Evaluation of antagonistic activities of Bacillus spp. against certain bacteria of medical importance

In this work we focused on the antagonistic potential of Bacillus spp. isolates from cow dung. Out of fourteen bacterial strains, isolate KD104 and KD117 were probably identified as Bacillus spp. These two isolates were screened for their antagonistic activity against 14 test organisms viz., Vibrio Cholera (MTCC 3904), Salmonella typhi (MTCC 3216), Escherichia coli (SGPGI), Staphylococcus aureus (MTCC 7443), Bacillus subtilis (MTCC 441), Bacillus cereus (MTCC 6728), Proteus vulgaris (MTCC 426), Enterococcus faecalis (MTCC 439), Pseudomonas aeruginosa (MTCC 424), Escherichria coli (MTCC 118), Shigella flexneri (MTCC 1457), Salmonella typhimurium (MTCC 3231), Streptoccus pyogenes (MTCC 442) and Staphylocccus aureus (MTCC 3160) using cross-streak method. The preliminary screening revealed significant antimicrobial effect of both isolates against Vibrio Cholera (MTCC 3904), Salmonella typhi (MTCC 3216), Escherichia coli (SGPGI), and Bacillus cereus (MTCC 6728). Therefore, this study indicates that these Bacillus species may be up-hold to industrial level for production of antimicrobial agent, which should be further analyzed for its possibility to be used as therapeutic agent

Phytochemical Analysis And Evaluation Of Antioxidant Activity In Fagopyrum Esculentum

Herbal nutraceutical products have become increasingly popular in recent years, particularly in the dermatology and cosmetics fields, because of their potential to prevent skin photodamage and their photoprotective qualities against UV radiation. Standardized herbal extracts are necessary for modern phytopharmaceutics and phytocosmetics, and buckwheat herb, which is high in flavonoids, has shown promise as an antioxidant source. The objective of the research is to ascertain the existence of various nutraceutical components in buckwheat, such as proteins, carbohydrates, sterols, alkaloids, saponins, and tannins, both quantitatively and qualitatively. The study assesses possible interactions between these components, food, and prescribed medications as the body of research on their health benefits grows. Through a variety of phytochemical tests, the results show the high antioxidant and nutraceutical qualities of Fagopyrum esculentum, with methanolic extracts showing greater activity than water extracts. Notably, the study highlights the high antioxidant activity, alkaloids, flavonoids, tannins, and phenolic compounds of F. esculentum as potential major food supplement. This study offers a biochemical justification for its application in ethnopharmacology and as a nutraceutical to improve health and prevent a variety of ailment

Influence of Panchgavya on Growth, Yield and Quality parameters of Bitter Gourd (Momordica charantia L.)

The Present experiment aims to highlights the Influence of Panchagavya on Growth, Yield and Quality Parameters of Bitter Gourd (Momordica charantia L.). The Experiment was conducted at Horticulture Research Field, Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj during July-October, 2022. The Experiment was laid out in factorial randomized block design with twelve treatment combinations and replicated thrice. The experiment consisted of two factors. Factor 1: Hybrids as TMBI-1304, TMBI-1305 and Factor 2: Different concentrations of Panchagavya P₀: Control (water spray), P1: 1% spray of Panchagavya, P2: 2% spray of Panchagavya, P3: 3% spray of Panchagavya, P4: 4% spray of Panchagavya, P5: 5% spray of Panchagavya. Among different concentrations 5% spray of Panchagavya gave better results in Vine length (140.500 cm), Appearance of 1st male flower (33.110), Appearance of 1st female flower (35.110), Days to 50% flowering (36.157), Days to 1st harvest (49.778), Number of fruits per plant (41.556), Length of fruit (14.344 cm) Average fruit weight (153.667 g), Girth of fruit (17.678), Average fruit yield per plant (56.760), Average fruit yield per plot (5.66) TSS (4.177) and Ascorbic acid (2.050). The best interaction results were found in TMBI-1304 along with 5% spray of Panchagavya which gave maximum growth, yield and quality characteristics in bitter gourd