction, development and validation of analytical methods, method development and validation, assay method validation, analytical method development in pharmaceuticals, pharmaceutical method development,&nbsp

Scoping Review of Racial and Ethnic Representation of Participants in Mental Health Research Conducted in the Perinatal Period During the COVID-19 Pandemic

OBJECTIVE: To identify the racial and ethnic representation of participants in mental health research conducted in the perinatal period during the COVID-19 pandemic. DATA SOURCES: MEDLINE, CINAHL, Cochrane Library, PsycINFO, Scopus, Web of Science. STUDY SELECTION: We included peer-reviewed research articles in which researchers reported mental health outcomes of women during the perinatal period who were living in the United States or Canada during the COVID-19 pandemic. We included 25 articles in the final review. DATA EXTRACTION: We extracted the citation, publication date, design, aim, country of origin, participant characteristics, sampling method, method of measurement of race and ethnicity, and mental health outcome(s). DATA SYNTHESIS: The combined racial and ethnic representation of the 16,841 participants in the included studies was White (76.5%), Black (9.8%), other/multiracial (6.2%), Asian (3.9%), Hispanic/Latina (2.6%), Indigenous or Ethnic Minority Canadian (0.9%), and Native American or Alaska Native (0.1%). Most studies were conducted in the United States, used a cross-sectional design, and incorporated social media platforms to recruit participants. Depression, anxiety, and stress were the most frequently assessed mental health outcomes. CONCLUSION: Relatively few women of color who were pregnant or in the postpartum period during the pandemic participated in mental health research studies. Future studies should develop intentional recruitment strategies to increase participation of women of color. Researchers should use updated guidance on reporting race and ethnicity to accurately represent every participant, minimize misclassification of women of color, and report meaningful results