GC-MS ANALYSES OF LEAF AND ROOT EXTRACTS OF DIDYMOCARPUS TOMENTOSA

Objective: Didymocarpus tomentosa Wight., is a traditional medicinal plant used in the treatment of fever and skin allergy and the present study were conducted to identify the phytochemical constituents in leaf and root extracts using gas chromatography and mass spectrometry (GC-MS).Methods: The methanolic leaf and root extracts were analyzed using Shimadzu GCMS-QP 2010 gas chromatography-mass spectrometer. The mass spectrum of GC-MS was interpreted using the database of the National Institute of Standards and Technology (NIST).Results: The GC-MS analyses of leaf and root extracts revealed the presence of twenty six and twenty three phytochemical constituents respectively. 5-Hydrxoymethylfurfural (15.1%), cis, cis, cis-7,10,13-Hexadecatrienal (12.5%), Hexadecanoic acid (11.8%), Lupenone (29.1%), γ-Sitosterol (21.3%) and Lupeol (12.0%) were the major constituents. Conclusion: The leaf and root extracts of D. tomentosa possess various phytochemical constituents, which are of high therapeutic values.Â

CYTOTOXIC ACTIVITY OF METHANOLIC EXTRACTS OF SOLANUM ERIANTHUM D. DON

Objective: The study was aimed to evaluate cytotoxic activity of the methanolic extracts of Solanum erianthum on HeLa, HepG-2 and MCF-7 cell lines.Methods: The percentage viability of the cell lines were carried out using trypan blue dye exclusion method and the cytotoxicity of the extracts was evaluated by MTT assay. Result: Solanum erianthum leaf and fruit extract showed significant cytotoxic activity on the tested cell lines with IC50 values ranging from 111.67 to 385 µg mL-1. Fruit extract showed potent cytotoxic activity on MCF-7 (IC50 111.67 µgmL-1), followed by leaf extract (IC50 150.50 µgmL-1)on HeLa cell line using MTT assay. Cell viability assay showed that fruit extract was very active on MCF-7 and leaf extract was effective against HeLa. Conclusion: The experiment performed reveals that Solanum erianthum can be used in development of anticancer drug.Â